Pseudomonas aeruginosa (Schroeter, 1872) Migula, 1900

Kahraman, H. & Karaderi, C. C., 2022, Production of proline and protease with different organic wastes in bacteria (Production proline and protease with organic wastes), Brazilian Journal of Biology (e 243187) 82, pp. 1-8 : 2-3

P. aeruginosa was cultivated in Luria- Bertani (LB) broth at 37 °C, static on incubator for overnight (O/N).

100μl of overnight cultures was setting OD 600 nm of 0.2- 0.3 grown tube and filled with 5 ml in 10 ml tubes was inoculated, and incubated for 24 h of time. These cultures were subsequently incubated on 37 °C for 24 h. Bacterial growth was determined by measuring the absorbance at 600 nm (OD

600) by a visible spectrophotometer.