Samsoniella haniana Hong Yu bis, Yao Wang & Z.Q. Wang, in Wang, Wang, Dong, Fan, Dao & Yu, Journal of Fungi 8: 20, 2022

Fig. 5

Description.

Synnemata arising from every part of the body of the pupa host. Synnemata erect, usually irregularly branched at the apex, Isaria -like morph producing a mass of conidia at the branch apex, powdery and floccose. Colonies on PDA, attaining a diameter of 32-35 mm after 14 days at 25 °C, white, consisting of a basal felt, floccose hyphal overgrowth; reverse yellowish. Hyphae septate, hyaline, smooth-walled, 1.3-1.8 μm wide. Conidiophores hyaline, smooth-walled, with single phialide or whorls of 2-8 phialides or verticillium-like from hyphae directly, 16.1-23.9 × 1.7-2.2 μm . Phialides consisting of a cylindrical to ellipsoidal, somewhat inflated base, 5.0-6.9 × 1.8-2.5 μm, tapering to a thin neck. Conidia hyaline, smooth-walled, fusiform to subglobose, 1.7-3.4 × 1.7-2.1 μm, forming divergent and basipetal chains. Sexual state not observed.

Host.

Pupa ( Lepidoptera).

Material examined.

China, Guizhou, Qiannan Buyei and Miao Autonomous Prefecture, Duyun City, Mayao River Valley (26°22'8.3748"N, 107°23'16.96"E). On a pupa ( Lepidoptera), buried in soil, 4 September 2021, Wanhao Chen, GZAC DY09103 (specimen), DY091031, DY091032 (living culture); On a ladybug ( Coccinellidae); On the moss, 4 September 2021, Wanhao Chen, GZAC DY09102 (specimen), DY091021, DY091022 (living culture); On a caterpillar ( Lepidoptera), buried in soil, 4 September 2021, Wanhao Chen, GZAC DY09115 (specimen), DY091151, DY091152 (living culture); On a pupa ( Lepidoptera), buried in soil, 4 September 2021, Wanhao Chen, GZAC DY0929, DY09158 (specimen) .

Remarks.

Strains DY091021, DY091022, DY091031, DY091032, DY091151 and DY091152 were identified as belonging to Samsoniella, based on the BLASTn result and the phylogenetic analyses (Fig. 1) and clustered with S. haniana in a subclade with high statistical support (100% ML/ 1 PP). The characteristics of those strains were very closely linked with S. haniana, which had fusiform or oval conidia (2.3-3.7 × 1.2-2.8 μm), phialide (5.4-12.1 × 1.2-2.9 μm) and a pupa host. Thus, the molecular phylogenetic results and morphologically-based conclusions supported the idea that strains DY091021, DY091022, DY091031, DY091032, DY091151 and DY091152 were S. haniana .