taxonID	type	description	language	source
03D26F2DFFF2D604A133F992A9D95EDE.taxon	description	A laboratory isogenic strain of G. turnbulli that had been obtained from a local pet store and maintained in a stock fish population in our laboratory since 2013 (Tadiri et al., 2013, 2016) was used as the source of infection. We used one infected female fish from the parasite-infected stock population as the donor to infect all recipient fish. Donor and recipients were anaesthetized in a solution of 0.02 % Tricaine methanesulfonate (MS- 222). We used a standard infection procedure (Scott, 1982). In brief, under a dissecting microscope, we used tweezers to transport a scale with an attached parasite from the donor fish to the caudal fin of the recipient. Subsequently, we visually confirmed that the parasite was attached to its new host. The whole procedure lasted less than 2 min per fish and was performed only once. Infection dynamics were recorded every second day on anaesthetized individuals using a dissecting microscope to count the number of Gyrodactylus parasites. This procedure lasted less than 2 min per fish. All control fish were anaesthetized for sham infection every 2 days, so that they went through the same procedures as the infected fish. Approval for animal care and research was obtained from the McGill University Animal Care Committee (AUP 2014 - 7547) in compliance with the Canadian Council on Animal Care.	en	Blondel, L., Klemet-N'Guessan, S., Hendry, A. P., Scott, Marilyn E. (2024): Parasite load, rather than parasite presence, decreases upstream movement in Trinidadian guppies Poecilia reticulata. Journal of Fish Biology 105 (1): 177-185, DOI: 10.1111/jfb.15771, URL: https://doi.org/10.1111/jfb.15771
03D26F2DFFF2D607A133FD7FAF5D5AE4.taxon	description	We used second-generation laboratory-born male and female P. reticulata from parents that had been originally collected from a low-predation environment, with only one species of predator, Rivulus hartii located in the upper Turure River (GPS coordinates: 10.6903, 61.1638) and transported in March 2015 to our laboratory in Montréal, Canada. One week before the start of the experiment, individuals were haphazardly selected and assigned to two tanks of 10 fish each for acclimation. These tanks were kept in a room with natural light and contained artificial gravel at the bottom and a piece of broken flowerpot for shelter. Temperature was not measured in these tanks. The fish were fed daily with ad libitum brine shrimp throughout the experiment. One fish in each group died before the experiment, leaving us with nine fish per group (six females and three males). Each fish was weighed after the first and the second rheotaxis trials. Just after the first rheotaxis trial and for the duration of the experiment, each individual was isolated and maintained in a 1.8 - L tank within a flow-through system (Aquaneering Inc., San Diego, CA, USA). Immediately after the end of the experiment, fish were returned to a “ retirement stock tank ” with conspecifics.	en	Blondel, L., Klemet-N'Guessan, S., Hendry, A. P., Scott, Marilyn E. (2024): Parasite load, rather than parasite presence, decreases upstream movement in Trinidadian guppies Poecilia reticulata. Journal of Fish Biology 105 (1): 177-185, DOI: 10.1111/jfb.15771, URL: https://doi.org/10.1111/jfb.15771
