Fusarium fujikuroi

Maryani, N., Sandoval-Denis, M., Lombard, L., Crous, P. W. & Kema, G. H. J., 2019, New endemic Fusarium species hitch-hiking with pathogenic Fusarium strains causing Panama disease in small-holder banana plots in Indonesia, Persoonia 43, pp. 48-69 : 57-58

Fusarium fujikuroi species complex (FFSC) phylogeny

The eight isolates belonging to the FFSC were further analysed using a multi-gene phylogeny based on cmdA, rpb1, rpb2, tef1, and tub. The final alignment included 4 795 characters (cmdA 545, rpb1 1534, rpb2 1551, tef 677 and tub 488) including align- ment gaps, and encompassed 54 isolates, with two outgroup taxa ( F. oxysporum CBS 716.74 and CBS 744.97) ( Table 2 ).

The analysis was consistently able to distinguish the three biogeographical clades known as the African,American and Asian clades sensu O’Donnell et al. (1998 a). All of the Indonesian isolates clustered within the Asian clade of FFSC except for isolate InaCC F991, identified as F. verticilloides , and clustered within the African clade ( Fig. 2 ). According to the multi-gene analysis, two isolates (InaCC F962 and InaCC F992) were identified as F. proliferatum , while two new phylogenetic species were recognised among the Indonesian isolates. Isolates InaCC F872 and InaCC F993, from central and East Java, respectively, clustered in a distinct, highly supported clade (96 bs/0.99 pp) closely related to F. mangiferae . Isolates InaCC F950–152, formed a distinct group (100 bs/1.0 pp), closely related to, but genetically distinct from F. sacchari .

Figures

Fig. 2 Maximum likelihood tree inferred from the combined cmdA, tef1, tub, rpb1, and rpb2 sequence datasets of the Fusarium fujikuroi species complex (FFSC) including eight Indonesian isolates (indicated in blue). Bootstrap support values and Bayesian posterior probabilities are given at each node. The tree is rooted to Fusarium nirenbergiae (CBS 744.97) and F. oxysporum (CBS 716.74).