identifier	taxonID	type	CVterm	format	language	title	description	additionalInformationURL	UsageTerms	rights	Owner	contributor	creator	bibliographicCitation
91653536FFA7D15BFF21FCB7FD16FEAC.text	91653536FFA7D15BFF21FCB7FD16FEAC.taxon	http://purl.org/dc/dcmitype/Text	http://rs.tdwg.org/ontology/voc/SPMInfoItems#GeneralDescription	text/html	en	Sellaphora ashinovii Glushchenko, Tseplik, Maltsev, Genkal, Iurmanov & Kulikovskiy 2025	<div><p>Sellaphora ashinovii Glushchenko, Tseplik, Maltsev, Genkal, Iurmanov &amp; Kulikovskiy sp. nov. (Figs 1–25)</p><p>Holotype. Slide no. 05556 (represented here by Figure 13), deposited in the Herbarium of K.A. Timiryazev Institute of Plant Physiology, Russian Academy of Sciences (HD), Moscow, Russia, prepared from oxidized culture strain CBMC129 ef.</p><p>Isotype. Slide no. 05556a, Herbarium of Addis Ababa University, Addis Ababa, Ethiopia.</p><p>Reference strain. CBMC 129ef from the Culture and Barcode Collection of Microalgae and Cyanobacteria “Algabank” (СВМС), isolated from sample E67.</p><p>Type locality. Ethiopia, Oromia Region, Lake Langano, periphyton, 1591 m elev., 7°38.85’N 38°49.176’E, leg. B.A. Levin, 15 April 2016. Sample E67.</p><p>Description. LM (Figs 1–18). Valves linear-lanceolate with rounded ends. Length 7.4–13.5 μm (11.9 ± 1.6; n=30), width 3.5–4.5 μm (4.0 ± 0.3; n=30). Axial area narrow, weakly expanded towards the central area. Central area bowtie-shaped, formed by 3–4 shortened striae on each side. Raphe filiform. Striae weakly radiate, 24–26 in 10 μm.</p><p>SEM. External views (Figs 19–21). Valve face slightly convex. Raphe weakly lateral. Central raphe ends slightly expanded. In the initial valves, distal raphe ends terminate on valve face. One of the distal raphe ends is curved, the other is almost straight. Striae uniseriate. Shortened intercalary striae and short striae on the valve apices are present. Areolae small, rounded, ca. 70 in 10 μm.</p><p>SEM. Internal views (Figs 22–25). Raphe branches almost straight, filiform. Central raphe ends weakly unilaterally deflected. Distal raphe ends terminating in small helictoglossae. Shortened intercalary striae present. Areolae covered by individual hymenes.</p><p>Sequence data. Partial 18S rDNA gene sequence comprising V4 domain sequence (GenBank accession number PV383267) and partial rbc L sequence (GenBank accession number PV384976) for the strain CBMC129ef.</p><p>Etymology. This species is named after Nikolay Ivanovich Ashinov (1859–1902), a renowned Russian Cossack chieftain and traveler in Africa, who made significant contributions to the development of Russia-Abyssinia relations. He was also an amateur linguist who published the book “The Abyssinian alphabet and the initial Abyssinian-Russian dictionary”.</p><p>Distribution. As yet known only from the type locality.</p><p>Molecular study. S. ashinovii sp. nov. forms an independent branch on the phylogenetic tree (Fig. 26) that is sister to strains of Sellaphora minima (Grunow) D.G. Mann in Round et al. (1990: 552). These strains are part of a larger clade that also includes Sellaphora seminulum (Grunow) D.G. Mann (1989: 2) and S. terrestris Glushchenko, Kezlya, Maltsev &amp; Kulikovskiy in Glushchenko et al. (2022: 2).</p></div>	https://treatment.plazi.org/id/91653536FFA7D15BFF21FCB7FD16FEAC	Public Domain	No known copyright restrictions apply. See Agosti, D., Egloff, W., 2009. Taxonomic information exchange and copyright: the Plazi approach. BMC Research Notes 2009, 2:53 for further explanation.		Plazi	Tseplik, Natalia;Glushchenko, Anton;Maltsev, Yevhen;Genkal, Sergey;Iurmanov, Anton;Kulikovskiy, Maxim	Tseplik, Natalia, Glushchenko, Anton, Maltsev, Yevhen, Genkal, Sergey, Iurmanov, Anton, Kulikovskiy, Maxim (2025): Molecular and morphological investigation of Sellaphora ashinovii sp. nov. and Planothidium paisiusii sp. nov. - two new diatom species from Oromia Region, Ethiopia. Phytotaxa 701 (2): 161-176, DOI: 10.11646/phytotaxa.701.2.3, URL: https://doi.org/10.11646/phytotaxa.701.2.3
