identifier	taxonID	type	CVterm	format	language	title	description	additionalInformationURL	UsageTerms	rights	Owner	contributor	creator	bibliographicCitation
0398C033FFE53317FF61FC4C5B4AF910.text	0398C033FFE53317FF61FC4C5B4AF910.taxon	http://purl.org/dc/dcmitype/Text	http://rs.tdwg.org/ontology/voc/SPMInfoItems#GeneralDescription	text/html	en	Dna extraction PCR	<div><p>DNA extraction, PCR amplification, and sequencing</p><p>Genomic DNA was extracted from fresh fungal mycelia that had been cultured on PDA for 2–4 weeks using the Biospin Fungus Genomic DNA Extraction Kit-BSC14S1 (BioFlux, China), following the manufacturer's instructions. The extracted DNA was stored at -20 °C for subsequent use. Polymerase chain reaction (PCR) was conducted targeting four loci: SSU, ITS, LSU, and tef 1-α genes, with primer pairs LR5/LR0R (Vilgalys &amp; Hester, 1990), ITS5/ITS4, NS1/ NS4 (White et al. 1990), and EF1-983F/EF1-2218R (Rehner &amp; Buckley 2005). The PCR reactions were prepared in a total volume of 25 µL, consisting of 12.5 µL of 2× Bench Top™ Taq Master Mix, 8.5 µL of ddH 2 O, 2 µL of each forward and reverse primer, and 2 µL of the DNA template. The same PCR conditions for the LSU, ITS, SSU, and tef 1-α genes involved an initial denaturation at 94 °C for 3 minutes, followed by 35 cycles of 94 °C for 45 seconds, 55 °C for 50 seconds, and 72 °C for 60 seconds, with a final extension at 72 °C for 10 minutes. The PCR products were purified and sequenced by Sangon Biotechnology Co., Ltd. (Shanghai, China). All new sequences generated in this study were submitted to GenBank (http://www.ncbi.nlm.nih.gov/genbank/) and are detailed in Table 1.</p></div>	https://treatment.plazi.org/id/0398C033FFE53317FF61FC4C5B4AF910	Public Domain	No known copyright restrictions apply. See Agosti, D., Egloff, W., 2009. Taxonomic information exchange and copyright: the Plazi approach. BMC Research Notes 2009, 2:53 for further explanation.		Plazi	Han, Meiyan;Karunarathna, Samantha C.;Lu, Li;Zheng, Dege;Dai, Dongqin;Suwannarach, Nakarin;Kumla, Jaturong;Elgorban, Abdallah M.;Zhang, Lijuan;Chukeatirote, Ekachai;Tibpromma, Saowaluck	Han, Meiyan, Karunarathna, Samantha C., Lu, Li, Zheng, Dege, Dai, Dongqin, Suwannarach, Nakarin, Kumla, Jaturong, Elgorban, Abdallah M., Zhang, Lijuan, Chukeatirote, Ekachai, Tibpromma, Saowaluck (2025): Pseudodictyosporium yunnanensis sp. nov. (Dictyosporiaceae, Pleosporales) from dead twigs of Coffea arabica in China. Phytotaxa 711 (1): 28-42, DOI: 10.11646/phytotaxa.711.1.2, URL: https://doi.org/10.11646/phytotaxa.711.1.2
0398C033FFE23312FF61F8D85C3EF88A.text	0398C033FFE23312FF61F8D85C3EF88A.taxon	http://purl.org/dc/dcmitype/Text	http://rs.tdwg.org/ontology/voc/SPMInfoItems#GeneralDescription	text/html	en	Pseudodictyosporium yunnanensis M. Y. Han & Tibpromma 2025	<div><p>Pseudodictyosporium yunnanensis M.Y. Han &amp; Tibpromma, sp. nov. Fig. 2</p><p>Index Fungorum number: IF903903, Facesoffungi number: FoF 17940</p><p>Etymology:— The name reflects the type location, “ Yunnan ” Province, China. Holotype: GMB-W1496</p><p>Saprobic on dead twigs of Coffea arabica . Sexual morph: Undetermined. Asexual morph: Hyphomycetous. Conidiomata on natural substratum sporodochia, superficial, compact, punctiform to effuse, scattered or in small groups, with base attached on surface of host plant, dark brown to black. Mycelium immersed, composed of septate, branched, subhyaline to pale brown, smooth-walled hyphae. Conidiophores 21–55 × 4.7–9.4 μm (x̄ = 34.4 × 6.8 μm, n = 30), micronematous, septate, often constricted at septa, simple, hyaline to pale brown, cylindrical or swollen to 4–10.4 μm wide, somewhat moniliform, verrucose or smooth. Conidiogenous cells 6.8–10.4 × 6.9–7.2 μm (x̄ = 8.5 × 6.5 μm, n = 30), integrated, holoblastic, polyblastic, doliiform, cylindrical or inflated, terminal or intercalary, determinate. Conidia 20.2–27.3 × 13.1–19.8 μm (x̄ = 23.5 × 15.2 μm, n = 30), dictyosporous, acrogenous and pleurogenic, solitary, dry, most cheiroid, some oblong with obtuse ends or oval, pale brown or brown, smooth-walled, multi-septate, consisting of a truncate basal cell on which three rows of cells arise parallelly and compactly with (2-) 3 rows in different planes, appendages absent.