Pygmarrhopalites leonardwoodensis, Zeppelini, Douglas, Taylor, Steven J. & Slay, Michael E., 2009

Zeppelini, Douglas, Taylor, Steven J. & Slay, Michael E., 2009, Cave Pygmarrhopalites Vargovitsh, 2009 (Collembola, Symphypleona, Arrhopalitidae) in United States, Zootaxa 2204, pp. 1-18 : 3-5

Pygmarrhopalites leonardwoodensis sp. nov.

pygmaeus -group s. str.; Fig. 1

Description. Animals white in alcohol. Dorsal body setae short on anterior and posterior part of the great abdomen, the posterior setae much longer than the anterior ( Fig. 1 A), posterior setae 0.56 times length of third unguis ( Table 2 ). Ant. IV of holotype as long as cephalic diagonal, with five subsegments, apex with capitate sense rod ( Fig. 1 B). Ant. III not swollen basally; sense organ ( Fig. 1 C) with 2 sense rods in separate sockets; seta Aai club-shaped, blunt; Api and Ape slender, acuminate; Ae, Ap and Ai normal, elongate setae. 1+1 eyes. Dorsal cephalic setae not spine-like, M5 absent ( Fig. 1 D). Metatrochanteral organ (seta D2) elongate ( Fig. 1 E). Seta FSa present on all tibiotarsi. All ungues with inner tooth, no tunica. All unguiculi with corner tooth, apical filament reaching unguis tip of first and second claw pairs ( Fig. 1 F). Corpus tenaculum with two setae ( Fig. 1 G). Dens with 7 dorsal E setae, E1 and E3 strongly spine-like, other E setae normal; L1–3 strongly spine-like, 4 ventral setae rows (3,2,1,1) present ( Fig. 1 H), dental chaetotaxy in Table 3. Mucro narrow, gutter-like, with narrow tip, both edges serrate ( Fig. 1 I). Anal valve without cuticular spines ( Fig. 1 J); setae C1 swollen, C3–4 slightly lamellate, C5–6 lamellate, C6 branched at base in all specimens examined, D5 present, D7 absent, chaetotaxy in Table 4 . Female subanal appendage unilaterally fringed in distal half ( Fig. 1 K).

Type material. Holotype (female) (number 2 in the slide): USA, MISSOURI, Pulaski Co., Folly Cave, 12-iv-04, S. Taylor, M. Slay leg. ( INHS). Paratypes: female, in the same slide with holotype and 5 paratypes in alcohol: USA, MISSOURI, Pulaski Co., Folly Cave, 12-iv-04, S. Taylor, M. Slay leg. ( INHS).

Etymology. Refers to the military installation, Fort Leonard Wood, where the species is known to occur.

Remarks. Pygmarrhopalites leonardwoodensis was found in a single cave in Fort Leonard Wood, a military installation at the physiographic province Salem Plateau of the Ozark Plateaus. The climate is Cfa, temperate/mesothermal, humid subtropical with uniform precipitation distribution.

This species resembles P. madonnensis from Illinois in the foot complexes, head and apical organ of the third antennal segment chaetotaxies, and fourth antennal segment subsegmentation. The strongly serrated lamellae on C4–6 of P. madonnensis, and the short posterior body setae, the female subanal appendages, the presence of E7 and the L3 strongly spine-like on dens in P. leonardwoodensis can differentiate the species from each other.

Tibiotarsus 2 width 29.78 24.17 38.47 38.35 25.68 25.57

continued next page 1Abbreviations in parentheses are used in the table above: Antennae and head: Apical subsegment of the fourth antennal segment (apic-antIV), second subsegment of the fourth antennal segment (antIV sub seg 2), third subsegment (antIV sub seg 3), fourth subsegment (antIV sub seg 4), fifth subsegment (antIV sub seg 5), sixth subsegment (antIV sub seg 6), basal subsegment of the fourth antennal segment (bas-antIV), total length of the fourth antennal segment (Total AntIV), longest seta of the segment (Longest Seta AntIV), widest subsegment of the fourth antennal segment (Widest Subseg AntIV), ratio of the longest seta and the widest subsegment width (ratio Lseta/Wsubseg), ratio of antIV total length and cephalic diagonal (Ratio AntIV/cep-diag), cephalic diagonal (cep-diag), ratio of apical subsegment and total length of fourth antennal segment (ratio Apical subseg / total AntIV), ratio of basal subsegment and total length of fourth antennal segment (ratio Basal subseg /total AntIV); Body: length of the posterior body seta (post-bd-st), maximum length of the third unguis (ung-III), ratio of posterior body seta and the third unguis length (ratio post-bd-st/ung-III), Total Body Length; Legs: length and width of coxa, trochanter, femur and tibia of all legs, ratio of length and width for each leg segment; Dens: dens length, dens width, ratio of length and width of dens (Length/Width Ratio-Dens).

