Marasmius nummularius Berk. & Broome, J. Linn. Soc., Bot.

Shay, Jackie E., Desjardin, Dennis E., Perry, Brian A., Grace, Chris L. & Newman, Danny S., 2017, Biodiversity and phylogeny of Marasmius (Agaricales, Basidiomycota) from Madagascar, Phytotaxa 292 (2), pp. 101-149 : 131-132

22. Marasmius nummularius Berk. & Broome, J. Linn. Soc., Bot. 14 (73): 33. 1873 (1875). ( Fig. 14 , Plate 5 )

Type:— SRI LANKA. Kandy District, Peradeniya, Thwaites 102 cum icone ( K!).

Description:— Pileus 2–7 mm diam, convex to plano-convex; margin striate; surface glabrous; orangish brown to ochraceous (5 B 8). Context thin. Lamellae adnexed, subdistant (14–16) with 3–4 series of lamellulae, white with brown to orangish brown edges. Stipe 20–48 × 1 mm, central, tough, non-insititious; surface dull, hispid; apex white, base light brown to orange-brown (6D6). Odor and taste not distinctive.

Basidiospores (10.4–) 11.2–14.4 × 3.2–5.6 μm [x mr = 12.4–12.7 × 4.3–4.8 μm; x mm = 12.56 ± 0.21 × 4.56 ± 0.39 μm; Q = 1.9–3.6; Q mr = 2.69–2.92; Q mm = 2.80 ± 0.16, n = 25, s = 2], ellipsoid, smooth, hyaline, inamyloid, thin-walled. Basidia 17.6–25.6 × 6.4–8 μm, clavate to broadly clavate or cylindrical, 4-spored, hyaline, inamyloid, thin-walled.

Basidioles 16.8–25.6 × 5.6–8.8 μm, clavate to broadly clavate, cylindrical or fusoid, hyaline, inamyloid, thin-walled.

Cheilocystidia of Siccus - type broom cells; main body 6–20 × 6–9.6 μm, broadly clavate, hyaline to pale yellow brown,

dextrinoid, thick-walled; apical setulae 0.8–10.4 × 0.8–2.4 μm, numerous, cylindrical to conical, subacute, hyaline,

inamyloid, thick-walled. Pleurocystidia scattered, not conspicuous, 22–28 × 6.5–7.5 μm, fusoid, hyaline, refractive,

inamyloid, thin-walled. Pileipellis mottled, a hymeniform layer of Siccus - type broom cells; main body 9.6–20 × 6.4–

8.8 μm, cylindrical to clavate or subglobose, hyaline to light brown, dextrinoid, thick-walled; apical setulae 1.6–12.8

× 0.5–2.4 μm, cylindrical to conical, hyaline, inamyloid, thick-walled. Pileus trama interwoven; hyphae 3.2–7.2

μm diam, cylindrical, smooth, hyaline, dextrinoid, thin-walled. Lamellar trama regular; hyphae 3.2–5.6 μm diam,

cylindrical, smooth, hyaline, weakly dextrinoid, thin-walled. Stipe tissue monomitic; cortical hyphae 2.4–4 μm diam, parallel, cylindrical, hyaline to pale yellow, dextrinoid, thick-walled; medullary hyphae 3.2–9.6 μm diam, parallel, cylindrical to inflated, hyaline, inamyloid, thick-walled. Caulosetae 28–109 × 7.2–36 μm, conical to cylindrical, fusoid or lageniform, hyaline, inamyloid, thick-walled. Clamp connections present.

Habit, habitat and known distribution:—Solitary to gregarious on leaf litter of Dalberjia ( Fabaceae ) and bark of unknown trees. Indonesia (Java), Madagascar, Sri Lanka, Thailand.

Material examined:— MADAGASCAR. Commune Ranomafana , District Ifanadiana , Region Vatovavy-Fitovinany , Ranomafana National Park, Talatakely Trail, elev. 937–973 m, GPS: 21˚ 15.237’ S, 47˚ 25.183’ E, 20 January 2014, J. E. Shay 121 ( SFSU) ; same location and date, J. E. Shay 124 ( SFSU) .

Notes:—The Madagascar material is characterized by small (2–7 mm diam) orangish brown to ochraceous pileus, subdistant orangish brown-marginate lamellae, a hispid orangish brown to brown non-insititious stipe, basidiospores with mean 12.4–12.7 × 4.3–4.8 μm, Siccus - type cheilocystidia and pileipellis cells with setulae up to 12 mm long, small fusoid pleurocystidia, rare pileosetae, conspicuous caulosetae, and growth on woody debris. Populations of M. nummularius in Thailand ( Wannathes et al. 2009a) and Indonesia ( Desjardin et al. 2000) differ in lacking the small pleurocystidia, and often have more reddish brown tones to the pileus. An ITS sequence of Madagascar material ( KX 148979) shows 98% similarity to two Thai sequences ( EU 935492, EU 935493) forming a well-supported clade (90% BS, 1.0 PP) ( Fig. 1b ). The African species M. castaneovelutinus Henn. and M. fulvovelutinus Beeli differ in forming larger (4–35 mm diam), chestnut brown to reddish brown pilei, non-marginate lamellae, larger basidiospores (14–18 × 4–6 μm), and more conspicuous pleurocystidia ( Antonín 2007).

Figures

FIGURE 1a. Maximum likelihood phylogeny based on ITS sequence data. Marasmius from Madagascar are indicated in bold type.Values separated by / refer to nonparametric ML bootstrap proportions and Bayesian posterior probabilities, respectively. Values greater than 70/0.70 are shown (- designates a value below 70% or below 0.70). Nodes receiving support values greater than 90/0.95 are represented by bold branches. G—sect. Globulares; N—sect. Neosessiles; SA—sect. Sicci ser. Atrorubentes; SL—sect. Sicci ser. Leonini; SH—sect. Sicci ser. Haematocephali.

PLATE 5. Basidiocarps representing sect. Sicci ser. Spinulosi a) Marasmius dendrosetosus (JES 205) b) Marasmius nummularius (JES 124). Scale bar = 10 mm. Photography by D.S. Newman.

FIGURE 14. Marasmius nummularius (JES 121, JES 124) a) basidiospores; b) basidia; c) basidioles; d) cheilocystidia; e) pleurocystidia; f) pileipellis cells; g) caulosetae. Scale bar = 10 μm. Illustrated by J.E. Shay.