Nannizzia sinensis Zhi.Y. Zhang, Y.F. Han & Z.Q. Liang sp. nov.

Fig. 13

Etymology.

Refers to the country where this fungus was first isolated.

Type.

China: Guizhou Province, Zunyi City, the affiliated hospital of Zunyi Medical University 27°70'79"N, 106°94'54"E, soil, 5 Jul 2016, Z.Y. Zhang (HMAS 351892 holotype designated here, ex-type living culture CGMCC 3.20873 = GZUIFR 22.012) .

Description.

Culture characteristics (14 d at 25 °C): Colony on PDA 76-79 mm diam., yellowishwhite (4A2), floccose, fluffy, edge entire to diffuse; reverse white (4A1). Colony on MEA 51-52 mm diam., yellowish-white (4A2), floccose, wavy from centre to margin, edge entire; reverse white (6A1) to brownish-orange (6C8) from margin to centre. Colony on OA 52-54 mm diam., white (1A1), fluffy, sparse at the centre, margin irregular; reverse white (1A1).

Hyphae hyaline, septate, smooth, branched, 1.0-4.5 μm wide; racquet hyphae and spiral hyphae not observed. Macroconidia abundant, thin- or moderately thick-walled, smooth-walled or slightly verrucose, borne individually on short branches alongside hyphae or complex branched conidiophores, fusiform, 1-5-septate, 45.0-51.0 × 11.5-12.5 μm (av. 48.6 × 12.3 μm, n = 50). Microconidia scant, sessile or short-stalked, aseptate, smooth-walled. Two types of microconidia are present: subspherical to spherical, 5.0-9.0 × 5.0-8.0 μm (av. 8.2 × 6.8 μm, n = 50); obovoidal or clavate, 3.5-6.5 × 2.0-2.5 μm (av. 5.4 × 2.3 μm, n = 50). Sexual morph not observed.

Additional specimens examined.

China: Hainan Province, Haikou City, Haidian Campus of Hainan University 20°05'76"N, 110°32'91"E, soil, 28 Aug 2019, Z.Y. Zhang, GZUIFR 22.054 ; Jiangxi Province, Jian City, Jinggangshan University, soil, 22 Aug 2019, Z.Y. Zhang, GZUIFR 22.055, ibid., GZUIFR 22.056 .

Notes.

In the multi-locus phylogenetic analysis, our four isolates form a distinct clade and are closely related to N. aenigmatica, N. gypsea and N. lorica (Fig. 12). However, N. sinensis can be distinguished from N. aenigmatica by the presence of macroconidia (Dukik et al. 2020). In addition, N. sinensis differs from N. gypsea and N. lorica by the presence of subspherical to spherical microconidia (Dukik et al. 2020).