Xiphinema vallense, Archidona-Yuste & Navas-Cortés & Cantalapiedra-Navarrete & Palomares-Rius & Castillo, 2016

Archidona-Yuste, Antonio, Navas-Cortés, Juan A., Cantalapiedra-Navarrete, Carolina, Palomares-Rius, Juan E. & Castillo, Pablo, 2016, Cryptic diversity and species delimitation in the Xiphinema americanum-group complex (Nematoda: Longidoridae) as inferred from morphometrics and molecular markers, Zoological Journal of the Linnean Society 176 (2), pp. 231-265 : 238-239

XIPHINEMA VALLENSE SP. NOV. ( FIGS 1 , 2 , 4 , 5 ;

TABLES 3 AND 4)

Holotype: Female extracted from a loam soil from the rhizosphere of wild olive ( Olea europaea ssp. sylvestris ) in San José del Valle, Cádiz province, southern Spain (36°37′57.30″N, 5°46′20.00″W) by J. Martín Barbarroja and G. León Ropero, mounted in pure glycerine, and deposited in the nematode collection at IAS-CSIC (collection number AR55-06).

Paratypes: Female, male, and juvenile paratypes extracted from the rhizosphere of wild olive ( Olea europaea ssp. sylvestris ) in San José del Valle , Cádiz province , southern Spain. Additional populations collected in Bolonia , Cádiz province , and Hinojos, Huelva province, both in southern Spain, associated with wild and cultivated olive, respectively, were deposited in the fol-

lowing nematode collections: IAS-CSIC (collection numbers AR55-01–AR55-05, AR55-07–AR55-011); two female paratypes deposited at the Royal Belgian Institute of Natural Sciences , Brussels, Belgium ( RIT830 ) ;

Figures

Figure 1. Line drawings of: A–D, Xiphinema plesiopachtaicum sp. nov.; E–H, Xiphinema vallense sp. nov.; I–L, Xiphinema astaregiense sp. nov. A, E, I, female lip regions. B–D, F, G, J, K, female tail regions. H, L, male tail regions.

Figure 2. Line drawings of pharyngeal bulb and anterior genital branch of: A, B, Xiphinema plesiopachtaicum sp. nov.; C, D, Xiphinema vallense sp. nov.; E, F, Xiphinema astaregiense sp. nov.

Figure 4. Light micrographs of Xiphinema vallense sp. nov. A, entire female. B, E, female neck region. C, D, F, female lip region. G, vulval region. H–M, female tail regions from different specimens showing the morphological variability. N–O, male tail, ventromedian supplements arrowed. Abbreviations: a, anus; gr, guiding ring; V, vulva. Scale bars: A = 200 μm; B–O = 20 μm.

Figure 5. Relationship between body length and functional and replacement odontostyle (Ost and rOst, respectively) length in all developmental stages from first-stage juveniles (J1) to mature females of: A, Xiphinema vallense sp. nov. and B, Xiphinema astaregiense sp. nov.