Fusarium incarnatum-equiseti

Maryani, N., Sandoval-Denis, M., Lombard, L., Crous, P. W. & Kema, G. H. J., 2019, New endemic Fusarium species hitch-hiking with pathogenic Fusarium strains causing Panama disease in small-holder banana plots in Indonesia, Persoonia 43, pp. 48-69 : 58

Fusarium incarnatum-equiseti species complex (FIESC) phylogeny

The 11 isolates belonging to the FIESC were assessed using a more inclusive analysis based on five loci (cmdA, ITS, LSU, rpb2 and tef1; Fig. 3 ). The alignment consisted of a total 2 746 char- acters (cmdA 653, ITS 510, LSU 562, rpb2 597 and tef1 424), from 93 isolates, including all the phylogenetic clades known in this species complex plus two outgroup taxa ( Fusarium circinatum NRRL 25331 and F. fujikuroi NRRL 13566). Multi-gene phy- logenetic inference was able to recognise six new phylogenetic species in the FIESC. The number of new phylogenetic species recognised is equally distributed in the incarnatum clade and the equiseti clade (three new phylospecies each) sensu O’Donnell et al. (2009). In the incarnatum clade, isolates InaCC F940, InaCC F941, Indo167, InaCC F964, Indo186, and Indo188 clustered in a distinct clade (55 bp/0.99 pp) closely related to the phylogenetic species FIESC-16 which is introduced here as phylogenetic species FIESC-32. These isolates were obtained from five different banana variety hosts in Sulawesi and Kalimantan. The other two new species in the incarnatum clade are monotypic lineages represented by isolate Indo161 (99 bp/1 pp) closely related to FIESC-26 and isolate InaCC F965 (50 bp/ 1 pp) closely related to FIESC-24, introduced as phylogenetic species FIESC-33 and FIESC-34, respectively. In the equiseti clade, three isolates: Indo174 (99 bp/1 pp) closely related to FIESC-1; Indo175 (-/1 pp) and InaCC F963 (55 bp/1 pp), both isolates closely related to FIESC-13, formed monotypic lineages which are introduced here as FIESC-29, FIESC-30, and FIESC-31, respectively. These phylogenetic species were isolated from two banana varieties in relatively close proximity in South Kalimantan.

Figures

Fig. 3 Maximum likelihood tree inferred from the combined cmdA, ITS, rpb2, tef1, and LSU sequence datasets of the Fusarium incarnatum-equiseti species complex (FIESC) including 11 Indonesian isolates (indicated in blue). Bootstrap support values and Bayesian posterior probabilities are given at each node. The tree is rooted to Fusarium circinatum (NRRL 25331) and Fusarium fujikuroi (NRRL 13566).