Figures

FIGURE 1. Pygmarrhopalites leonardwoodensis sp. nov.: A, Dorsal body seta (a- anterior part of the abdomen, bposterior part of the abdomen); B, Fourth antennal segment; C, Apical sensory organ of the third antennal segment; D, Posterior dorsal cephalic chaetotaxy; E, Metatrochanteral organ; F, Empodial complex (a –– first leg, b –– second leg, c –third leg); G, Tenaculum; H, Dorsal and ventral chaetotaxy of the dens; I, Mucro with narrow tip; J, Chaetotaxy of the anal valve; K, Subanal appendage of the female.

Tables

TABLE 2. Measurements and ratios for structures of the holotypes of the six new species of Pygmarrhopalites. All measurements in micrometers.

Structure or Ratio1	P. ashcraftensis	P. leonardwoodensis	P. buffaloensis	P. youngsteadtii	P. plethorasari	P. shoshoneiensis
Antennae and head
apic-antIV antIV sub seg 2 antIV sub seg 3 antIV sub seg 4	68.86 42.93 31.48 42.02	69.55 27.01 31.19 31.36	- - - -	108.21 50.34 50.21 62.84	79.84 26.23 28.40 28.26	59.49 20.29 27.16 31.07
antIV sub seg 5 antIV sub seg 6 bas-antIV Total AntIV	58.26 - 319.38 562.95	- - 134.96 294.09	- - - -	77.92 78.94 356.74 785.23	- - 137.76 300.52	30.10 31.42 130.37 329.93
Longest Seta AntIV Widest Subseg AntIV ratio Lseta/Wsubseg Ratio AntIV/cep-diag	87.01 24.06 3.61 1.80	51.01 19.80 2.57 1.00	- - - -	132.46 32.46 4.08 1.56	67.08 17.83 3.76 1.32	92.81 21.13 4.39 1.34
cep-diag ratio Apical subseg/total AntIV	312.68 0.12	294.06 0.23	- -	502.93 0.13	227.23 0.26	244.53 0.18
ratio Basal subseg/total AntIV	0.56	0.45	-	0.45	0.45	0.39
Body post-bd-st ung-III Ratio post-bd-st/ung-III Total Body Length	99.51 74.39 1.33 1071.67	29.31 52.11 0.56 1048.61	102.49 83.24 1.23 1052.47	119.52 111.81 1.06 1617.66	58.44 56.56 1.03 820.76	86.61 62.81 1.37 1244.59
Legs Coxa 1 length Trochanter 1 length Femur 1 length	- 83.31 188.73	57.45 58.99 113.98	57.35 68.88 137.66	114.34 137.39 269.29	43.49 61.85 115.83	62.94 54.25 110.37
Tibiotarsus 1 length Coxa 1 width Trochanter 1 width Femur 1 width	332.19 - 36.16 34.99	168.29 30.88 28.73 43.27	281.11 41.12 34.06 55.55	475.49 84.11 68.62 67.89	239.45 29.78 29.65 35.14	210.1 47.07 48.93 36.39
Tibiotarsus 1 width Coxa 2 length Trochanter 2 length Femur 2 length	33.74 62.45 120.36 189.19	25.63 45.85 57.30 120.1	33.64 55.04 72.94 165.55	57.86 - - 340.4	26.11 58.81 58.24 136.33	32.73 56.98 75.65 134.6
Tibiotarsus 2 length Coxa 2 width Trochanter 2 width Femur 2 width	372.86 55.48 36.23 34.19	231.69 36.01 33.56 41.48	290.21 53.44 54.09 53.84	505.95 - - 87.47	247.66 26.75 26.44 41.21	240.38 55.65 30.09 42.07