Neoechinorhynchus (Neoechinorhynchus) schmidti Barger, Thatcher & Nickol, 2004, Barger, Thatcher & Nickol, 2004

Pinacho-Pinacho, Carlos Daniel, Sereno-Uribe, Ana L., León, Gerardo Pérez-Ponce De & García-Varela, Martín, 2015, Checklist of the species of Neoechinorhynchus (Acanthocephala: Neoechinorhynchidae) in fishes and turtles in Middle-America, and their delimitation based on sequences of the 28 S rDNA, Zootaxa 3985 (1), pp. 98-116: 106-111

publication ID

http://dx.doi.org/10.11646/zootaxa.3985.1.5

publication LSID

lsid:zoobank.org:pub:E6FF2FAB-4C99-4E4A-8E56-8D9B5A161474

DOI

http://doi.org/10.5281/zenodo.5685263

persistent identifier

http://treatment.plazi.org/id/03919446-652F-434E-8CE0-F8FFFEA2C3A7

treatment provided by

Plazi

scientific name

Neoechinorhynchus (Neoechinorhynchus) schmidti Barger, Thatcher & Nickol, 2004
status

 

Neoechinorhynchus (Neoechinorhynchus) schmidti Barger, Thatcher & Nickol, 2004  

( Fig. 2 View FIGURE 2 i)

Mexico: TABASCO: 24 km to S of Villahermosa; Trachemys venusta   (Barger 2004; Barger et al. 2004). Pantanos de Centla (18 ° 28 ’ 18.9 ’’N 92 ° 39 ’ 14.9 ’’W). Trachemys scripta   ( Martínez-Aquino et al. 2009; García-Varela et al. 2011).

Specimens deposited. CNHE (6764), HWML (17667), USNPC (92883).

Species delimitation through phylogenetic analysis and genetic divergence. To demonstrate the species boundaries among the species reported in this checklist, we sequenced a total of 269 individuals of the species: N. (N.) brentnickoli   (81 individuals from 16 localities); N. (N.) roseum   (two individuals from two localities); N. (N.) mamesi   (22 individuals from seven localities); N. (N.) golvani   (80 individuals from 11 localities); N. (N.) mexicoensis   (47 individuals from eight localities); N. (N.) schmidti   (five individuals from one locality); N. (N.) emyditoides   (26 individuals from three localities); N. (N.) panucensis   (four individuals from two localities); and N. (N.) chimalapasensis   (two individuals from one locality). Additionally sequence of N. saginata   plus Floridosentis mugilis   and Floridosentis pacifica   were aligned together, composing a data set of 806 bp with 274 terminals. Nucleotide frequencies for 28 S rDNA data set were 0.246 (A), 0.178 (C), 0.276 (G), and 0.298 (T). Phylogenetic analyses of the available sequences for the genus Neoechinorhynchus   through the Bayesian consensus tree yielded the same branch pattern as the ML tree ( Fig. 3 View FIGURE 3 ). Both trees yielded that the ten species of Neoechinorhynchus   are monophyletic with strong nodal support ranging from 72 to 100 % of bootstrap, and 1.0 of posterior probability ( Fig. 3 View FIGURE 3 ). Both trees yielded three major clades. The first clade contained four species N. (N.) roseum   , N. saginata   , N. (N.) schmidti   and N. (N.) emyditoides   . The second clade was composed of two species N. (N.) golvani   and N. (N.) panucensis   and had 100 % bootstrap support and 1.0 posterior probability. The third clade was composed of four species N. (N.) chimalapasensis   , N. (N.) brentnickoli   , N. (N.) mamesi   and N. (N.) mexicoensis   and received reliable bootstrap support and high values of posterior probability ( Fig. 3 View FIGURE 3 ).

67- 76 N. (N.) brentnickoli   10 D. latifrons Laguna   de Tres Palos, Guerrero, México FJ388991 View Materials , 8178 FJ 968156 View Materials - FJ 968159 View Materials , KR086219 View Materials - KR086223 View Materials *

77- 81 N. (N.) brentnickoli   5 D. latifrons Río Tamarindo, Chautengo   , Guerrero, KR086224 View Materials - KR086228 View Materials * 8179 México

82 N.   (N.) roseum 1 Achirus mazatlanus Laguna   el Caimanero, Sinaloa, México FJ 388999 View Materials 6762, 633, 634 83 N.   (N.) roseum 1 Citharichthys gilberti Estero La Tovara   , Nayarit, México FJ 389000 View Materials 6763 84- 86 N. (N.) mamesi   3 D. latifrons Puente Manialtepec   , Oaxaca KR086229 View Materials - KR086231 View Materials *

......continued on the next page TABLE 1 View TABLE 1 . (Continued)