91653536FFA2D156FF21F98EFCE9FE50.text	91653536FFA2D156FF21F98EFCE9FE50.taxon	http://purl.org/dc/dcmitype/Text	http://rs.tdwg.org/ontology/voc/SPMInfoItems#GeneralDescription	text/html	en	Planothidium paisiusii Tseplik, Glushchenko, Maltsev, Genkal, Iurmanov & Kulikovskiy 2025	<div><p>Planothidium paisiusii Tseplik, Glushchenko, Maltsev, Genkal, Iurmanov &amp; Kulikovskiy sp. nov. (Figs 27–63)</p><p>Holotype. Slide no. 05583 (represented here by Figure 49), deposited in the Herbarium of K.A. Timiryazev Institute of Plant Physiology, Russian Academy of Sciences (HD), Moscow, Russia, prepared from oxidized culture strain CBMC155 ef.</p><p>Isotype. Slide no. 05583a, Herbarium of Addis Ababa University, Addis Ababa, Ethiopia.</p><p>Reference strain. CBMC 155ef from the Culture and Barcode Collection of Microalgae and Cyanobacteria “Algabank” (СВМС), isolated from sample E14.</p><p>Type locality. Ethiopia, Mesekelhericho, Adaba, West Arsi, nameless mountain stream, periphyton, 2978 m elev., 07°1.96’ N 39°33.277’E., leg. B.A. Levin, 3 April 2016. Sample E14.</p><p>Description. LM (Figs 27–59). Cells in girdle view rectangular, slightly bent. Valves linear-elliptic to linear-lanceolate, with rounded ends. Length 8.6–11.7 μm (9.8 ± 0.6; n=32), width 3.7–4.5 μm (4.1 ± 0.2; n=32). Raphe straight, filiform, with expanded central ends.Axial area on the raphe valve narrow linear, central area may be indistinct, usually asymmetric, formed by 1–2 central striae shortened and spaced wider than the rest. Striae radiate, 15–16 in 10 μm. Axial area on the rapheless valve also linear, widening towards the centre. A rounded cavum is present on the one side of the rapheless valve. Striae weakly radiate to almost parallel in the centre of the valve, more radiate towards the ends, 14–18 in 10 μm.</p><p>SEM. Raphe valve (Figs 60, 62). External central raphe ends straight, expanded, distal ends curved to one side and extended onto the mantle. Internal central raphe ends slightly bent to the opposite sides and set quite widely apart, distal ends terminate in small helictoglossae. Striae multiseriate, each stria consists of 4 (3) rows of small circular areolae.</p><p>Rapheless valve (Figs 61, 63). Cavum large, rounded, usually with a relatively small opening. A vestigial raphe is present on the inside. Striae multiseriate, consisting of 3–4 rows of small circular areolae. Internally, the interstriae are distinctly raised on both valves.</p><p>Sequence data. Partial 18S rDNA gene sequence comprising V4 domain sequence (GenBank accession number PV383266) and partial rbc L sequence (GenBank accession number PV384975) for the strain CBMC155ef.</p><p>Etymology. This species is named after Archimandrite Paisius (Vasily Filippovich Balabanov, 1832–1912), the leader of the spiritual mission of Ashinov’s expeditions to Abyssinia in 1888–1889, honoring his contribution to the strengthening of Russia-Abyssinia ties.</p><p>Distribution. As yet known only from the type locality.</p><p>Molecular study. On the phylogenetic tree (Fig. 64), this species forms a separate branch that is related to strains of the Planothidium frequentissimum (Lange-Bertalot) Lange-Bertalot (1999: 282) and Planothidium victori Novis, Braidwood &amp; Kilroy in Novis et al. (2012: 22) complex.