identifier	taxonID	type	CVterm	format	language	title	description	additionalInformationURL	UsageTerms	rights	Owner	contributor	creator	bibliographicCitation
E707878A6D650F6EFF0C8417335DF9FD.text	E707878A6D650F6EFF0C8417335DF9FD.taxon	http://purl.org/dc/dcmitype/Text	http://rs.tdwg.org/ontology/voc/SPMInfoItems#GeneralDescription	text/html	en	Dna extraction PCR	<div><p>DNA extraction, PCR amplification, and sequencing</p><p>Genomic DNA was extracted from c. 20 mg of dried plant material using DNA extraction kit (Sinaclon Co., Iran) based on CTAB protocol following the manufacturer’s instructions. For PCR amplification of nrDNA ITS and ETS regions, the pair primers ‘N-nc18S10’ and ‘C26A’ (Wen &amp; Zimmer 1996); and ‘18S-ETS’ and ‘Umb-ETS’ (Logacheva et al. 2010) were used respectively.</p><p>The PCR was performed using ready-to-use MasterMix PCR Kit following the protocol. Details of the PCR ITS and ETS amplification regions were described previously (Logacheva et al. 2010, Panahi 2023). To check the PCR products, an aliquot of the reaction sample was electrophoresed in a 1% agarose gel based on a TAE buffer and stained with the DNA-safe stain. The successful PCR products were selected and with the same primers were used for subsequent cycle sequencing. Cycle sequencing was performed by automated sequencing using Big Dye terminators (Applied Biosystems-ABI) in the laboratories of MomGene Inc. in Tehran. Both DNA strands across the entire DNA regions were sequenced to avoid ambiguity in base determination. The chromatographs were assembled and edited using SeqMan Pro ver. 12 (Dnastar, Madison, WI, USA). Newly obtained sequences were deposited in the GenBank (Appendix 1).</p></div>	https://treatment.plazi.org/id/E707878A6D650F6EFF0C8417335DF9FD	Public Domain	No known copyright restrictions apply. See Agosti, D., Egloff, W., 2009. Taxonomic information exchange and copyright: the Plazi approach. BMC Research Notes 2009, 2:53 for further explanation.		Plazi	Panahi, Mehrnoush;Lyskov, Dmitry	Panahi, Mehrnoush, Lyskov, Dmitry (2025): Leutea kurdistanica is a new synonym of Sclerochorton haussknechtii (Apiaceae), a new member of the Cachrys clade. Phytotaxa 693 (1): 55-66, DOI: 10.11646/phytotaxa.693.1.3, URL: https://doi.org/10.11646/phytotaxa.693.1.3
E707878A6D650F6EFF0C826B3368FC3D.text	E707878A6D650F6EFF0C826B3368FC3D.taxon	http://purl.org/dc/dcmitype/Text	http://rs.tdwg.org/ontology/voc/SPMInfoItems#GeneralDescription	text/html	en	Dna sampling	<div><p>Taxon and DNA sampling</p><p>In this research, two specimens of Leutea kurdistanica (including holotype) were investigated using molecular and morphological features and compared with other species of Leutea . To determine its relationships to tribes and clades of Apioideae, previously available ITS (116 taxa) and ETS (55 taxa) sequences belonging to various taxa of Apioideae in each related tribe were retrieved from GenBank. The voucher specimens and GenBank accession numbers of the species are given in Appendix 1. The sequences of 10 new accessions for both ITS and ETS regions were obtained in this study (voucher information and GenBank references are given in Appendix 1). The outgroup in the ITS data matrix was assigned to two species of Bupleurum Linnaeus (1753: 236), and Physospermum cornubiense (Linnaeus 1756: 13) Candolle (1830: 246) was used as the outgroup in the ETS data matrix based on the results of phylogenetic studies of the subfamily Apioideae and pollen traits in the early-diverging Apioideae (Banasiak et al. 2013, Baczynski et al. 2021).</p></div>	https://treatment.plazi.org/id/E707878A6D650F6EFF0C826B3368FC3D	Public Domain	No known copyright restrictions apply. See Agosti, D., Egloff, W., 2009. Taxonomic information exchange and copyright: the Plazi approach. BMC Research Notes 2009, 2:53 for further explanation.		Plazi	Panahi, Mehrnoush;Lyskov, Dmitry	Panahi, Mehrnoush, Lyskov, Dmitry (2025): Leutea kurdistanica is a new synonym of Sclerochorton haussknechtii (Apiaceae), a new member of the Cachrys clade. Phytotaxa 693 (1): 55-66, DOI: 10.11646/phytotaxa.693.1.3, URL: https://doi.org/10.11646/phytotaxa.693.1.3
