Key to species of Agenamyia Albuquerque [adapted from de Carvalho et al. (2023)]

1 Male with prolongation of the wing membrane between R 2 +3 and M veins [Figs 2a, 2c and Fig. 13a in de Carvalho et al. (2023)]............................................................................................. 2

- Males without prolongation of the wing membrane between R 2+3 and M veins [Figs 13b–c in de Carvalho et al. (2023)].... 3

2 Mesonotum with two slightly differentiated median grey stripes [Fig. 2c in de Carvalho et al. (2023)]; legs entirely dark brown; sternite 5 with four setae of each side [Fig. 3a in de Carvalho et al. (2023)]; epand elongated in lateral view [Fig. 3b in de Carvalho et al. (2023)]; cerc with divergent apex in posterior view [Fig. 3c in de Carvalho et al. (2023)]............................................................................ A. colombiana de Carvalho, Wolff & Fogaça, 2023

- Mesonotum dark brown, with scattered brown spots (Figs 2, 4); legs dark brown except mid and hind tibiae yellowish brown; sternite 5 with more than four setae of each side (Figs 7–8); epand rounded in lateral view (Figs 9–10); cerc with parallel apex in posterior view (Figs 11–12)............................................................. A. diversa sp. nov.

3 First postsutural dorsocentral seta smaller than the other setae; cerc with an almost parallel apex [Fig. 9c in de Carvalho et al. (2023)]............................................................................................. 4

- Three postsutural dorsocentral subequal setae; cerc with divergent apex [Fig. 3c in de Carvalho et al. (2023)]............ 5

4 Vein M slightly curved before the tip of the wing; tip of the wing with conspicuous cloudy brown spots [Fig. 13b in de Carvalho et al. (2023)]................................................... A. maculata de Carvalho, Wolff & Fogaça, 2023

- Vein M straight; tip of the wing without a conspicuous brown spot [Fig. 5c in de Carvalho et al. (2023)]....................................................................................... A. exotica de Carvalho & Couri, 1992

5 Syntergite 1+2 translucid [Figs 6a, c in de Carvalho et al. (2023)]; hind femur with two apical av setae; cerc with several setulae; very short distph [Figs 7c in de Carvalho et al. (2023); and Fig. 11 in Albuquerque (1953)].............................................................................................. A. fumipennis Albuquerque, 1953

- Syntergite 1+2 not translucid [Fig. 11c in de Carvalho et al. (2023)]; hind femur on av with three setae on apical half; cerc with three pairs of setulae [Fig. 12c in de Carvalho et al. (2023)]; reduced distph [Fig. 12d in de Carvalho et al. (2023)]........................................................................ A. timida de Carvalho, Wolff & Fogaça, 2023 .