Marasmius hinnuleus Berk. & M.A. Curtis, J. Linn. Soc., Bot.

Shay, Jackie E., Desjardin, Dennis E., Perry, Brian A., Grace, Chris L. & Newman, Danny S., 2017, Biodiversity and phylogeny of Marasmius (Agaricales, Basidiomycota) from Madagascar, Phytotaxa 292 (2), pp. 101-149 : 144-145

35. Marasmius hinnuleus Berk. & M.A. Curtis, J. Linn. Soc., Bot. 10(45): 297. 1868 (1869). ( Fig. 23 , Plate 8 )

Type:— CUBA. On dead leaves, October, Wright 155 ( K).

Description:— Pileus 6–8 mm diam, campanulate to hemispherical, some with a small umbo; margin sulcate; surface dry, rugulose around disc, glabrous along margin; disc dark brown (6 E 6), ferruginous to brownish orange or reddish brown (6 E 7–D6–7) elsewhere. Context thin (<1 mm), white. Lamellae adnate, distant (15–17), no lamellulae, broad, not intervenose; cream to buff (4 A 2–3), edges ferruginous or non-marginate. Stipe 33–52 × 0.5 mm, central, cylindrical, wiry, hollow; surface glabrous; apex light brown (5D5), base dark brown (6 F 8). Odor and taste not distinctive.

Basidiospores 10.4–13.6 × 2.4–4 μm [x m = 12.13 ± 0.94 × 3.24 ± 0.49 μm; Q = 3–5; Q m = 3.82 ± 0.45, n = 25, s =1], fusoid to oblong or narrowly ellipsoid, smooth, hyaline, inamyloid, thin-walled. Basidia not observed. Basidioles 22.4–25.6 × 5.6–8 μm, clavate to fusoid, hyaline, inamyloid, thin-walled. Cheilocystidia of Siccus - type broom cells; main body 12–23.2 × 4.8–7.2 μm, clavate to cylindrical, hyaline, inamyloid, apically thick-walled; apical setulae 0.8– 7.2 × 0.8–1.6 μm, cylindrical to conical or irregular, seldom branched, hyaline, inamyloid, thick-walled. Pleurocystidia 36–47.2 × 7–10 μm, common, subcylindrical to fusoid, some mucronate, hyaline, inamyloid, refractive, thin-walled. Pileipellis mottled, a hymeniform layer of Siccus - type broom cells; main body 8–16.8 × 4–7.2 μm, clavate, seldom 2–3 lobed, hyaline, inamyloid, thick-walled; apical setulae 0.8–4.8 × 0.8–1.6, cylindrical to conical, light brown to brown, inamyloid, thick-walled. Pileus trama interwoven; hyphae, 2.4–8 μm diam, cylindrical, smooth, hyaline, dextrinoid, thin-walled. Lamellar trama regular; hyphae 2.4–8.8 μm diam, cylindrical to inflated, hyaline, dextrinoid, thin-walled. Stipe tissue monomitic; cortical hyphae 4.8–7.2 μm diam, parallel, cylindrical, hyaline, dextrinoid, thin-walled; medullary hyphae 4–7.2 μm, cylindrical, parallel, hyaline, dextrinoid, thin-walled. Caulocystidia absent. Clamp connections present.

Habit, habitat, and known distribution:—Solitary or in small clusters on bamboo debris in groomed park. Cuba, Guadeloupe, Madagascar.

Material examined:— MADAGASCAR.Region Analamanga,City of Antananarivo, Parc Botanique de Zoologique ( P. B. Z. T.), near the garden of Crops Wild and Relatives ( CWR), elev. 1270 m, GPS: 18˚ 55.530’ S, 47˚ 31.350’ E, 8 February 2014, J. E. Shay 217 ( TAN).

Notes:— Marasmius hinnuleus , described originally from Cuba, is characterized by a small (<10 mm diam), sulcate, brownish orange to reddish brown pileus, distant (15–17), non-marginate lamellae, a non-insititious, glabrous stipe lacking caulocystidia, refractive, often mucronate pleurocystidia 7–10 μm diam, Siccus - type broom cells, and growth on dead leaves. The Madagascan specimen matches quite closely material reported from the Caribbean ( Singer 1976, Pegler 1983). The species shows similarities to M. hypophaeus Berk. & M.A. Curtis , M. confertus Berk. & Broome , M. suthepensis , and M. ferrugineus (Berk.) Berk. & M.A. Curtis. Marasmius hypophaeus forms fewer lamellae (11–13) with brownish orange edges, a smaller stipe (23–35 mm long), larger basidiospores (x = 17.2 × 4.0 μm) and strangulate pleurocystidia ( Desjardin et al. 2000, Wannathes et al. 2009a). Marasmius confertus and M. suthepensis have nearly smooth pilei and more numerous lamellae with multiple series of lamellulae ( Antonín 2007, Wannathes et al. 2009a). Marasmius ferrugineus forms a paler pileus, has fewer lamellae (8–10) and larger basidiospores (x = 17.0 × 4.0 μm) ( Singer 1976, Desjardin et al. 2000). An ITS sequence of JES 217 ( KX 148988) places M. hinnuleus in a clade with M. hypophaeus and M. grandisetulosus with 89% BS and 1.0 PP support ( Fig. 1a ).

Figures

FIGURE 1a. Maximum likelihood phylogeny based on ITS sequence data. Marasmius from Madagascar are indicated in bold type.Values separated by / refer to nonparametric ML bootstrap proportions and Bayesian posterior probabilities, respectively. Values greater than 70/0.70 are shown (- designates a value below 70% or below 0.70). Nodes receiving support values greater than 90/0.95 are represented by bold branches. G—sect. Globulares; N—sect. Neosessiles; SA—sect. Sicci ser. Atrorubentes; SL—sect. Sicci ser. Leonini; SH—sect. Sicci ser. Haematocephali.

PLATE 8,Basidiocarps representingsect.Sicciser.Haematocephalia) Marasmius haematocephalus(JES 202) b)Marasmius ferruginoides (JES 209) c) Marasmius hinnuleus (JES 217). Scale bar = 10 mm. Photography by D.S. Newman.

FIGURE 23. Marasmius hinnuleus (JES 217) a) basidiospores; b) basidioles; c) cheilocystidia; d) pleurocystidia; e) pileipellis cells. Scale bar = 10 μm. Illustrated by J.E. Shay.