</p></div>	https://treatment.plazi.org/id/91653536FFA2D156FF21F98EFCE9FE50	Public Domain	No known copyright restrictions apply. See Agosti, D., Egloff, W., 2009. Taxonomic information exchange and copyright: the Plazi approach. BMC Research Notes 2009, 2:53 for further explanation.		Plazi	Tseplik, Natalia;Glushchenko, Anton;Maltsev, Yevhen;Genkal, Sergey;Iurmanov, Anton;Kulikovskiy, Maxim	Tseplik, Natalia, Glushchenko, Anton, Maltsev, Yevhen, Genkal, Sergey, Iurmanov, Anton, Kulikovskiy, Maxim (2025): Molecular and morphological investigation of Sellaphora ashinovii sp. nov. and Planothidium paisiusii sp. nov. - two new diatom species from Oromia Region, Ethiopia. Phytotaxa 701 (2): 161-176, DOI: 10.11646/phytotaxa.701.2.3, URL: https://doi.org/10.11646/phytotaxa.701.2.3
91653536FFADD154FF21FF77FCD5FE74.text	91653536FFADD154FF21FF77FCD5FE74.taxon	http://purl.org/dc/dcmitype/Text	http://rs.tdwg.org/ontology/voc/SPMInfoItems#GeneralDescription	text/html	en	Sellaphora ashinovii Tseplik & Glushchenko & Maltsev & Genkal & Iurmanov & Kulikovskiy 2025	<div><p>Sellaphora ashinovii sp. nov.</p><p>Sellaphora ashinovii sp. nov. is morphologically close to S. saugerresii (Desmazières) C.E. Wetzel &amp; D.G. Mann in Wetzel et al. (2015: 209) (see Table 3). These species are similar in valve width (3.5–4.5 μm in S. ashinovii sp. nov. and 3–4 μm in S. saugerresii) and in the shape of the central area (bowtie-shaped, formed by shortened striae). The main differentiating feature between these species is the striae structure: S. ashinovii sp. nov. is characterized by uniseriate striae, while in S. saugerresii the striae are always biseriate. The striae density also differs (24–26 in 10 μm in Sellaphora ashinovii sp. nov. vs. 18–22 in 10 μm in S. saugerresii). Another distinguishing trait is the shape of the axial area which is weakly widening towards the central area in Sellaphora ashinovii sp. nov. and linear in S. saugerresii .</p><p>*derived from published illustrations.</p><p>S. atomoides (Grunow) Wetzel &amp; Van de Vijver in Wetzel et al. (2015: 219) also resembles Sellaphora ashinovii sp. nov. (see Table 3) in valve shape, striae pattern, and structure of central and axial area, the central area being formed by shortened striae in both species. These species can be differentiated by valve width (3.5–4.5 μm in Sellaphora ashinovii sp. nov. vs. 2.6–3.7 μm in S. atomoides) and striae density (24–26 in 10 μm in Sellaphora ashinovii sp. nov. vs. 30–36 in 10 μm in S. atomoides).</p><p>Lastly, Sellaphora ashinovii sp. nov. is similar to S. subseminulum (Hustedt) C.E. Wetzel in Wetzel et al. (2015: 218) (see Table 3). These species resemble each other in valve shape, striae pattern, and shape of axial and central areas. They can be differentiated by valve width (3.5–4.5 μm in Sellaphora ashinovii sp. nov. vs. 3.3–3.8 μm in S. subseminulum). The axial area in the new species is narrow, widening weakly towards the central area, while in S. subseminulum the axial area does not widen (Wetzel et al. 2015, figs 180–182). The central area is bordered by 3–4 shortened striae in Sellaphora ashinovii sp. nov. and by 5–6 very short striae in S. subseminulum (see Table 3). The striae density is 24–26 in 10 μm in Sellaphora ashinovii sp. nov. and 20–24 in 10 μm in S. subseminulum .