2.2. Characterization of TlTPS820 expressed in N. benthamiana

Leaves from N. benthamianaexpressing Tl TPS820 were extracted with hexane and analyzed by GC-MS. The analysis yielded a sesquiterpenoid profile identical to Tg TPS2 (Fig. 1). The main product of Tg TPS2 is epikunzeaol (1) (Fig. 2), thus Tl TPS820 is also an epikunzeaol synthase and an orthologue of Tg TPS2. As described previously, the high injection port temperature at 250 ̊C lead to cope-rearrangement of 1 (Andersen et al., 2017). Thus, 1 was partly degraded to two unknown alcohols .