identifier	taxonID	type	CVterm	format	language	title	description	additionalInformationURL	UsageTerms	rights	Owner	contributor	creator	bibliographicCitation
03FF0200FF9FFFF71FFAAD17FA85F822.text	03FF0200FF9FFFF71FFAAD17FA85F822.taxon	http://purl.org/dc/dcmitype/Text	http://rs.tdwg.org/ontology/voc/SPMInfoItems#GeneralDescription	text/html	en	Dna	<div><p>DNA Extraction, PCR Amplification, and Sequencing</p><p>Fungal genomic DNA was extracted from 20-day-old mycelia using the Trelief™ Plant Genomic DNA Kit (Beijing Qingke Biotech), following the manufacturer’s instructions.Polymerase chain reaction (PCR) was performed to amplify the internal transcribed spacer (ITS) region, the large subunit ribosomal RNA (LSU), the second largest subunit of RNA polymerase II (rpb 2), and beta-tubulin (tub 2) genes using the primer pairs ITS9mun/ITS4_KYo1 (Toju et al. 2012), LR0R (Hopple &amp; Vilgalys 1999)/ LR5 (Cubeta et al. 1991), fRPB2-7cR/fRPB2-5F (Voglmayr et al. 2016), and TI/T22 (O'Donnel &amp; Cigelnik 1997), respectively. The PCR reaction was set up at 30 μL volume, containing 15 μL of PCR Master Mix (CoWin Biosciences, Taizhou, China), 11 μL of ddH 2 O, 1 μL each of forward and reverse primers (10 μM), and 2 μL of DNA template. The PCR conditions were performed as follows: 94 °C for 5 min, followed by 35 cycles of denaturation at 94 °C for 1 min, annealing at 55 °C for a suitable time, extension at 72 °C for 2 min, and a final extension of 72 °C for 10 min. The annealing time of each gene was 50 s for ITS, LSU and tub 2; 2 min for rpb 2. The PCR products were analyzed by 1% agarose gel electrophoresis, and Sanger sequencing was carried out by Sangon Biotech Co., Ltd. Raw sequence data were processed using SeqMan v.7.1.0 software, which was used to assess chromatogram quality, trim low-quality bases at terminals, and assemble bidirectional sequencing reads.</p></div>	https://treatment.plazi.org/id/03FF0200FF9FFFF71FFAAD17FA85F822	Public Domain	No known copyright restrictions apply. See Agosti, D., Egloff, W., 2009. Taxonomic information exchange and copyright: the Plazi approach. BMC Research Notes 2009, 2:53 for further explanation.		Plazi	Ding, Peng-Cheng;Madhushan, Asanka;Samarakoon, Milan C.;Liu, Jian-Kui;Maharachchikumbura, Sajeewa S. N.	Ding, Peng-Cheng, Madhushan, Asanka, Samarakoon, Milan C., Liu, Jian-Kui, Maharachchikumbura, Sajeewa S. N. (2025): A new species of Hypoxylon (Hypoxylaceae, Xylariales) from Sichuan Province, China. Phytotaxa 715 (3): 229-244, DOI: 10.11646/phytotaxa.715.3.3, URL: https://doi.org/10.11646/phytotaxa.715.3.3
03FF0200FF97FFFC1FFAA89BFCD6F9B0.text	03FF0200FF97FFFC1FFAA89BFCD6F9B0.taxon	http://purl.org/dc/dcmitype/Text	http://rs.tdwg.org/ontology/voc/SPMInfoItems#GeneralDescription	text/html	en	Hypoxylon omphalostiolatum P. C. Ding, Madhushan & Maharachch. 2025	<div><p>Hypoxylon omphalostiolatum P. C. Ding, Madhushan &amp; Maharachch., sp. nov. (FIGURE 2)</p><p>MycoBank: 859931</p><p>Etymology: The name reflects the umbilicate (omphalos) ostioles (ostiolatum).</p><p>Saprobic on the decaying branches of an unidentified plant. Sexual morph: Stromata superficial, pulvinate to effused-pulvinate, and gregarious, with inconspicuous perithecial mounds, surface violet-brown, multi-layered perithecial mounds, spreading over a large area of the host surface, yellow pigments in 10% KOH. Perithecia (= 229 × 204 μm, n = 10) globose to ellipsoid, with an ostiole. Ostioles inconspicuous, umbilicate, usually overlain with conspicuous white substance. Peridium 22–69 μm (= 46.3 μm, n = 20) wide, multi-layered, outer layer dark purple with thick-walled cells of textura angularis, inner layer hyaline with thin-walled cells of angularis and prismatica. Paraphyses 2–9 μm (= 6.68 μm, n = 30) wide, septate, unbranched, hyaline, cellular, minutely guttulate, and embedded in a gelatinous matrix. Asci 84–132 × 6–12 μm (= 106.23 × 8.51 μm, n = 30), ascospore bearing part 56–85 μm (= 70.5 μm, n = 30), non-bearing part 33–48 μm (= 39.4 μm, n = 30), 8-spored, unitunicate, cylindrical or clavate, and long-pedicellate, J- apical ring in Melzers’reagent. Ascospores 7–12 × 3–7 μm (= 9.45 × 5.2 μm, n = 50), uniseriate, ellipsoidal, narrow at both ends, smooth-surfaced, aseptate, initially hyaline to yellowish brown, dark brown at maturity, and contain 1 or 2 large guttules to multiple small guttules, perispore dehiscent in 10% KOH. Asexual morph: Not observed.