taxonID	type	description	language	source
03B087DAFFB8B7481EC747B12EC0FE4F.taxon	description	Figs 1 - 4	en	Pešić, Vladimir, Smit, Harry, Girão, Dinis, Iannucci, Alessio, Stur, Elisabeth, Ferreira, Sónia (2024): New records of aturid water mites from Portugal, with the integrative description of one new species (Acari, Hydrachnidia, Aturidae). Ecologica Montenegrina 80: 209-219, DOI: 10.37828/em.2024.80.19, URL: https://doi.org/10.37828/em.2024.80.19
03B087DAFFB8B7481EC747B12EC0FE4F.taxon	materials_examined	Material examined — Holotype ♂, dissected and slide mounted (RMNH), Portugal, Beja, Mértola, São João dos Caldeireiros, 37.626 ° N, 7.810 ° W, 17 May 2023, leg. Ferreira, Benitez-Bosco, Ekrem, Stur & Turaccio, sequenced (Voucher ID: BGE _ 00110 _ B 01; BOLD ID: BSNTN 488 - 23). Paratype: 1 ♂ (RMNH) same data as the holotype, preserved in Koenike-fluid (sequenced; BOLD ID: BSNTN 947 - 23).	en	Pešić, Vladimir, Smit, Harry, Girão, Dinis, Iannucci, Alessio, Stur, Elisabeth, Ferreira, Sónia (2024): New records of aturid water mites from Portugal, with the integrative description of one new species (Acari, Hydrachnidia, Aturidae). Ecologica Montenegrina 80: 209-219, DOI: 10.37828/em.2024.80.19, URL: https://doi.org/10.37828/em.2024.80.19
03B087DAFFB8B7481EC747B12EC0FE4F.taxon	diagnosis	Diagnosis (Female unknown) — Dorsal shield nearly as wide as long, posterior margin medially projecting; a pair of posteriorly directed, pointed sheaths located laterally near the posterior end of ventral shield; gonopore anteriorly surrounded by well sclerotized lips, Ac- 1 located on the surface of these sclerotized lips, numerous setae anteriorly in transverse line, and posteriorly from the level of Ac- 3; IV-L- 4 distally with one curved, spatula-shaped seta, IV-L- 4 digitiform ventral extension apically with three different setae (one longer flanked by two stout setae, one thicker, peg-like, the other thinner).	en	Pešić, Vladimir, Smit, Harry, Girão, Dinis, Iannucci, Alessio, Stur, Elisabeth, Ferreira, Sónia (2024): New records of aturid water mites from Portugal, with the integrative description of one new species (Acari, Hydrachnidia, Aturidae). Ecologica Montenegrina 80: 209-219, DOI: 10.37828/em.2024.80.19, URL: https://doi.org/10.37828/em.2024.80.19
03B087DAFFB8B7481EC747B12EC0FE4F.taxon	description	Description — Male (holotype) — Idiosoma dorsally 470 long and 493 wide, ventrally 500 long. Colour yellowish with one unpaired anterior and a pair of posterolateral violet marks (see Fig. 1 A). Dorsal shield nearly as wide as long, posterior margin medially projecting and with excretory pore placed terminally dorsal on a projection (Fig. 1 A). Anterior margins of Cx-I extending slightly beyond frontal margin, gnathosomal bay 128 deep; distance between IV-L insertion 271. A pair of posteriorly directed pointed sheaths located laterally near the posterior end of ventral shield (Figs. 1 A, 2). Posterior margin of the venter forming a rounded concave ridge. Gonopore in distal part flanked by welldeveloped sclerotized lips, Ac- 1 located on the surface of these sclerotized lips, acetabula arranged in an obtuse triangle, numerous fine setae, anteriorly from the level of Ac- 2 longer, and posteriorly from the level of Ac- 3 shorter (Fig. 2). Ejaculatory complex 116 long. Palp as illustrated in Figs. 2 B-C: length / height: P- 1, 49 / 19; P- 2, 73 / 52; P- 3, 36 / 33; P- 4, 113 / 36; P- 5, 36 / 20 /; palpal segment relatively stout (length / height ratio P- 2, 1.4; P- 3, 1.1; P- 4, 3.1); P- 1 with one dorsal seta; P- 2 ventral margin nearly straight, distally forming a short blunt projection; P- 3 ventral