taxonID	type	description	language	source
985F8788FFF3FC5202CB0D66C1A807A8.taxon	etymology	Etymology: — aseptata, referring to the aseptate conidia of this fungus.	en	Cai, Yuan-Ting, Zhang, Liang, Shen, Hong-Wei, Bao, Dan-Feng, Luo, Zong-Long (2025): Setophoma aseptata sp. nov. and new record of Minutisphaera aspera from Yuanjiang River Basin, China. Phytotaxa 702 (3): 287-299, DOI: 10.11646/phytotaxa.702.3.4, URL: https://doi.org/10.11646/phytotaxa.702.3.4
985F8788FFF3FC5202CB0D66C1A807A8.taxon	materials_examined	Holotype: — KUN-HKAS 146438 Saprobic on decaying submerged wood in freshwater habitat. Asexual morph: Conidiomata 88 – 106 × 110 – 140 µm, scattered or gregarious, globose to subglobose, glabrous, papillate, with a small pore-like opening at the center, dark brown to black. Conidiomata wall 17 – 36 μm thick, consisting of multiple-layers of thick-walled; dark brown cells composed of textura angularis cells in the outer wall, and rectangular hyaline cells in inner the wall. Conidiophores reduced to conidiogenous cells. Conidiogenous cells monophialidic, hyaline, smooth-walled, cylindric-clavate to ampulliform, 5 – 11 × 1 – 5 µm (x = 7 × 2.5 μm, n = 20). Conidia 5 – 6.5 × 3.5 – 4 µm (x = 5.7 × 3.8 μm, n = 40), solitary, hyaline, ovoid or ellipsoidal, smooth and thin-walled, aseptate, guttulate. Sexual morph: Undetermined. Culture characteristics: Conidia germinated on PDA medium and germ tubes produced from both ends within 12 h. Colonies grew on PDA, reaching approximately 30 mm in diameter after three weeks at 25 ° C, colony edge appears irregularly circular, flat, felty, with dense, dark brown mycelium on the surface, with a dark brown elevation in the center; in reverse, brown at the entire margin. Material examined: — CHINA, Yunnan Province, Yuxi City, on submerged decaying wood in freshwater stream in Yuanjiang River Basin, February 2022, Hong-Wei Shen, H- 833 (KUN-HKAS 146438, holotype), ex-type living culture, CGMCC 3.27726 = KUNCC 24 - 17665. Notes: Phylogenetic analyses revealed that Setophoma aseptata (CGMCC 3.27726) formed a cluster with S. yunnanensis (CGMCC 3.19529 and LC 6753) and S. yingyisheniae (CGMCC 3.19527 and LC 13479) (Figure 2). Nucleotide comparison of ITS showed that S. aseptata differs from S. yunnanensis by 28 base pairs out of 508 (5.51 %) and from S. yingyisheniae by 41 base pairs out of 509 (8.06 %). Setophoma aseptata shows both morphological similarities and differences when compared with S. yunnanensis and S. yingyisheniae (Liu et al. 2019). It shares with S. yunnanensis the trait of having hyaline, smooth, globose, and aseptate conidia. Nevertheless, S. aseptata can be distinguished from S. yunnanensis by its larger conidia (5 – 6.5 × 3.5 – 4 µm vs. 3.5 – 5 × 2 – 3 µm). Similarly, S. aseptata and S. yingyisheniae both possess dark-brown or black, globose or sub-globose conidiomata with an apical opening. However, it differs from S. yingyisheniae by lacking setae. Therefore, based on morphological characteristics and phylogenetic analyses, we introduce Setophoma aseptata as a new species.	en	Cai, Yuan-Ting, Zhang, Liang, Shen, Hong-Wei, Bao, Dan-Feng, Luo, Zong-Long (2025): Setophoma aseptata sp. nov. and new record of Minutisphaera aspera from Yuanjiang River Basin, China. Phytotaxa 702 (3): 287-299, DOI: 10.11646/phytotaxa.702.3.4, URL: https://doi.org/10.11646/phytotaxa.702.3.4
985F8788FFF1FC5D02CB0CCAC6B30688.taxon	description	Saprobic on decaying wood, submerged in freshwater habitats. Asexual morph: Undetermined. Sexual morph: Ascomata 91 – 189 µm, superficial, scattered, dark brown to black, membranous. Ostiole absent. Peridium 2 – 5 µm wide, comprises two layers, outer layers composed of dark brown cells of textura angularis, inner layer composed hyaline cells of textura prismatica. Hamathecium composed of cellular pseudoparaphyses, sparse in young ascomata, becoming abundant with age, hyaline, septate, filamentous. Asci 59 – 86 × 27 – 40 µm (= 70 × 34 µm, n = 20), 8 - spored, fissitunicate, obovoid to broadly cylindrical, thick-walled at the apex, lacks an ocular chamber. Ascospores 20 – 26 × 11 – 15 µm (= 23 × 14 µm, n = 30), multi-guttulate, bitunicate, ellipsoidal, straight, with a median septum, constrict at the septum, hyaline and thin when young, becoming olive to dark brown and rough-walled at mature, upper and lower cells are equal in length and width, with or without sheath. Culture characteristics: Ascospores germinating on PDA within 12 h and germ tubes produced from the one end of the spore. Colonies grew on PDA, reaching 18 – 27 mm in three weeks at 25 ° C, with dense mycelia, dry, colony edge appears irregularly circular, center is gray velvety with dark brown fuzzy protrusions on the sides, dark brown from reverse.	en	Cai, Yuan-Ting, Zhang, Liang, Shen, Hong-Wei, Bao, Dan-Feng, Luo, Zong-Long (2025): Setophoma aseptata sp. nov. and new record of Minutisphaera aspera from Yuanjiang River Basin, China. Phytotaxa 702 (3): 287-299, DOI: 10.11646/phytotaxa.702.3.4, URL: https://doi.org/10.11646/phytotaxa.702.3.4
985F8788FFF1FC5D02CB0CCAC6B30688.taxon	materials_examined	Material examined: — CHINA, Yunnan Province, Yuxi City, on submerged decaying wood in freshwater stream in Yuanjiang River Basin, February 2022, Hong-Wei Shen, S- 3749 (KUN-HKAS 135204), living culture, CGMCC 3.27728 = KUNCC 24 - 17898. Notes: In phylogenetic analyses, our new collection (CGMCC 3.27728) clustered with Minutisphaera aspera (G 427 1 a and G 427 1 b) with 99 % ML and 1.00 PP support (FIGURE 4). Nucleotide comparison showed that our fungal isolate (CGMCC 3.27728) differs from M. aspera (G 427 1 a) by 9 base pairs out of 538 in the ITS region (1.67 %), 1 base pair out of 767 in the LSU region (0.13 %), 1 base pair out of 993 in the SSU region (0.10 %). Morphologically, our new isolate is similar to M. aspera in having dark brown to black ascomata, obovoid, fissitunicate asci and fusiform to ellipsoidal, hyaline to dark brown, 1 - septate, verruculose, guttulate ascospores with similar size (20 – 26 × 11 – 15 µm vs. 24 – 33 × 9 – 14 µm). Thus, we identify our new collection as M. aspera, a new report for China.	en	Cai, Yuan-Ting, Zhang, Liang, Shen, Hong-Wei, Bao, Dan-Feng, Luo, Zong-Long (2025): Setophoma aseptata sp. nov. and new record of Minutisphaera aspera from Yuanjiang River Basin, China. Phytotaxa 702 (3): 287-299, DOI: 10.11646/phytotaxa.702.3.4, URL: https://doi.org/10.11646/phytotaxa.702.3.4