</p><p>Culture characteristics:— Conidia germinated on PDA medium within 24 h. Germ tubes produced from ends. Colonies on PDA reaching 15 mm diam. after one week at 25 °C in natural light. Mycelium superficial, with regular margins, white from above, orange-yellow in the center, and brown in the middle zone from below, paler toward the margins.</p><p>Material examined:— China, Yunnan Province, Pu'er City, Simao District (101°2'44'' E, 22°31'18'' N, 856.89 m), a dead twig of Coffea arabica, 1 August 2024, Meiyan Han, BG90 (GMB-W1496, holotype), ex-type GMBCC 2237, other living culture GMBCC 2238.</p><p>GenBank numbers:— ITS: PV618259, LSU: PV618339, SSU: PV620840, tef 1-α: PV631995 (GMBCC 2237, ex-type); ITS: PV618260, LSU: PV618340, SSU: PV620841, tef 1-α: PV631996 (GMBCC 2238).</p><p>Notes:— In the phylogenetic analyses, Pseudodictyosporium yunnanensis formed a distinct lineage within Pseudodictyosporium with statistical support (100 ML/1.00 BYPP, Fig. 1). In the NCBI blast results of sequences, ITS was 96.24% similar to P. elegans (NR_137148), LSU was 99.52% similar to P. wauense (MH875472), and tef 1- α was similar to P. thailandica (KX259526, 97.16%), while SSU had a high overlap of 96.37% with Dictyosporium tetrasporum (AB797229). Morphologically, our strain P. yunnanensis exhibits the typical characteristics of the genus, including conidia that consist of a truncate basal cell from which three rows of cells arise parallelly and compactly, with all three rows in different planes and the majority of the conidia are cheiroid in shape (Boonmee et al. 2016, Li et al. 2017). However, it can be distinguished from other species by differences in conidial size and color, as well as the size and morphology of conidiophores and conidiogenous cells (Kirschner et al. 2013, Boonmee et al. 2016, Hyde et al. 2016, Li et al. 2017). Furthermore, our new species exhibits distinctly swollen conidiophores and conidiogenous cells, with some nodes being transparent and others having wart-like protrusions or containing oil droplets within the enlarged portions (Fig. 2). And our new species shows multiple sites on the enlarged portions of the conidiogenous cells serve as points of conidial production, resulting in the formation of conidial chains or clusters (Fig. 2, Table 2). Therefore, based on morphological characteristics and multi-gene phylogenetic analysis, Pseudodictyosporium yunnanensis is introduced as a new species.</p></div>	https://treatment.plazi.org/id/0398C033FFE23312FF61F8D85C3EF88A	Public Domain	No known copyright restrictions apply. See Agosti, D., Egloff, W., 2009. Taxonomic information exchange and copyright: the Plazi approach. BMC Research Notes 2009, 2:53 for further explanation.		Plazi	Han, Meiyan;Karunarathna, Samantha C.;Lu, Li;Zheng, Dege;Dai, Dongqin;Suwannarach, Nakarin;Kumla, Jaturong;Elgorban, Abdallah M.;Zhang, Lijuan;Chukeatirote, Ekachai;Tibpromma, Saowaluck	Han, Meiyan, Karunarathna, Samantha C., Lu, Li, Zheng, Dege, Dai, Dongqin, Suwannarach, Nakarin, Kumla, Jaturong, Elgorban, Abdallah M., Zhang, Lijuan, Chukeatirote, Ekachai, Tibpromma, Saowaluck (2025): Pseudodictyosporium yunnanensis sp. nov. (Dictyosporiaceae, Pleosporales) from dead twigs of Coffea arabica in China. Phytotaxa 711 (1): 28-42, DOI: 10.11646/phytotaxa.711.1.2, URL: https://doi.org/10.11646/phytotaxa.711.1.2