</p><p>Colony characteristics: Conidia germinate on PDA within 24 h at 24 °C. Colonies on PDA reaching 42 mm in diameter after 3 weeks at 25 °C. Mycelia superficial, surface slightly rough, with undulate margin, forming small, from above, white; reverse, blackish brown.</p><p>Materials examined: CHINA, Sichuan Province, Liangshan Yi Autonomous Prefecture, Puge County, Luoji Mountain Town, 27˚42'44'' N, 102˚24'07'' E, 2140 m, unknown host, 20 June 2024, P. C. Ding XCJ4-6-1(HKAS 149990, holotype), ex-type CGMCC 3.28999 = UESTCC 25.0246 ; ibid., XCJ4-6- 2 paratype (UESTCC 25.0247) .</p><p>Notes: In the multi-gene phylogeny, our isolates of H. omphalostiolatum form a sister clade with H. guiyangense (KUNCC 23.15543, KUNCC 23.15546), with (87 ML/ 0.96 BYPP) bootstrap support (FIGURE 1). The BLASTn analysis of H. omphalostiolatum type strain (CGMCC 3.28999) and H. guiyangense (KUNCC 23.15543; type strain) shows 99% identity (508/515 bp, 0 gaps) in ITS, 99% identity (730/734 bp, 3 gaps) in LSU, and 96% identity (942/977 bp, 3 gaps) in tub 2. Also, H. omphalostiolatum (CGMCC 3.28999) and other closely related H. guizhouense (KUNCC 23-15544) shows 98% identity (506/516 bp, 1 gap) in ITS, 99% identity (728/734 bp, 1 gap) in LSU, and 95% identity (941/986 bp, 17 gaps) in tub 2.</p><p>Morphologically, the stromata surface of H. omphalostiolatum (CGMCC 3.28999) is dark brick to dark brown, while the stromata of H. guizhouens e and H. guiyangense are violet-brown and yellowish-brown, respectively. Moreover, the former covers a larger area of the host substrate than the latter two. Perithecia of H. omphalostiolatum are larger (229 × 204 μm) than those of H. guiyangense (135 × 143 μm) and H. guizhouense (195 × 203 μm). Meantime, H. omphalostiolatum has inconspicuous, umbilicate ostioles. The asci of H. omphalostiolatum are also longer (84–132 μm) than H. guiyangense (80–100 μm) and H. guizhouense (80–100 μm). Hypoxylon omphalostiolatum resembles type descriptions of H. guiyangense and H. guizhouense, with hyaline to brown, ellipsoidal ascospores (Dissanayake et al. 2024). However, H. omphalostiolatum ascospores are wider (9.45 × 5.2 μm) compared to H. guiyangense (9.4 × 4.8 μm), but shorter than H. guizhouense (10 × 4.8 μm). In addition, both H. omphalostiolatum and H. guizhouense possess two guttules, whereas H. guiyangense contains more, and the ostiole of H. omphalostiolatum is inconspicuous. H. guiyangense and H. guizhouense were identified on dead twigs of an unidentified host in Guiyang and Guizhou city (Dissanayake et al. 2024). Based on combined phylogenetic analysis and detailed morphological evidence, we conclude our collection is a new species, H. omphalostiolatum .</p></div>	https://treatment.plazi.org/id/03FF0200FF97FFFC1FFAA89BFCD6F9B0	Public Domain	No known copyright restrictions apply. See Agosti, D., Egloff, W., 2009. Taxonomic information exchange and copyright: the Plazi approach. BMC Research Notes 2009, 2:53 for further explanation.		Plazi	Ding, Peng-Cheng;Madhushan, Asanka;Samarakoon, Milan C.;Liu, Jian-Kui;Maharachchikumbura, Sajeewa S. N.	Ding, Peng-Cheng, Madhushan, Asanka, Samarakoon, Milan C., Liu, Jian-Kui, Maharachchikumbura, Sajeewa S. N. (2025): A new species of Hypoxylon (Hypoxylaceae, Xylariales) from Sichuan Province, China. Phytotaxa 715 (3): 229-244, DOI: 10.11646/phytotaxa.715.3.3, URL: https://doi.org/10.11646/phytotaxa.715.3.3