margin concave; P- 4 proximally narrow, in the centre, near the insertion of two ventral setae slightly elevated to form an obtuse projection near the insertion of the stronger ventral seta, a slightly thickened seta and a finer seta laterally on a further blunt elevation, dorsal surface (medially and ventrally) of P- 4 with several fine setae. Capitulum ventral length 102; chelicera 134 long. Legs: I-L and II-L as shown in Figs. 4 A-B, surface of I-L- 6 and II-L- 6 with numerous fine setae; III-L as illustrated in Fig. 4 C, III-L- 5 distally enlarged, here with four dorsal club-shaped setae, one of them longer than the other three (Fig. 4 C inset); IV-L- 4 - 6 as shown in Figs. 4 D-E; IV-L- 4 distally enlarged, here bearing six to seven setae, two of them heavy (one curved, spatula-shaped seta, shifted more to ventrodistal margin, the other straight and longer), the remaining slender and pointed, and forming a prominent digitiform ventral extension apically with three different setae (one longer flanked by two stout setae, one thicker, peg-like, the other thinner); IV-L- 5 thickened, length / height ratio 3.3, dorsal margin curved, ventral margin, with scattered setae, in proximal part a peg-like setae; IV-L- 6 slightly curved, length / height ratio 5.3, ventrally with a row of longer setae; claws modified as given in Figs. 3 A (inset), anterior claw longer than posterior claw. Number of swimming setae: II-L- 4. 2, II-L- 5: 3; III-L- 4: 5; IV-L- 5. 6. Dorsal length: I-L- 2 - 6: 55, 81, 122, 139, 131; II-L- 1 - 6: 72, 63, 91, 134, 144, 128; III-L- 1 - 6: 66, 69, 94, 103. 98, 134; IV-L- 1 - 6: 116, 88, 80, 69, 188, 166. Female: unknown.	en	Pešić, Vladimir, Smit, Harry, Girão, Dinis, Iannucci, Alessio, Stur, Elisabeth, Ferreira, Sónia (2024): New records of aturid water mites from Portugal, with the integrative description of one new species (Acari, Hydrachnidia, Aturidae). Ecologica Montenegrina 80: 209-219, DOI: 10.37828/em.2024.80.19, URL: https://doi.org/10.37828/em.2024.80.19
03B087DAFFB8B7481EC747B12EC0FE4F.taxon	etymology	Etymology — The name refers to the geographical provenance (from Latin lusitanicus, Lusitanian, Portuguese) from where the new species is described. Species delimitation using DNA barcodes — We used a COI dataset with 79 sequences of water mite specimens of the family Aturidae listed in Table 1 and Supplement material 1 for phylogenetic analyses. Hungarohydracarus subterraneus from Bosnia and Herzegovina was used to root the tree. The NJ tree is presented in Fig. 5. The COI tree sequences retrieved from two specimens from Portugal, which form a unique cluster (BOLD: AFN 8479), appeared as a sister group (albeit with a low support) to the sequence belonging to Parabrachypoda montii, a rhitrobiontic species widely distributed in Europe. The mean K 2 P genetic distance between COI sequences of Parabrachypoda lusitanica sp. nov. and P. montii was estimated at 26.3 %.	en	Pešić, Vladimir, Smit, Harry, Girão, Dinis, Iannucci, Alessio, Stur, Elisabeth, Ferreira, Sónia (2024): New records of aturid water mites from Portugal, with the integrative description of one new species (Acari, Hydrachnidia, Aturidae). Ecologica Montenegrina 80: 209-219, DOI: 10.37828/em.2024.80.19, URL: https://doi.org/10.37828/em.2024.80.19
