identifier	taxonID	type	CVterm	format	language	title	description	additionalInformationURL	UsageTerms	rights	Owner	contributor	creator	bibliographicCitation
985F8788FFF7FC5602CB0E7EC0250618.text	985F8788FFF7FC5602CB0E7EC0250618.taxon	http://purl.org/dc/dcmitype/Text	http://rs.tdwg.org/ontology/voc/SPMInfoItems#GeneralDescription	text/html	en	Dna	<div><p>DNA Extraction, PCR Amplification and Sequencing</p><p>Fungal mycelium was scraped from the surface of colonies grown on potato dextrose agar (PDA), transferred into a 1.5 mL centrifuge tube. The TreliefTM Plant Genomic DNA Kit (TSP101-50) (Beijing Tsingke Biological Engineering Technology and Services Co., Ltd, Beijing, P.R. China) was used to extract DNA from the ground mycelium according to the manufacturer’s instructions. Three gene regions: ITS, LSU, SSU were amplified using ITS5/ITS4, LR0R/LR5, NS1/NS4 (Vilgalys, 1990, White et al. 1990, Liu et al. 1999). The amplification was performed in a 25 μL reaction volume containing 9.5 μL deionized water, 12.5 μL 2 × Taq PCR Master Mix with blue dye (Sangon Biotech, China), 1 μL of DNA template and 1 μL of each primer (10 μM). The amplification condition for ITS, LSU, SSU were followed Luo et al. (2018). PCR amplification was confirmed on 1% agarose electrophoresis gels stained with ethidium bromide. Purification and sequencing of PCR products were sent for sequencing at Tsingke Biological Engineering Technology and Services Company, Yunnan, China.</p></div>	https://treatment.plazi.org/id/985F8788FFF7FC5602CB0E7EC0250618	Public Domain	No known copyright restrictions apply. See Agosti, D., Egloff, W., 2009. Taxonomic information exchange and copyright: the Plazi approach. BMC Research Notes 2009, 2:53 for further explanation.		Plazi	Cai, Yuan-Ting;Zhang, Liang;Shen, Hong-Wei;Bao, Dan-Feng;Luo, Zong-Long	Cai, Yuan-Ting, Zhang, Liang, Shen, Hong-Wei, Bao, Dan-Feng, Luo, Zong-Long (2025): Setophoma aseptata sp. nov. and new record of Minutisphaera aspera from Yuanjiang River Basin, China. Phytotaxa 702 (3): 287-299, DOI: 10.11646/phytotaxa.702.3.4, URL: https://doi.org/10.11646/phytotaxa.702.3.4
985F8788FFF3FC5202CB0D66C1A807A8.text	985F8788FFF3FC5202CB0D66C1A807A8.taxon	http://purl.org/dc/dcmitype/Text	http://rs.tdwg.org/ontology/voc/SPMInfoItems#GeneralDescription	text/html	en	Setophoma aseptata , Y. T. Cai, L. Zhang & Z. L. Luo 2025	<div><p>Setophoma aseptata, Y.T. Cai, L. Zhang &amp; Z.L. Luo, sp. nov. FIGURE 1</p><p>Fungal Names number: FN572474</p><p>Etymology:— aseptata, referring to the aseptate conidia of this fungus.</p><p>Holotype:— KUN-HKAS 146438</p><p>Saprobic on decaying submerged wood in freshwater habitat. Asexual morph: Conidiomata 88–106 × 110–140 µm, scattered or gregarious, globose to subglobose, glabrous, papillate, with a small pore-like opening at the center, dark brown to black. Conidiomata wall 17–36 μm thick, consisting of multiple-layers of thick-walled; dark brown cells composed of textura angularis cells in the outer wall, and rectangular hyaline cells in inner the wall. Conidiophores reduced to conidiogenous cells. Conidiogenous cells monophialidic, hyaline, smooth-walled, cylindric-clavate to ampulliform, 5–11 × 1–5 µm (x = 7 × 2.5 μm, n = 20). Conidia 5–6.5 × 3.5–4 µm (x = 5.7 × 3.8 μm, n = 40), solitary, hyaline, ovoid or ellipsoidal, smooth and thin-walled, aseptate, guttulate. Sexual morph: Undetermined.</p><p>Culture characteristics: Conidia germinated on PDA medium and germ tubes produced from both ends within 12 h. Colonies grew on PDA, reaching approximately 30 mm in diameter after three weeks at 25°C, colony edge appears irregularly circular, flat, felty, with dense, dark brown mycelium on the surface, with a dark brown elevation in the center; in reverse, brown at the entire margin.</p><p>Material examined:— CHINA, Yunnan Province, Yuxi City, on submerged decaying wood in freshwater stream in Yuanjiang River Basin, February 2022, Hong-Wei Shen, H-833 (KUN-HKAS 146438, holotype), ex-type living culture, CGMCC 3.27726 = KUNCC 24-17665.</p><p>Notes: Phylogenetic analyses revealed that Setophoma aseptata (CGMCC 3.27726) formed a cluster with S. yunnanensis (CGMCC 3.19529 and LC 6753) and S. yingyisheniae (CGMCC 3.19527 and LC 13479) (Figure 2). Nucleotide comparison of ITS showed that S. aseptata differs from S. yunnanensis by 28 base pairs out of 508 (5.51%) and from S. yingyisheniae by 41 base pairs out of 509 (8.06%). Setophoma aseptata shows both morphological similarities and differences when compared with S. yunnanensis and S. yingyisheniae (Liu et al. 2019) . It shares with S. yunnanensis the trait of having hyaline, smooth, globose, and aseptate conidia. Nevertheless, S. aseptata can be distinguished from S. yunnanensis by its larger conidia (5–6.5 × 3.5–4 µm vs. 3.5–5 × 2–3 µm). Similarly, S. aseptata and S. yingyisheniae both possess dark-brown or black, globose or sub-globose conidiomata with an apical opening. However, it differs from S. yingyisheniae by lacking setae. Therefore, based on morphological characteristics and phylogenetic analyses, we introduce Setophoma aseptata as a new species.