Samples Species N Host Locality GenBank Specimensdeposited

( DNA) CNHE

87- 91 N. (N.) mamesi   5 D. latifrons Estero Joaquin Amaro   , Chiapas, México JN 830770 View Materials - JN 830774 View Materials 8183

92- 95 N. (N.) mamesi   4 D. latifrons Laguna   la Conquista, Chiapas, México JN 830766 View Materials - JN 830769 View Materials 8184

96- 98 N. (N.) mamesi   3 D. latifrons Laguna Rion Pijijiapan   , Chiapas, JN 830763 View Materials - JN 830765 View Materials 8180, 8181, 8182

México

99- 100 N. (N.) mamesi   2 D. latifrons Quebrada Ganados   , Costa Rica KR086232 View Materials *, KR086233 View Materials * 8590

101- 102 N. (N.) mamesi   2 D. maculatus Playa Grande   , Costa Rica KR086234 View Materials *, 8191 KR086235 View Materials *

103- 105 N. (N.) mamesi   3 D. latifrons Las   lisas, Guatemala KR086236 View Materials - KR086238 View Materials *

106- 107 N. (N.) golvani   1 Parachromis Lago   Jalapa, Costa Rica KR086239 View Materials *

managuensis  

108- 109 N. (N.) golvani   2 Parachromis loisellei Lago   Jalapa, Costa Rica KR086240 View Materials *,

KR086241 View Materials *

110- 111 N. (N.) golvani   2 Amphilophus Lago   Jalapa, Costa Rica KR086242 View Materials *, KR086243 View Materials * 8592 longimanus

112 N.   (N.) golvani 1 Heterotilapia Lago   Jalapa, Costa Rica KR086244 View Materials *

multiespinosa  

113- 114 N. (N.) golvani   2 Archocentrus Lago   Jalapa, Costa Rica KR086245 View Materials *,

centrarchus   KR086246 View Materials *

115- 116 N. (N.) golvani   2 Amatitlania Quebrada Puercos   , Costa Rica FJ388998 View Materials , 6757 nigrofasciata   KR086247 View Materials *

117- 127 N. (N.) golvani   11 Vieja pearsei Presa Chicoasen   , Chiapas, México FJ 388995 View Materials , FJ 968136 View Materials - FJ968138 View Materials , 6755 KR086248 View Materials - KR086254 View Materials *

128- 132 N. (N.) golvani   5 V.   pearsei Presa Nezahualcoyolt, Chiapas, México FJ 388996 View Materials , FJ 968141 View Materials , FJ968142 View Materials , 6756 KR086255 View Materials *,

KR086256 View Materials *

133- 143 N. (N.) golvani   11 Parachromis Lago Canitzan, Tenosique   , Tabasco, FJ 388994 View Materials , FJ 968139 View Materials , FJ968140 View Materials , 6767 friedrichstalii   México KR086257 View Materials - KR086264 View Materials *

144- 153 N. (N.) golvani   10 Cichlasoma Río Carrizal   , Tabasco, México FJ 388993 View Materials , FJ 968134 View Materials , FJ968135 View Materials , 6754 urophthalmum   KR086265 View Materials - KR086271 View Materials *

154- 156 N. (N.) golvani   3 C. urophthalmum Lago   las Ilusiones, Tabasco, México FJ 388992 View Materials - FJ 968144 View Materials

157- 167 N. (N.) golvani   11 Paraneetroplus Lago   de Catemaco, Veracruz, México FJ 388986 View Materials , FJ 968145 View Materials , FJ 968146 View Materials , 601, 603, 604, 606, 631,

fenestratus   KR086272 View Materials - KR086279 View Materials * 632, 6783

168- 172 N. (N.) golvani   5 C. urophthalmum Presa Temascal   , Oaxaca, México KR086280 View Materials - KR086284 View Materials * 8593