03B087DAFFB8B7481EC747B12EC0FE4F.taxon	discussion	Remarks — In regard to the position of the genital field (located close to the posterior margin of the ventral shield) and similar shape of palp (P- 2 with a very short, rounded distoventral projection) and setation of IV-L, the new species from Portugal is placed in genus Parabrachypoda. Currently, the latter genus, includes five species known from the Western Palaearctic, i. e. P. modesta (Koenike, 1911), known from Central and eastern Europe, P. baderi Di Sabatino & Cicolani, 1991, known from central Mediterranean (Italy: Abruzzo, Sicily, Sardinia; France: Corsica; Sabatino & Cicolani, 1991, Gerecke et al. 2014) and Turkey (Esen et al. 2014), Parabrachypoda mutila (Walter, 1928), known from the West Mediterranean area (Algeria, Tyrrhenian islands - Gerecke et al. 2014), P. montii (Maglio, 1924) known from central and southern Europe and P. orientalis Pešić & Esen, 2013. The latter species was originally described from Turkey (Esen et al. 2014), and includes also populations under the (erroneous) name of P. mutila from Crimea (Tuzovskij 1978, including also the reared larval stage) and Turkey (Pešić et al. 2006). From all these species, the new species from Portugal can be separated by the unique shape of dorsal shield (nearly as wide as long, posterior margin medially projecting) and genital field (gonopore anteriorly surrounded by well sclerotized lips, Ac- 1 located on surface of these sclerotized lips, numerous setae around the gonopore, anteriorly in transverse line, and in a field postero-medially of Ac- 3. Moreover, the presence of a pair of posteriorly directed pointed sheaths located laterally near the posterior end of ventral shield is an unique feature and not present in other species of the genus. In regard to IV-L setation, males of Para b rachypoda mutila resemble the new species in the presence of three different apical setae on the ventral digitiform extension of IV-L- 4 (one fine, longer seta flanked by two stout setae, one of them thicker than the other). However, in the latter species IV- L- 5 is much slenderer, with subparallel dorsal and ventral margins, bearing numerous longer ventral setae. In P. lusitanica IV-L- 5 is thickened and with more curved dorsal margin, more similar to B. orientalis, ventral setae in proximal part peg-like. Moreover, the distal margin of IV-L- 4 of P. mutila lacks the characteristic spatula-like seta present in the new species. Distribution — Portugal; known only from the type locality (Fig. 6) in the Alentejo region (Southern Portugal).	en	Pešić, Vladimir, Smit, Harry, Girão, Dinis, Iannucci, Alessio, Stur, Elisabeth, Ferreira, Sónia (2024): New records of aturid water mites from Portugal, with the integrative description of one new species (Acari, Hydrachnidia, Aturidae). Ecologica Montenegrina 80: 209-219, DOI: 10.37828/em.2024.80.19, URL: https://doi.org/10.37828/em.2024.80.19
03B087DAFFB2B7481EC746862D03F907.taxon	materials_examined	Material examined — Portugal, Bragança, Mirandela, Torre de Dona Chama, 41.665 ° N, 7.146 ° W, 256 m asl., 13 Jul. 2023 leg. Ferreira & Padilha, 1 ♂ (sequenced), dissected and slide mounted (RMNH). Remarks — The Portuguese specimen molecularly analyzed in this study keys out as Hexaxonopsis romijni with the key of Gerecke et al. (2016). This specimen forms a unique BIN (BOLD: AFW 0017). In the phylogenetic tree, the BIN is positioned as a sister clade of H. serrata from Montenegro. Distribution — Western and central Europe (Pešić et al. 2010; Gerecke et al. 2016); Turkey (Esen et al. 2011). New for Portugal.	en	Pešić, Vladimir, Smit, Harry, Girão, Dinis, Iannucci, Alessio, Stur, Elisabeth, Ferreira, Sónia (2024): New records of aturid water mites from Portugal, with the integrative description of one new species (Acari, Hydrachnidia, Aturidae). Ecologica Montenegrina 80: 209-219, DOI: 10.37828/em.2024.80.19, URL: https://doi.org/10.37828/em.2024.80.19
