Knufia aspidiotus F. He & X. Z. Liu,

He, Fan, Lin, Bin, Sun, Jing-Zu & Liu, Xing-Zhong, 2013, Knufia aspidiotus sp. nov., a new black yeast from scale insects, Phytotaxa 153 (1), pp. 39-50: 43-44

publication ID

http://doi.org/ 10.11646/phytotaxa.153.1.2

persistent identifier

http://treatment.plazi.org/id/03AA87C5-5A69-FF83-FF01-FB88FE4FF808

treatment provided by

Felipe

scientific name

Knufia aspidiotus F. He & X. Z. Liu
status

sp. nov.

Knufia aspidiotus F. He & X. Z. Liu  , sp. nov. ( Fig. 2View FIGURE 2) MycoBank MB 805432

Differs from related Knufia species  mainly by its holoblastic conidia with less septa and being isolated from Aspidiotus sp. (scale insect).

Type: — China, Beijing: Yanqing, Song Mountain , on the branches of the buckthorn trees, Rhamnus parvifolia  , 18 August 2011, F  . He ( CGMCC3.15211View Materials, holotype!) 

Knufia aspidiotus  grows slowly on PDA, MEA, CMA and OA at 10, 20, 25, 30 and 37 ° C, and the optimum temperature is 25 ° C. Colonies on PDA reaching 13–16 mm diam, initially black, hard, later moist and cauliflower-like, and subcircular to irregular, raised, with even margin after 2–3 months at 25 ° C ( Fig. 1aView FIGURE 1 and 2aView FIGURE 2). Colonies on MEA black, moist, reaching 14–16 mm diam at 42 days at 25 ° C, with scarce aerial hyphae, furrows, circular to subcircular, raised, margin even ( Fig. 1bView FIGURE 1). Colonies on CMA brown to dark brown, effuse, velvety, with irregular margin, reaching 18–21 mm diam after 42 days at 25 ° C, and hyphae at margin submerged ( Fig. 1cView FIGURE 1). Colonies on OA pale gray to gray, effuse, velvety, circular to subcircular, with 25–26 mm diam at 42 days at 25 ° C, and have the appressed hyphae at margin ( Fig. 1dView FIGURE 1).

Mycelium consisting of smooth undifferentiated hyphae and moniliform hyphae, dark brown, catenulate, septate, branched at nearly right angles ( Fig. 2bView FIGURE 2). Undifferentiated hyphae formed by subspherical and cylindrical cell with thick wall, 3–5.5 × 8–13 µm ( Fig. 2e, f and hView FIGURE 2), and becoming moniliform and disarticulating into multicellular segments with age. Conidiogenous cell lateral or terminal, hyaline to subhyaline, borne on undifferentiated hyphae, forming the conidia chain and gradually moniliform hyphae. Yeast-like cell, ellipsoidal to spherical, producing several spherical, blastic conidia by multilateral budding ( Fig 2jView FIGURE 2). Conidia holoblastic, acropetal or sympodial, terminal or lateral, hyaline to subhyaline, smooth and thin-walled, ellipsoidal to pyriform, aseptate to multiseptate, 1.5–4 × 2.5–6 µm (aseptate), 4–7 × 12.5–15 µm (1-septate), 5–8 × 14–18 µm (2-septate) ( Fig. 2g and iView FIGURE 2). Endoconidia observed in some undifferentiated hyphae, subglobose, ellipsoidal to reniform, 2–4 × 3–4 µm ( Fig. 2dView FIGURE 2). The hyphal cell in old portions of colony swollen, forming darkly pigmented multicellular bodies, thick-walled, subglobose to globose, 7–12 × 9–12 µm, transversely, longitudinally and obliquely septate ( Fig 2cView FIGURE 2).

Etymology: — aspidiotus  refers that the scale insect, Aspidiotus sp., from which the species was isolated.

Distribution: —Song Mountain, Yanqing, Beijing, China.

Host: — Aspidiotus sp., Armoured scale insects ( Hemiptera  Diaspididae  ) living on the branches of the buckthorn trees, Rhamnus parvifolia  .

Other material studied: — China, Beijing: Yanqing, Song Mountain , on the branches of the buckthorn trees, Rhamnus parvifolia  , 18 August 2011, F.He ( CGMCC3.15246View Materials isotype!)  ; China, Beijing: Yanqing, Song Mountain , on the branches of the buckthorn trees, Rhamnus parvifolia  , 18 August 2011, F. He ( CGMCC3.15247View Materials isotype!)  .

F

Field Museum of Natural History, Botany Department

PDA

Royal Botanic Gardens

CMA

Crayford Manor House Adult Education Centre

C

University of Copenhagen

Kingdom

Fungi

Phylum

Ascomycota

Genus

Knufia