Puccinia vaginatae Juel, Bot. Notis. 1892

Ji, Jingxin, Li, Zhuang, Li, Yu & Kakishima, Makoto, 2022, Phylogenetic approach for identification and life cycles of Puccinia (Pucciniaceae) species on Carex (Cyperaceae) from northeastern China, Phytotaxa 542 (3), pp. 221-255 : 237

C6: Puccinia vaginatae Juel, Bot. Notis. 1892 –1893: 56, 1893. ( Fig. 9 )

Description:— Spermogonia amphigenous, surrounded by greenish-yellow lesions, densely grouped, honey-yellow, subepidermal, flask-shaped, type 4 of Cummins & Hiratsuka (2003). Aecia is mostly hypophyllous, cupulate with peridia, Aecidium - type. Peridial cells firmly conjunct, cubic to polygonal, hyaline. Aeciospores catenulate, globose to subglobose, angular, 15.5–18.0 × 13.0–17.5 µm (av. 17.0 × 14.5 µm); walls hyaline, 0.4–0.8 µm (av. 0.6 µm) thick, densely verrucose with big granules.

Immature uredinia is rarely found. Telia hypophyllous, blackish brown, rounded to broadly elliptic, erumpent. Teliospores broadly clavate, ellipsoid, truncate, rounded or conical at apices, middle or weakly constricted at the septa, rounded or attenuate at the bases, 36.0–53.0 × 15.0–19.5 µm (av. 43.5 × 17.5 µm); walls pale brown, 0.6–1.2 µm (av. 0.8 µm) thick at sides, 6.6–13.5 µm (av. 10.0 µm) at apices; pedicels persistent, hyaline, 14.0–30.0 µm (av. 22.0 µm) long.

Specimens examined from northeastern CHINA: — Jilin Province, South of Changbai Mountain , spermogonia and aecia on Saussurea sp. , 7 July 2017, HMJAU 8891 , 8892 . Jilin Province, Yanbain City, Erdaobaihe , telia on Carex sp. , 29 July 2015, HMJAU 8893 .

Hosts and distribution in northeastern CHINA ( Fig. 1 ): —Spermogonia and aecia on Saussurea sp. (Asteraceae) (D). Telia on Carex sp. (D).

Note: —Three species, P. ohsawaensis Kakish. , P. rupestris Juel and P. vaginatae Juel , have been reported as heteroecious species alternating between Sausurrea and Carex ( Juel 1893, Jørstad 1954, Kakishima & Sato 1980, Hiratsuka et al. 1992). Among them, specimens of this clade are morphologically identical with descriptions of P. vaginatae by Juel (1893) and Jørstad (1954). This species is morphologically similar to P. rupestris , but different in the presence of spermogonia and the number of urediniospore germ pores. Teliospore size of P. ohsawaensis is larger than that of P. vaginatae ( Kakishima & Sato 1980) . Puccinia vaginatae is also morphologically similar and phylogenetically close to P. caricis-atractylodis . However, this species has a distinct phylogenetic lineage ( Fig. 3 ) and its spermogonial and aecial host is different from that of P. caricis-atractylodes . Although Zhuang et al. (1998) reported this species as a doubtful species in China, we confirm its occurrence in China.

Figures

FIGURE 1. Collection areas in northeastern China. A. Changchun area in Jilin Province. B. Jilin area in Jilin Province. C. Mountanious area in southern Heilongjiang Province. D. Area around Changbai Mountains in Jilin Province. E. Baicheng area in western Jilin Province. F. Yanbian area in eastern Jilin Province. G. Tonghua area in southeastern Jilin Province.

FIGURE 3. Phylogenetic tree of specimens on Cyperaceae and related host plants constructed by MP method based on ITS+28S regions of rDNA. Bootstrap values of MP and ML are followed by the Bayesian posterior probabilities (Bpp) on the nodes in the topology.Asterisk (*) represents bootstrap values or Bpp less than 50% in the topology. Sample data are shown with voucher specimen number or GenBank accession number, and host plant. Sequence data determined in this study are shown in color. Teliospore shapes are shown in each clade detected, and new species are shown by asterisk (*) on clades. 0, I: Spermogonial and aecial host genus. Asterisk (*) on host plants: Spermogonial and aecial host plants.

FIGURE 9. Puccinia vaginatae on Saussurea sp. (A–F) and Carex sp. (G–I). A. Plants producing spermogonia and aecia on the leaf surface in the field. B. Vertical section of a spermogonium. C. Vertical section of an aecium surrounded with peridia. D. Aecia on the lower leaf surface. E. Aeciospores with granules on the surface observed under SEM. F. An aecium observed under SEM. G. Telia on the lower leaf surface. H, I. Vertical section of a telium. Scale bars: B = 20 μm, C = 30 μm, E = 5 μm, F, H, I = 50 μm.