</p><p>On the phylogenetic tree, the strain of S. ashinovii sp. nov. forms a separate branch that is sister to strains of Sellaphora minima (Fig. 26). Unfortunately, there are no vouchers available for the strain TCC524 of S. minima, thus a morphological comparison with our new species cannot be carried out. Strain BM42, identified as S. cf. minima, was illustrated with a single image in Evans et al. (2008, Fig. 8c), which is also insufficient for a proper morphological comparison. Even so, judging by the one available image, this strain differs from S. ashinovii sp. nov. by valve outline, size of central area, and striae density. Currently, according to Wetzel et al. (2015), S. minima is regarded as a synonym for S. saugerresii which was discussed above.</p><p>Representatives of Sellaphora Mereschkowsky have been fairly well studied with the use of morphological and molecular analysis (Evans et al. 2008; Mann et al. 2009; Vanormelingen et al. 2013, Wetzel et al. 2015), with new species being described in recent years from different parts of the world (Andreeva et al. 2018; Kochoska et al. 2021; Glushchenko et al. 2022; Ni et al. 2022 and others). Nevertheless, acquisition of molecular data from new strains from various geographical locations is essential for correct identification of taxa in floristic and ecological studies, as well as for taxonomical investigations, especially since the genus Sellaphora contains difficult species complexes, e.g. Sellaphora pupula (Mann et al. 2004; Evans et al. 2008, etc.).</p></div>	https://treatment.plazi.org/id/91653536FFADD154FF21FF77FCD5FE74	Public Domain	No known copyright restrictions apply. See Agosti, D., Egloff, W., 2009. Taxonomic information exchange and copyright: the Plazi approach. BMC Research Notes 2009, 2:53 for further explanation.		Plazi	Tseplik, Natalia;Glushchenko, Anton;Maltsev, Yevhen;Genkal, Sergey;Iurmanov, Anton;Kulikovskiy, Maxim	Tseplik, Natalia, Glushchenko, Anton, Maltsev, Yevhen, Genkal, Sergey, Iurmanov, Anton, Kulikovskiy, Maxim (2025): Molecular and morphological investigation of Sellaphora ashinovii sp. nov. and Planothidium paisiusii sp. nov. - two new diatom species from Oromia Region, Ethiopia. Phytotaxa 701 (2): 161-176, DOI: 10.11646/phytotaxa.701.2.3, URL: https://doi.org/10.11646/phytotaxa.701.2.3
91653536FFAED155FF21FDA9FE1BFC7C.text	91653536FFAED155FF21FDA9FE1BFC7C.taxon	http://purl.org/dc/dcmitype/Text	http://rs.tdwg.org/ontology/voc/SPMInfoItems#GeneralDescription	text/html	en	Planothidium paisiusii Tseplik & Glushchenko & Maltsev & Genkal & Iurmanov & Kulikovskiy 2025	<div><p>Planothidium paisiusii sp. nov.</p><p>According to morphological and molecular data, the new species belongs to the genus Planothidium, characterized by a cavum on the rapheless valve, multiseriate striae and straight raphe branches with distal ends turned to one side and extended onto the mantle. The new species can be compared to such known species as P. straubianum Wetzel, Van de Vijver &amp; Ector in Wetzel et al. (2019: 60), P. aueri (Krasske) Lange-Bertalot (1999: 275), and P. curtistriatum Wetzel, Van de Vijver &amp; Ector in Wetzel et al. (2019: 61). P. straubianum (Wetzel et al. 2019) is the most similar to the new species; it can be differentiated by a more linear-elliptic valve outline and a small narrow cavum. The valve dimensions of the two species overlap, but the valves of P. straubianum are generally wider and have a higher maximum length as well (see Table 4). The axial area on the rapheless valve is linear in P. straubianum, more distinctly widened towards the centre in P. paisiusii sp. nov.; the striae on the rapheless valve in P. straubianum consist of 4–5 rows of areolae vs. 3–4 rows in P. paisiusii sp. nov. The outside of the rapheless valve in P. straubianum is ornamented with rounded depressions, while in P. paisiusii sp. nov. there is no ornamentation present.