Microdochium sinense S. B. Liu, X. Y. Liu, Z. Meng & X. G. Zhang, J. Fungi 2022, 8, 577.

Fig. 6

Material examined.

China, Hainan Province, Jianfengling National Forest Park, on diseased leaves of Phragmites australis, 12 April 2023, Z. X. Zhang (HSAUP 3922-1), living culture SAUCC 3922-1; ibid., (HSAUP 3922-3), living culture SAUCC 3922-3.

Description.

Endogenic on diseased leaves of Phragmites australis . Mycelia are superficial and immersed, 2.1–2.9 µm wide, branched, membranous and hyaline. Conidia are solitary, hyaline, straight, oblong to ellipsoid, 12.3–15 × 3.5–5.6 µm, multi-guttulate, apex rounded, base usually flattened. Conidiophores were not observed, chlamydospores were not observed, sexual morphs were not observed, see Fig. 6.

Culture characteristics.

Cultures incubated on PDA at 25 ° C in darkness, reach-ing 72–76 mm diam., had a growth rate of 5.1–5.4 mm / day after 14 days, with moder-ate aerial mycelia, milky white to grey-white, with irregular margin, reverses light brown in the centre, with grey-white and regular margin.

Notes.

Phylogenetic analyses of four combined genes (ITS, LSU, RPB 2 and TUB 2) showed that SAUCC 3922-1 and SAUCC 3922-3 clustered with the type collection of Microdochium sinense with strong support (Fig. 1). We, therefore, identified the isolated strains (SAUCC 3922-1 and SAUCC 3922-3) as Mi. sinense . Morphologically, the conidia of the both (newly isolated and type) were similar (12.3–15 × 3.5–5.6 vs. 11.5–19.34 × 2.8–5.4 µm).