Rhizoctonia spp

Piesik, Dariusz, Lemanczyk, Grzegorz, Bocianowski, Jan, Buszewski, Bogusław, Vidal, Stefan & Mayhew, Chris A., 2022, Induction of volatile organic compounds in Triticum aestivum (wheat) plants following infection by different Rhizoctonia pathogens is species specific, Phytochemistry (113162) 198, pp. 1-9 : 5

4.2. Rhizoctonia spp . Infestation

All wheat plants used for the experiments were at the BBCH 33 stage ( Lancashire et al., 1991), were infected by one of four Rhizoctonia species [ R. cerealis (AG-D I), R. solani (AG 5), R. zeae (WAG-Z), and R. oryzae (WAG-O)] ( Table 1). R. zeae and R. oryzae were provided by Filiz Unal, Ministry of Agriculture and Rural Affairs, Central Plant Protection Research Institute, 06172, Yenimahalle, Ankara, Turkey, whereas the other two species were isolated from winter wheat by one co-author and characterized by PCR and ITS sequencing. For soil inoculation, we used wheat grains (cv. ‘Jenga’) colonized by the Rhizoctonia species. The soil was inoculated with the pathogen inoculum during sowing. The inoculum was applied 1 cm above planted grains at an amount of 1.4 g per pot. Shoots were inoculated by placing 3 wheat (to stem base) seeds infected with the respective Rhizoctonia species on the stem base of wheat seedlings at growth stage BBCH 33.