</p><p>P. curtistriatum (Wetzel et al. 2019) is also a small-celled species, however, it has a distinct large axial area on the rapheless valve that distinguishes it from other Planothidium species including P. paisiusii sp. nov. Other differentiating features between P. curtistriatum and the new species are the valve outline which is more lanceolate in P. curtistriatum, coarser striae (11–14 in P. curtistriatum vs. 15–16 on the raphe valve and 14–18 on the rapheless valve in P. paisiusii sp. nov.), and the ornamentation on the rapheless valve (rounded depressions in P. curtistriatum, absent in P. paisiusii sp. nov.).</p><p>P. paisiusii sp. nov. can be compared to smaller valves of P. aueri (Lange-Bertalot et al. 1996; Van de Vijver et al. 2002); there is resemblance in the cavum shape and the structure of the axial area on the rapheless valve. However, the valve outline of P. aueri is different from the new species and the quantitative features such as valve length and width and striae density also allow to differentiate these species (see Table 4): the valves of P. aueri are larger than P. paisiusii sp. nov. and the striae density is higher.</p><p>According to molecular data, P. paisiusii sp. nov. forms a separate branch in a subclade that includes several other species of Planothidium and is part of a larger clade containing Planothidium species. Mostly this subclade is formed by strains of P. victori; the identity of other strains in this subclade was previously discussed in Tseplik et al. (2024b). Our data supports the idea of P. victori actually representing a complex of several cryptic species. Morphologically, P. victori can be differentiated from the new species by its protracted ends, large wide cavum, linear axial area on the rapheless valve, and the ornamentation on the rapheless valve in the form of small round depressions; the quantitative features overlap between the two species, however, the valves of P. paisiusii sp. nov. are generally smaller (maximum length 11.7 μm vs. 19.5 μm in P. victori (Wetzel et al. 2019)) . Planothidium also contains cryptic diversity and several complicated species groups (Jahn et al. 2017; Tseplik et al. 2024 a, b), and accumulation of DNA sequences of new strains is an important asset in resolving the taxonomy of this genus.</p><p>Our study is one of the first molecular studies of diatoms in Africa. Establishing a library of strains from different locations is a very important step in diatom research, and data like this is valuable for future studies in taxonomy, biodiversity, and biomonitoring.</p></div>	https://treatment.plazi.org/id/91653536FFAED155FF21FDA9FE1BFC7C	Public Domain	No known copyright restrictions apply. See Agosti, D., Egloff, W., 2009. Taxonomic information exchange and copyright: the Plazi approach. BMC Research Notes 2009, 2:53 for further explanation.		Plazi	Tseplik, Natalia;Glushchenko, Anton;Maltsev, Yevhen;Genkal, Sergey;Iurmanov, Anton;Kulikovskiy, Maxim	Tseplik, Natalia, Glushchenko, Anton, Maltsev, Yevhen, Genkal, Sergey, Iurmanov, Anton, Kulikovskiy, Maxim (2025): Molecular and morphological investigation of Sellaphora ashinovii sp. nov. and Planothidium paisiusii sp. nov. - two new diatom species from Oromia Region, Ethiopia. Phytotaxa 701 (2): 161-176, DOI: 10.11646/phytotaxa.701.2.3, URL: https://doi.org/10.11646/phytotaxa.701.2.3
