Pythonides latemarginatus Grishin, 2023

Zhang, Jing, Cong, Qian & Grishin, Nick V., 2023, Supplementary Materials and Appendix, Insecta Mundi 2023 (26), pp. 1-115 : 18-19

Pythonides latemarginatus Grishin , new species

https://zoobank.org/ 7434EACB-635C-435F-A365-2932F2749590

( Fig. 2 part, 37–38, 250–251)

Definition and diagnosis. Specimens from Panama identified as Pythonides jovianus amaryllis Staudinger, 1876 (type locality in Panama and Colombia) are sister to all Pythonides jovianus (Stoll, 1782) and are genetically differentiated from it ( Fig. 2): e.g., their COI barcodes differ by 3.5% (24 bp) from P. j. amaryllis, and therefore they represent a new species. This new species keys to P. j. amaryllis (E.41.3(a)) in Evans (1953) and differs from it by a broader brown marginal area on the hindwing and correspondingly smaller blue patch, but more extensive forewing postdiscal blue spotting, in particular having a blue streak by the inner margin and two prominent blue streaks near forewing tornus beneath ( Fig. 37–38). This species is not cryptic and is diagnosed reliably by phenotype. In DNA, a combination of the following base pairs is diagnostic in the nuclear genome: aly 2178.10.1:C78T, aly 2178.10.1:C96T, aly363.24.5:G81A, aly522.12.2:G39A, aly9588.7.2:A27G, and COI barcode: T49A, T268C, T385C, T457C, T499C.

Barcode sequence of the holotype. Sample NVG-18018B04, GenBank OR837638, 658 base pairs: AACTTTATATTTTATTTTTGGAATTTGAGCTGGAATAGTAGGAACATCATTAAGTTTATTAATTCGAACTGAATTAGGTAATCCCGGTTCATTAATT GGAGATGATCAAATTTATAATACTATTGTAACTGCTCATGCTTTTATTATAATTTTCTTTATAGTAATACCAATTATAATTGGAGGATTTGGAAATT GACTAGTACCCCTTATACTAGGAGCTCCTGATATAGCATTCCCTCGAATAAATAATATAAGTTTTTGATTACTCCCCCCTTCTCTTACATTATTAAT TTCAAGAAGTATTGTTGAAAATGGAACTGGAACAGGATGAACAGTATACCCCCCTCTTTCCTCTAATATTGCTCATCAAGGATCTTCTGTTGACTTA

GCTATTTTTTCCTTACACCTAGCTGGTATTTCATCAATTTTAGGAGCTATTAATTTTATTACTACAATCATTAATATACGAATTAGAAATTTATCAT TTGATCAAATACCCTTATTTGTTTGAGCTGTAGGAATTACAGCATTATTATTATTATTATCATTACCTGTTTTAGCCGGAGCTATTACCATATTATT AACTGACCGAAATTTAAATACATCATTTTTTGATCCAGCTGGTGGAGGAGATCCAATTTTATATCAACATTTATTT

Type material. Holotype: ♂ deposited in the National Museum of Natural History , Smithsonian Institution,

Washington, DC, USA ( USNM), illustrated in Fig. 37–38, bears the following four rectangular labels, three white: [ PANAMA: Panama | Cerro Campana 2500′ | 8°41′N 79°55′W | August 1970 | leg. G.B.Small], [DNA sample ID: | NVG-18018B04 | c/o Nick V. Grishin], [USNMENT | {QR Code} | 01450934], and one red [HOLOTYPE ♂ | Pythonides | latemarginatus Grishin ]. Paratype: 1♂ NVG-18018B03 USNMENT_01450933 Panama: Darien, Cana, 750 m, 15-Jun-1983, G. B. Small leg. [ USNM].

Type locality. Panama: Panama Province, Cerro Campana, elevation 2500 ft, GPS 8.6833, −79.9167.

Etymology. The name is Latin for the broad (dark) margin and is a masculine adjective. Distribution. Panama.