03B087DAFFB2B74A1EC7447E2CEEF82C.taxon	materials_examined	Material examined — Portugal, Guarda: Seia, Rio Alva, Praia Fluvial de Sabugueiro, 40.401 ° N, 7.64 ° W, 1021 m a. s. l., 24 Aug. 2023, leg. Ferreira, Benitez-Bosco & Padilha, 2 ♂, 1 ♀ (BGE _ 00227 _ A 01, BGE _ 00108 _ H 06, BGE _ 00108 _ H 08); Manteigas, Poio do Leão, 40.399 ° N, 7.541 ° W, 734 m a. s. l., 22 Aug. 2023, leg. Ferreira, Benitez-Bosco & Padilha, 1 ♀ (BGE _ 00108 _ D 05); Manteigas, Zêzere, Ponte dos Frades, 40.403 ° N, 7.526 ° W, 672 m a. s. l., 22 Aug. 2023, leg. Ferreira, Benitez-Bosco, Padilha & Stur 1 ♂ (BGE _ 00108 _ D 07), dissected and slide mounted (RMNH); Manteigas, Mondego, Covão da Ponte, 40.443 ° N, 7.514 ° W, 999 m a. s. l., 24 Aug. 2023, leg. Ferreira, Benitez-Bosco & Padilha, 1 ♀ (BGE _ 00108 _ G 02).	en	Pešić, Vladimir, Smit, Harry, Girão, Dinis, Iannucci, Alessio, Stur, Elisabeth, Ferreira, Sónia (2024): New records of aturid water mites from Portugal, with the integrative description of one new species (Acari, Hydrachnidia, Aturidae). Ecologica Montenegrina 80: 209-219, DOI: 10.37828/em.2024.80.19, URL: https://doi.org/10.37828/em.2024.80.19
03B087DAFFB2B74A1EC7447E2CEEF82C.taxon	discussion	Remarks — The specimens from Portugal morphologically match the description of Woolastookia rotundifrons following Pešić et al. (2010). The sequenced specimens from Portugal form a unique BIN: BOLD: AFV 8119, with the nearest neighbouring BIN being BOLD: AEE 0289 (p - distance 17.39 %), which includes specimens from Montenegro morphologically as well assigned to W. rotundifrons, indicating the need for taxonomic revision of this species from a wider geographical area.	en	Pešić, Vladimir, Smit, Harry, Girão, Dinis, Iannucci, Alessio, Stur, Elisabeth, Ferreira, Sónia (2024): New records of aturid water mites from Portugal, with the integrative description of one new species (Acari, Hydrachnidia, Aturidae). Ecologica Montenegrina 80: 209-219, DOI: 10.37828/em.2024.80.19, URL: https://doi.org/10.37828/em.2024.80.19
03B087DAFFB2B74A1EC7447E2CEEF82C.taxon	distribution	Distribution — Central, southern and eastern Europe (Pešić et al. 2010; Gerecke et al. 2016). New for Portugal. Acknowledgements Biodiversity Genomics Europe (Grant no. 101059492) is funded by Horizon Europe under the Biodiversity, Circular Economy and Environment call (REA. B. 3); co-funded by the Swiss State Secretariat for Education, Research and Innovation (SERI) under contract number 22.00173; and by the UK Research and Innovation under the Department for Business, Energy and Industrial Strategy’s Horizon Europe Guarantee Scheme. SF was funded by the FCT through the program ‘ Stimulus of Scientific Employment, Individual Support — 3 rd Edition’ (https: // doi. org / 10.54499 / 2020.03526. CEECIND / CP 1601 / CP 1649 / CT 0007). The authors would like to acknowledge Estação Biológica de Mértola, Mértola Municipality, CISE, Seia Municipality and Faculty of Sciences – University of Porto for the logistic support during the fieldwork and Laura Benitez-Bosco, Torbjørn Ekrem, Thomas S. Ekrem, Piotr Gadawski, Roman Hodunko, José Conde, Tiago Correia, António Rodrigues, Adriana Padilha and Rui Andrade for the great company during fieldwork. We thank Tom Goldschmidt (Munich) and Andrzej Zawal (Szczecin) whose constructive comments greatly improved this work.	en	Pešić, Vladimir, Smit, Harry, Girão, Dinis, Iannucci, Alessio, Stur, Elisabeth, Ferreira, Sónia (2024): New records of aturid water mites from Portugal, with the integrative description of one new species (Acari, Hydrachnidia, Aturidae). Ecologica Montenegrina 80: 209-219, DOI: 10.37828/em.2024.80.19, URL: https://doi.org/10.37828/em.2024.80.19
