Fusarium longipes Wollenw. & Reinking, 1925

Maryani, N., Sandoval-Denis, M., Lombard, L., Crous, P. W. & Kema, G. H. J., 2019, New endemic Fusarium species hitch-hiking with pathogenic Fusarium strains causing Panama disease in small-holder banana plots in Indonesia, Persoonia 43, pp. 48-69 : 65-66

Fusarium longipes Wollenw. & Reinking ,

Phytopathology 15: 160. 1925

— Fig. 11

Sporulation abundant from conidiophores carried on aerial mycelium and from sporodochia. Conidiophores on aerial mycelium abundant on PDA and SNA, rare on CLA, septate, branch- ing irregularly, mostly reduced to solitary conidiogenous cells formed singly and laterally on aerial hyphae; conidiogenous cells monophialidic, doliiform to ampulliform, smooth- and thin-walled, (7–)10–13(–15) × 3–4(–5) µm (av. 12 × 6 µm), formed laterally on aerial hyphae or clustering terminally on conidiophores, with a minute collarette; conidia (microconidia) obovoid to ellipsoid, rough- and thin-walled, (7–)10–19(–23) × (3–)4(–5) μm (av. 15 × 4 µm), 0–2-septate, arranged in false heads on monophialides. Sporodochia formed abundantly on CLA after 7 d, bright orange, later turning red to purple; conidiophores in sporodochia highly irregularly or verticillately branched, sympodially to solitary conidiogenous cells; conidiogenous cells monophialidic, doliiform, ampulliform to subcylindrical, 7–11(–14) × (2–)2.5–3.5(–4) µm (av. 9.5 × 3 µm), with inconspicuous collarets; sporodochial conidia falcate, apical cells strongly curved, tapering and whip-like with rounded apex, basal cells foot-shaped and elongated, (3–)4–5-septate: 3-septate conidia, 28.5 × 3.5 µm; 4-septate conidia, (37–)38–43 (–43.5) × 4.5–5.5 µm; 5-septate conidia, (37–)42–49.5(–53.5) × (3.5–)4.5–5(–6) µm; av. (28.5–)40.5–49.5(–53.5) × (3–)4– 5(–6) µm. Chlamydospores ellipsoid, sub-globose to globose, formed intercalary or terminal, single or in pairs, or in clumps, (7–)10–13(–15) × (7–)9–13(–14) µm (av. 12 × 11 µm), brown, rough-walled.

Culture characteristics — Colony on PDA showing optimal growth at 25 °C with an average growth rate of 4.2–4.9 mm /d. Colony reverse livid red becoming white towards the margin, becoming completely livid red to bay with age. Colony surface cottony greyish rose becoming vinaceous with age and white toward the margins. Aerial mycelium abundant, cottony, with high sporulation and lacking exudates. Sporodochia formed abundantly on CLA after 7 d, pale orange to orange.

Geography & Host — Katingan, Central Kalimantan, Musa sp. var. Pisang Awak (ABB).

Pathogenicity — Non-pathogenic on Cavendish (AAA).

Material examined. INDONESIA, Desa Tewang Menyangen, T . Sangalang, Katingan , Central Kalimantan ( E 113°6'552" S 1°41'83"), on infected pseudostem of Musa var. Pisang Awak ( ABB), 23 June 2014, N. Maryani (specimen and culture, InaCC F 974 , preserved in metabolically inactive state) .

Notes — This banana isolate of F. longipes displays some unique characteristics which differ slightly from F. longipes vide Leslie & Summerell (2006) , which include the presence of microconidia and chlamydospores. This species is more similar to F. equiseti as described by Nelson et al. (1983), except for the length of the long curvature of the macroconidia. Additionally, the chlamydospore formation also differs from the original description of F. longipes .

Figures

Fig. 11 Fusarium longipes (InaCC F974). a. Culture grown on PDA; b–c. sporodochia on carnation leaves; d. sporodochial conidiophores; e–f. branched conidiophores; g. falcate-shaped macroconidia; h. microconidia; i. chlamydospores. — Scale bars: b–k = 10 µm.