Anteaglonium parvulum (W.R. Gerard) Mugambi & Huhndorf, Syst. Biodiv.

Jayasiri, Subashini C., Jones, E. B. Gareth, Kang, Ji-Chuan, Promputtha, Itthayakorn, Bahkali, Ali H. & Hyde, Kevin D., 2016, A new species of genus Anteaglonium (Anteagloniaceae, Pleosporales) with its asexual morph, Phytotaxa 263 (3), pp. 233-244 : 240-242

Anteaglonium parvulum (W.R. Gerard) Mugambi & Huhndorf, Syst. Biodiv. 7(4): 462 (2009)

Facesoffungi Number: FoF 01931

Reference specimen: MFLU 16-0472 Fig: 4

Saprobic on dead wood. Sexual morph: Hysterothecia 250–430 μm long, 200–250 μm high, 170–200 μm wide hysterothecial, superficial or sunken in substrate, oval to elongate, or globose to subglobose, black, carbonaceous, straight or curved, rarely branched, without subiculum, acuminate apices, occurring on a black thin crust, not tending to darken the substratum, without KOH extractable pigments. Ostiole central, slit-like. Peridium 35–41 μm thick, dark brown, comprising a single stratum of dark brown cells of textura epidermoidea. Hamathecium comprising 0.5–1.3

μm thin, cylindrical to filiform, hyaline, septate pseudoparaphyses in a gelatinous matrix, found between and above the asci. Asci 30–52 × 3.2–5.8 μm, 8-spored, bitunicate, fissitunicate, elongate cylindric-clavate, straight or slightly curved, short-pedicellate, apically rounded, with an ocular chamber. Ascospores 5.2–8 × 2–3.2 μm (x = 7 × 2.7 μm; n = 20),

uniseriate, 2-celled, very small, ellipsoidal, hyaline, 1-septate, constricted at septa, widest at the middle and tapering towards the narrow ends, straight, smooth-walled. Asexual morph: Coelomycetous. Conidiomata 110–121 μm high,

104–115 μm diam., pycnidial, globose, superficial to subperidermal, separate, unilocular, thick-walled. Conidiomata wall 19–23 μm wide, composed of 4–6 layers, brown colour cells textura angulari s. Conidiophores 8–13 μm long,

unbranched, hyaline, formed from the innermost layer of wall cells. Conidiogenous cells 3–5 × 3–2–3 μm, globose, hyaline, smooth, with a rounded tip. Conidia 3–5 × 2–3 μm (x = 4 × 2.5 μm; n = 20), hyaline, oval to globose, rounded at both ends, aseptate, smooth walled ( Fig. 5 ).

Colony characteristics:— Colonies on MEA, 13–16 mm diam within 7 days at 25° C, tightly arranged, with short, aerial mycelium. Four strains were isolated from this species and there are given different colonies when grow in same condition ( Fig. 6 ).

Material examined:— THAILAND. Chiang Rai: Doi Pui, dead branch of undetermined tree, 15 June 2014, Subashini C. Jayasiri, reference specim en designated here ( MFLU 16-0472, ( HKAS 91941) ( MFLU 16-0473, MFLU 16-0474, MFLU 16-0470) ex-type culture ( MFLUCC 14-0817) ( MFLUCC 14-0815, MFLUCC 14-0821, MFLUCC 14-0823).

Notes:— This species belongs to the genus Anteaglonium because of its morphological features, in particular the black, carbonaceous hysterothecia, cylindric-clavate, short-pedicellate asci, and uniseriate, hyaline, two-celled, small (less than 8 μm) ascospores. In the multigene phylogenetic analysis, it also clusters with other Anteaglonium species. In this study, four Anteaglonium parvulum strains ( MFLUCC 14-0817, MFLUCC 14-0815, MFLUCC 14- 0821, MFLUCC 14-0823) were isolated from Chiang Rai, Thailand. They are morphologically similar with previous records ( GKM 1218, SMH 5223, SMH 5210, GKM 219 N) of this species in having acuminate hysterothecial apices, lack of darkening of substrate, and absence of KOH-soluble pigments ( Boehm et al. 2009b). They also clustered with sequences strains of A. parvulum from the GenBank. Morphologically and phylogenetically these four strains ( MFLUCC 14-0817, MFLUCC 14-0815, MFLUCC 14-0821, MFLUCC 14-0823) are similar, but have different culture characteristics as illustrated in Fig. 6 . Biological and culture characters were used as criteria for separating species in plant pathogenic fungi ( Bernadovičová and Juhásová 2006), but this is not adopted in this paper.

Figures

FIGURE 5. Asexual morph of Anteaglonium parvulum from culture. a Germinated ascospore. b Above view of colony. c Reverse view of colony. d Asexual structures in culture. e Section through pycnidia. f Conidiomata wall. g, h Conidiogenous cells. i Conidia. Scale bars: b, c=4 cm, e=50 μm, f=20 μm, g–i=10 μm.

FIGURE 6. Different colony morphology of four Anteaglonium parvulum strains. a MFLUCC 14-0817 forward, b Reverse. c MFLUCC 14-0821 forward, d Reverse. e MFLUCC 14-0823 forward, f Reverse. g MFLUCC 14-0815 forward, h Reverse. Scale bars: a–h=4 cm.