Lychnuchus (Enosis) valle Grishin, 2023

Zhang, Jing, Cong, Qian & Grishin, Nick V., 2023, Supplementary Materials and Appendix, Insecta Mundi 2023 (26), pp. 1-115 : 68

Lychnuchus (Enosis) valle Grishin , new species

https://zoobank.org/ 97BEB2D4-9C1E-42D1-82F5-A1E7102CFF7F

( Fig. 7 part, 167–168, 396–398)

Definition and diagnosis. Phylogenetic trees reveal that two females from eastern Colombia identified as Lychnuchus (Enosis) topo (Nicolay, 1980) (type locality in Ecuador) show prominent genetic differentiation from it ( Fig. 7): e.g., their COI barcodes differ by 5.8% (38 bp), and therefore represent a new species. This new species differs from L. topo , which is known only from males, by vestigial or lacking pale dot on the ventral hindwing and darker tornal area of the ventral forewing. Due to the cryptic nature of this species, most reliable identification is achieved by DNA and a combination of the following base pairs is diagnostic in the nuclear genome: aly320.2.13:T495C, aly1603.33.3:G145A, aly770.42.5:A69T, aly 2096.2.3:G151C, aly3370.5.2:C48A, and COI barcode: T10C, T64C, T451C, A535T, T574C.

Barcode sequence of the holotype. Sample NVG-19021G09, GenBank OR837698, 658 base pairs: AACTTTATACTTTATTTTTGGAATTTGAGCAGGAATATTAGGAACTTCTTTAAGATTATTAATCCGTACAGAACTAGGTAATCCAGGTTCTTTAATT GGAGATGATCAAATTTATAATACTATTGTTACAGCCCATGCTTTTATTATAATTTTCTTTATAGTTATACCTATTATAATTGGAGGATTTGGTAATT GATTAGTTCCTTTAATATTAGGAGCACCTGATATAGCTTTCCCACGAATAAATAACATAAGATTTTGAATATTACCTCCCTCTTTAATGTTATTGAC TTCAAGAAGTATCGTAGAAAATGGTGCAGGTACAGGATGAACAGTTTATCCTCCTTTATCTTCTAATATTGCTCATCAAGGTTCTTCTGTTGACTTA GCTATTTTTTCCTTACATTTAGCAGGAATTTCATCTATTTTAGGAGCTATTAATTTTATTACCACAATTATTAATATACGAATTAAAAATATATCAT TTGATCAAATACCTTTATTTGTTTGATCAGTAGGTATTACAGCCTTACTTTTACTTTTATCTTTACCTGTTTTAGCTGGAGCTATTACCATACTTCT AACTGATCGAAATTTAAATACTTCATTTTTTGATCCAGCCGGAGGAGGAGACCCTATTTTATACCAACATTTATTT

Type material. Holotype: ♀ deposited in the National Museum of Natural History , Smithsonian Institution, Washington, DC, USA ( USNM), illustrated in Fig. 167–168, bears the following five rectangular labels, four white: [Jan.29, 1989 | Carret del Mar | to Dapa Rd., 1800 m | Valle, Colombia | J. B. Sullivan], [Genit. Vial | SRS-3248], [DNA sample ID: | NVG-19021G09 | c/o Nick V. Grishin], [USNMENT | {QR Code} | 01532704], and one red [HOLOTYPE ♀ | Lychnuchus (Enosis) | valle Grishin ] . Paratype: 1♀ NVG-22035H06 Colombia: Valle, San Antonio, above Cali, 1900 m, 2-Feb-1987, J. B. Sullivan leg., genitalia SRS-3224 [ USNM].

Type locality. Colombia: Valle, Carret del Mar to Dapa Rd., elevation 1800 m.

Etymology. The name is for the type locality and is a noun in apposition.

Distribution. Eastern Colombia.