......continued on the next page TABLE 1 View TABLE 1 . (Continued) The sequence divergence estimated among the nine species herein considered, plus an additional sequence available for a species that occurs in freshwater fishes in North America ( N. saginata   ) ranged from 3.2 % to 48.7 % ( Table 2). Instead, the intraspecific genetic divergence varied markedly among all the isolates sequenced for each acanthocephalan species and it was never higher than 3 % for all intraspecific comparisons. For instance, the divergence among 81 specimens of N. (N.) brentnickoli   obtained from a wide geographic range, in 16 localities (localities 1-16 in Fig. 1 View FIGURE 1 ) across the Pacific coast of northwestern Mexico, varied from 0 to 0.7 % ( Table 2). In other example, N. (N.) roseum   corresponding to two individuals of two localities 1) Laguna el Caimanero, Sinaloa and 2) Estero La Tovara, Nayarit (localities 17-18), had genetic divergence from 0 to 0.04 %. The species N. (N.) mamesi   comprises 22 individuals from seven localities from Mexico, one of Guatemala and two from Costa Rica (localities 19-25 in Fig. 1 View FIGURE 1 ), showing a genetic divergence from 0 to 1.3 %. N. (N.) golvani   shows genetic divergence from 0 to 1.5 % among 80 specimens from 11 localities (see localities 26-36). The species N. (N.) mexicoensis   corresponding to 47 individuals from eight localities (localities 37-44) from Gulf of Mexico shows a genetic divergence ranged from 0 to 3 %. N. (N.) schmidti   shows low genetic divergence ranged from 0-0.02 % of specimens analyzed from the same locality. The species N. (N.) emyditoides   corresponding three localities: 1) Lago de Catemaco, Veracruz, 2) Río Papaloapan, Tlacotalpan, Veracruz and 3) Presa la Herradura, Monterrey (localities 46-48), showed a genetic divergence from 0 to1.3 %. The specimens of N. (N.) panucensis   are from Axtlan de Terrazas, San Luis Potosí, and Río Pantepec, Veracruz (localities 49-50), both localities are near of the type locality of N. (N.) panucensis   . The genetic divergence among four isolates of N. (N.) panucensis   ranged from 0.02–0.05 %. Finally, the two specimens of N. (N.) chimalapasensis   showed a genetic divergence equal to zero (locality 51).

1 2 3 4 5

1. N. (N.) brentnickoli   (n = 81) 0–0.7 42–43 8.1–9.8 21.9–23.7 7.3–10 2. N. (N.) roseum   (n = 2) 0–0.04 40.9–42.9 41.1–43.1 39.7–43.2 3. N. (N.) mamesi   (n = 22) 0–1.3 21–23.6 2.40–5.50 4. N. (N.) golvani   (n = 80) 0–1.5 21.0– 24.6 5. N. (N.) mexicoensis   (n = 47) 0–3.0 6. N. (N.) schmidti   (n = 5)

7. N. (N.) emyditoides   (n = 26)

8. N. (N.) panucensis   (n = 4)

9. N. (N.) chimalapasensis   (n = 2)

10. N. saginata   (n = 1)

continued.

TABLE 1. Specimens information: collection sites (CS), samples analyzed for DNA, species analyzed, specimens examined (N), host species, localities, GenBank accession number and catalog number (CNHE) for specimens studied in this work. Sequences marked with an asterisk (*) were obtained in the current study.

Samples (DNA) 1-3 4-8 9-12 Species N. (N.) brentnickoli   N. (N.) brentnickoli   N. (N.) brentnickoli   N Host 3 Dormitator latifrons   5 D. latifrons   4 D. latifrons   Locality Todos los Santos, Baja California Sur, México Oasis San José del Cabo, Baja California Sur, México Topolobampo,Sinaloa,México GenBank KR086167-KR086169* KR086170-KR086174* KR086175-KR086178* Specimensdeposited CNHE 8586
13-17 N. (N.) brentnickoli   5 D. latifrons   El Huizache, Sinaloa, México KR086179-KR086183* 8587
18-22 N. (N.) brentnickoli   5 D. latifrons   Vía Ferrea, Escuinapa, Sinaloa, México KR086184-KR086188* 8588
23-27 N. (N.) brentnickoli   5 D. latifrons   Nuevo Vallarta, Nayarit, México KR086189-KR086193*  
28-33 34 N. (N.) brentnickoli   N. (N.) brentnickoli   6 D. latifrons   1 D. latifrons   Estero Chamela, Jalisco, México Río Cuitzmala, Jalisco, México FJ388990, KR086194-KR086198* FJ388989 8589
35-39 N. (N.) brentnickoli   5 D. latifrons   Laguna de Cuyutlan, Colima, México KR086199-KR086203*  
40-49 50-54 N. (N.) brentnickoli   N. (N.) brentnickoli   10 D. latifrons   5 D. latifrons   Estero Boca de Apiza, Michoacán, México Estero Huahua, Michoacán, México JN830782-JN830786, KR086204-KR086208* KR086209-KR086213* 8176 8177
55-59 60-64 65-66 N. (N.) brentnickoli   N. (N.) brentnickoli   N. (N.) brentnickoli   5 D. latifrons   5 D. latifrons   2 D. latifrons   Estero Mexcalhuacan, Michoacán, México Estero Barra de Pichi, Michoacán, México Laguna de Coyuca, Guerrero, México JN830777- JN830781 KR086214-KR086218* JN830775, JN830776 8173 8174 8175
HWML

Howard W. Manter Laboratory of Parasitology

USNPC

United States National Parasite Collection

DNA

Department of Natural Resources, Environment, The Arts and Sport