Agathymus galeana Grishin, 2023

Zhang, Jing, Cong, Qian & Grishin, Nick V., 2023, Supplementary Materials and Appendix, Insecta Mundi 2023 (26), pp. 1-115 : 88

Agathymus galeana Grishin , new species

https://zoobank.org/ 1705C581-B49C-4679-9A6C-B1ACDD11F9CB

( Fig. 8 part, 219–220)

Definition and diagnosis. Phylogenetic trees reveal that a specimen of Agathymus H. Freeman, 1959 (type species Megathymus neumoegeni W. H. Edwards, 1882 ) from Nuevo Leon Mexico is not closely related to any of the known species of the genus and is a distant sister to both Agathymus estelleae (D. Stallings and Turner, 1958) (type locality in Mexico: Nuevo Leon) and Agathymus remingtoni (D. Stallings and J. Turner, 1958) (type locality in Mexico: Hidalgo) ( Fig. 8): COI barcode differences from them are 6.2% (41 bp), and 5.3% (35 bp), respectively, while being phenotypically dissimilar to them. This new species is more similar to Agathymus hoffmanni (H. Freeman, 1952) (type locality in Mexico: “Valle de Mexico ”), among others, in its orange coloration and pattern of spots but differs from it and other species by rounder wings, uniformly gray ventral hindwing without any spots and blotches, relatively narrow dorsal hindwing orange postdiscal band contrasting with broader orange spots on forewing overscaled with orange at its base, and orange along the cubitus in forewing discal cell. Due to the lack of additional specimens and unknown phenotypic variation, most reliable identification is achieved by DNA and a combination of the following base pairs is diagnostic in the nuclear genome: aly5773.3.3:C118T, aly5773.3.3:T428C, aly 2874.12.6:C66T, aly3850.2.2:T84A, aly 2041.8.3:T41G, aly2012.46.3:A132A (not G), aly5729.2.6:A854A (not C), aly536.163.5:T18T (not G), aly798.8.13:G48G (not C), aly16.30.9:A241A (not G), and COI barcode: A37G, T59A, T136C, T346C, 581C.

Barcode sequence of the holotype. Sample NVG-1895, GenBank OR837723, 658 base pairs: TACTTTATATTTTATTTTTGGTATTTGAGCAGGTATGTTAGGAACATCTTTAAGATTAATAATTCGTACTGAATTAGGAAATCCAGGATCATTAATT GGAGATGATCAAATTTATAATACCATTGTAACAGCTCACGCTTTTATTATAATTTTTTTCATAGTTATACCTATTATAATTGGAGGATTTGGTAATT GATTAGTACCTTTAATATTAGGAGCACCTGATATAGCTTTTCCCCGTATAAATAACATAAGATTTTGAATATTACCCCCCTCATTAATATTACTAAT CTCAAGAAGAATTGTAGAAAATGGTGCAGGAACAGGATGAACTGTATACCCTCCCCTATCTTCTAATATTGCACATCAAGGTTCTTCAGTTGATTTA GCAATTTTTTCCCTTCATTTAGCAGGTATCTCTTCTATTTTAGGAGCTATTAATTTTATTACTACAATTATTAATATACGAATTAAAAATTTATCAT TTGATCAAATACCTCTATTTGTATGATCTGTTGGAATTACTGCTTTATTATTATTATTATCTCTACCTGTTTTAGCTGGAGCTATTACTATACTTCT AACAGATCGAAATTTAAATACTTCTTTTTTTGACCCTGCTGGAGGAGGAGATCCAATTTTATATCAACACTTATTT

Type material. Holotype: ♀ deposited in the Texas A&M University Insect Collection, College Station , TX, USA

( TAMU), illustrated in Fig. 219–220, bears the following six rectangular labels, five white: [ MEXICO: | NUEVO LEON | ± 40 Km. WSW | Linares], [♂], [coll. | 21-IX-1977 | Roy O. Kendall | & C. A. Kendall], [DNA sample ID: |

NVG-1895 | c/o Nick V. Grishin], [ NVG 140104-44], and one red [HOLOTYPE ♀ | Agathymus | galeana Grishin ]. Type locality. Mexico: Nuevo Leon, ca 40 km WSW of Linares.

Etymology. The name is given for the type locality of this species near Galeana in Mexico and is a noun in apposition.

Distribution. Currently known only from the holotype collected in Nuevo Leon, Mexico.