Huffmanela persica, Ghanei-Motlagh & Fast & Groman & Kumar & Soliman & El-Matbouli & Saleh, 2023

Ghanei-Motlagh, Reza, Fast, Mark D., Groman, David, Kumar, Gokhlesh, Soliman, Hatem, El-Matbouli, Mansour & Saleh, Mona, 2023, Description, molecular identification and pathological lesions of HUffmanela perSICa sp. nov. (Nematoda: Trichosomoididae: Huffmanelinae) from the daggertooth pike conger MUraeneSox CInereUS, Parasites & Vectors (182) 16 (1), pp. 1-20 : 4-5

Huffmanela persica sp. nov.

Taxonomic summary

Type definitive host: Daggertooth pike conger, Muraenesox cinereus (Forsskål) , Anguilliformes , Muraenesocidae , date of collection, February 2021.

Type locality: Persian Gulf off the coast of Zir Ahak, Bushehr, Iran (28°17’ N, 51°13’ E) .

Site of infection: Ovary, tunica serosa of stomach ( Fig. 2A ). Conspicuous masses of eggs of H. persica sp. nov. were observed in the ovaries of all 13 fish infected. Te egg aggregations were simultaneously found in the serosae of stomach of 3 out of 13 fish infected by the nematode. All fish examined were also infected by larval (viscera) and adult (stomach and intestine) forms of anisakids and raphidascaridids. Adult forms (male and female) of H. persica sp. nov. were not observed.

Prevalence: 65% (13 infected out of 20).

Etymology: Te specific name persica (Persian) refers to the country of its occurrence ( Iran).

Molecular characterization: Nucleotide sequences of the 18S rDNA (OQ418445), 28S rDNA (OQ428648) and ITS rDNA (OQ428649) of the new species have been deposited in GenBank.

Deposited specimens: Syntype eggs collected from the host ( M. cinereus ) were deposited in the Helminthological Collection , Institute of Parasitology , Biology Centre of the Czech Academy of Sciences, České Budějovice, Czech Republic (catalog no. N-1277) .

Figures

Fig. 2 Macroscopic and microscopic appearance of fully developed eggs of Huffmanela persica sp. nov. A Grossly visible lesions of eggs previously deposited by adult forms of H.persica sp. nov. in the form of dark spots of various size within the infected tissues (ovary and serosa of stomach) of Muraenesox cinereus. B Wet mount prepared from infected ovary illustrating variously oriented advanced eggs of H.persica sp.nov.within the egg clusters