</p></div>	https://treatment.plazi.org/id/985F8788FFF3FC5202CB0D66C1A807A8	Public Domain	No known copyright restrictions apply. See Agosti, D., Egloff, W., 2009. Taxonomic information exchange and copyright: the Plazi approach. BMC Research Notes 2009, 2:53 for further explanation.		Plazi	Cai, Yuan-Ting;Zhang, Liang;Shen, Hong-Wei;Bao, Dan-Feng;Luo, Zong-Long	Cai, Yuan-Ting, Zhang, Liang, Shen, Hong-Wei, Bao, Dan-Feng, Luo, Zong-Long (2025): Setophoma aseptata sp. nov. and new record of Minutisphaera aspera from Yuanjiang River Basin, China. Phytotaxa 702 (3): 287-299, DOI: 10.11646/phytotaxa.702.3.4, URL: https://doi.org/10.11646/phytotaxa.702.3.4
985F8788FFF1FC5D02CB0CCAC6B30688.text	985F8788FFF1FC5D02CB0CCAC6B30688.taxon	http://purl.org/dc/dcmitype/Text	http://rs.tdwg.org/ontology/voc/SPMInfoItems#GeneralDescription	text/html	en	Minutisphaera aspera Raja, Oberlies, Shearer, A. N. Mill., Mycologia	<div><p>Minutisphaera aspera Raja, Oberlies, Shearer, A. N. Mill., Mycologia 107(4): 855 (2015). FIGURE 3</p><p>MycoBank: MB811063</p><p>Saprobic on decaying wood, submerged in freshwater habitats. Asexual morph: Undetermined. Sexual morph: Ascomata 91–189 µm, superficial, scattered, dark brown to black, membranous. Ostiole absent. Peridium 2–5 µm wide, comprises two layers, outer layers composed of dark brown cells of textura angularis, inner layer composed hyaline cells of textura prismatica. Hamathecium composed of cellular pseudoparaphyses, sparse in young ascomata, becoming abundant with age, hyaline, septate, filamentous. Asci 59–86 × 27–40 µm (= 70 × 34 µm, n = 20), 8-spored, fissitunicate, obovoid to broadly cylindrical, thick-walled at the apex, lacks an ocular chamber. Ascospores 20–26 × 11–15 µm (= 23 × 14 µm, n = 30), multi-guttulate, bitunicate, ellipsoidal, straight, with a median septum, constrict at the septum, hyaline and thin when young, becoming olive to dark brown and rough-walled at mature, upper and lower cells are equal in length and width, with or without sheath.</p><p>Culture characteristics: Ascospores germinating on PDA within 12 h and germ tubes produced from the one end of the spore. Colonies grew on PDA, reaching 18–27 mm in three weeks at 25°C, with dense mycelia, dry, colony edge appears irregularly circular, center is gray velvety with dark brown fuzzy protrusions on the sides, dark brown from reverse.</p><p>Material examined:— CHINA, Yunnan Province, Yuxi City, on submerged decaying wood in freshwater stream in Yuanjiang River Basin, February 2022, Hong-Wei Shen, S- 3749 (KUN-HKAS 135204), living culture, CGMCC 3.27728 = KUNCC 24-17898.</p><p>Notes: In phylogenetic analyses, our new collection (CGMCC 3.27728) clustered with Minutisphaera aspera (G 427 1a and G 427 1b) with 99% ML and 1.00 PP support (FIGURE 4). Nucleotide comparison showed that our fungal isolate (CGMCC 3.27728) differs from M. aspera (G 427 1a) by 9 base pairs out of 538 in the ITS region (1.67%), 1 base pair out of 767 in the LSU region (0.13%), 1 base pair out of 993 in the SSU region (0.10%). Morphologically, our new isolate is similar to M. aspera in having dark brown to black ascomata, obovoid, fissitunicate asci and fusiform to ellipsoidal, hyaline to dark brown, 1-septate, verruculose, guttulate ascospores with similar size (20–26 × 11–15 µm vs. 24–33 × 9–14 µm). Thus, we identify our new collection as M. aspera, a new report for China.</p></div>	https://treatment.plazi.org/id/985F8788FFF1FC5D02CB0CCAC6B30688	Public Domain	No known copyright restrictions apply. See Agosti, D., Egloff, W., 2009. Taxonomic information exchange and copyright: the Plazi approach. BMC Research Notes 2009, 2:53 for further explanation.		Plazi	Cai, Yuan-Ting;Zhang, Liang;Shen, Hong-Wei;Bao, Dan-Feng;Luo, Zong-Long	Cai, Yuan-Ting, Zhang, Liang, Shen, Hong-Wei, Bao, Dan-Feng, Luo, Zong-Long (2025): Setophoma aseptata sp. nov. and new record of Minutisphaera aspera from Yuanjiang River Basin, China. Phytotaxa 702 (3): 287-299, DOI: 10.11646/phytotaxa.702.3.4, URL: https://doi.org/10.11646/phytotaxa.702.3.4
