Fusarium kotabaruense N. Maryani, Sand.

Maryani, N., Sandoval-Denis, M., Lombard, L., Crous, P. W. & Kema, G. H. J., 2019, New endemic Fusarium species hitch-hiking with pathogenic Fusarium strains causing Panama disease in small-holder banana plots in Indonesia, Persoonia 43, pp. 48-69 : 65

Fusarium kotabaruense N. Maryani, Sand. -Den., L. Lombard, Kema & Crous, sp. nov

. — MycoBank MB828964;

Fig. 10

Etymology. Name refers to Kota Baru one of the nine regencies in the

Indonesian province of South Kalimantan.

Typus. INDONESIA, Desa Sungai Birah, Kecamatan Pamukan Barat, Kota Baru , Kalimantan Selatan ( E 115°59'982" S 2°22'883"), on infected pseudostem of Musa var. Pisang Hawa ( ABB), 19 June 2014, N. Maryani (holotype specimen and culture, InaCC F 963 , preserved in metabolically inactive state) .

Sporulation abundant from conidiophores carried on aerial mycelium. Conidiophores on aerial mycelium abundant on PDA and SNA, less frequent on CLA, septate, irregularly branching; conidiogenous cells mono- or polyphialidic, subulate to subcylindrical, smooth- and thin-walled, (15–)19–33(–40) × 4–7 µm (av. 26 × 5 µm), forming terminally, singly or in verticillately branched conidiophores, less commonly laterally or intercalary, proliferating percurrently, periclinal thickening inconspicuous or absent; falcate and multiseptate, apical cells papillate, basal cells indistinct or foot-shaped, (2–)3–5(–7)-septate, formed on both mono- and polyphialides: 2-septate conidia, (21–)21.5– 25(–26) × 5–6 µm; 3-septate conidia, (24.5–)28–35(–36.5) × 5.5–6.5(–7) µm; 4-septate conidia, (32–)34–39.5(–41.5) × 5.5–6.5(–7) µm; 5-septate conidia, (34.5–)36–42.5(–45) × (5–)5.5–6.5(–7.5) µm; 6-septate conidia, 39–40.5 × 5.5–7 µm; 7-septate conidia, (38.5–)39.5–44(–45) × 6–7 µm; av. (21–)31.5–41.5(–45) × (5–)5.5–6.5(–7.5) µm. Sporodochia and chlamydospores not observed.

Culture characteristics — Colony on PDA showing optimal growth at 25 °C with an average growth rate of 5.0– 6.85 mm /d. Colony reverse rosy buff. Colony surface cottony rosy buff. Aerial mycelium abundant, cottony, with high sporulation and lacking exudates.

Geography & Host — Kota Baru, South Kalimantan, Musa sp. var. Pisang Hawa (ABB).

Pathogenicity — Non-pathogenic on Cavendish (AAA).

Notes — Fusarium kotabaruense represents a species in the Equiseti clade of the FIESC and relatively fast growing (5.0– 6.85 mm /d). Most distinguishing characteristic of this spe- cies is the absence of sporodochia on CLA culture. However, aerial conidiophores are abundant with conidia produced with high variability in its septation, (0–)3–5(–7)-septate.

Figures

Fig. 10 Fusarium kotabaruense (ex-type InaCC F963). a. Culture grown on PDA; b. mycelium on carnation leaves; c–h. conidiophores and conidiogenous cells; i–k. conidia. — Scale bars: b = 200 µm; c–d = 50 µm; e–f, h–k = 10 µm; g = 5 µm.