Anopheles pullus M. Yamada

Rueda, Leopoldo M., Brown, Tracy L., Kim, Heung-Chul, Klein, Terry A., Thongkukiatkul, Amporn & Sherwood, Van, 2009, Description and comparison of morphological structures of the eggs of Anopheles hyrcanus group and related species (Diptera: Culicidae) from the Republic of Korea, Zootaxa 2268, pp. 23-40 : 29

4. Anopheles pullus M. Yamada

( Fig. 3 D, 4D, 5D, 6D)

Size: Length 447.76–551.20 um (mean 497.04 + 28.83 um, n = 15); width 64.26–124.80 (mean 97.96 + 21.66 um, n = 12) (Table 1). Color: Black. Overall appearance: Slightly boat-shaped in both ventral and dorsal views, anterior end blunt, posterior end slightly pointed, sometimes blunt. Ventral surface slightly concave, dorsal surface curved, float relatively short and wide in dorso-ventral plane, length 244.95–499.72 um (mean 318.18 + 80.20, n = 14); width 35.70–87.36 um (mean 58.83 + 14.10 um, n = 14). Dorsal and lateral surfaces: All surfaces uniformly covered with quadrilateral, hexagonal, and mostly pentagonal outer chorionic cells or plastron-type cells. ( Fig. 3 D), each longer than wide, long dimension oriented in long axis of egg. Interior of each cell with perforated meshwork, surrounded by an elevated, palisade-like outer chorionic reticulum. Cell area 100.80–340.80 um (mean 236.64 + 64.82, n = 21) (Table 1). Float fairly short, about 0.57 length of egg; ratio of float length and width, and length in proportion to egg length and number of ribs as in Table 1. Ribs towards both ends of float wider than those at middle part, slightly striated on dorsal sides; number of ribs per float 19–27 (mean 24.38 + 2.03, n = 16). Ventral surface. Deck continuous, slightly narrows at middle of float, degree of narrowing usually variable; anterior part of deck usually as wide as posterior part; entire deck covered uniformly with fine tubercles ( Fig. 4 D). Frill continuous, shallow along narrowed portion of deck. Lobed ventral tubercles at anterior end of the deck, 4–8 (mean 6.36 + 1.36, n = 11), and at posterior end, 4–8 (mean 5.40 + 1.17, n = 10), (Table 1, Fig. 5 D). Lobed ventral tubercles usually round, occasionally oval or oblong. Lobes of each anterior ventral tubercle, 5–11 (mean 7.74 + 1.20, n = 43); lobes of each posterior ventral tubercle, 3–11 (mean 7.65 + 1.52, n = 31). Lobes clearly separated, often swollen at ends, outer walls often smooth. Lobes in slightly elevated, tuberculoid structures. Anterior end, micropyle. Anterior end slightly more blunt than posterior end. Micropylar collar irregular in outline, with smooth surface, inner edge uniformly and deeply excavated, peaks between excavations tapering to form radial ridges extending about half way across micropylar disc, dividing disc into sectors ( Fig. 6 D). Number of sectors (or ridges) 7–8 (mean 7.50 + 0.71, n = 2). Area of micropylar disc 66.61–109.59 um (mean 88.10 + 30.39 um, n = 2), usually with striated surface.

Figures

FIGURE 3. Anterior dorsal plastron of Anopheles (Anopheles) species. A, An. kleini; B, An. sinensis; C, An. sineroides; D, An. pullus; E, An. lindesayi japonicus; F, An. koreicus; G, An. lesteri; H, An. belenrae. Magnification, 3500 x.

FIGURE 4. Chronic tubercles of middle part of deck of Anopheles (Anopheles) species. A, An. kleini; B, An. sinensis; C, An. sineroides; D, An. pullus; E, An. lindesayi japonicus; F, An. koreicus; G, An. lesteri; H, An. belenrae. Magnification, 3500 x.

FIGURE 5. Anterior ventral tubercles of Anopheles (Anopheles) species. A, An. kleini; B, An. sinensis; C, An. sineroides; D, An. pullus; E, An. lindesayi japonicus; F, An. koreicus; G, An. lesteri; H, An. belenrae. Magnification, 3500 x.

FIGURE 6. Dorsal micropylar discs of Anopheles (Anopheles) species. A, An. kleini; B, An. sinensis; C, An. sineroides; D, An. pullus; E, An. lindesayi japonicus, F, An. koreicus, G, An. lesteri, H, An. belenrae. Magnification, 3500 x.