Teunia qingyuanensis Y. Z. Qiao & F. L. Hui sp. nov.

Fig. 3 B

Etymology.

Referring to the locality, Qingyuan Mountain, where the type strain was collected.

Typus.

China. Fujian Prov. Quanzhou City, Qingyuan Mountain; in rotting wood; Mar 2022; W. T. Hu & S. B. Chu; NYNU 22475 (holotype GDMCC 2.294 T, culture ex-type PYCC 9929; GenBank Nos: OP 269841, OP 269842, PP 236728) .

Description.

On YM agar after seven days at 20 ° C, the streak culture was cream, mucoid and smooth, with an entire margin. After seven days in YM broth at 20 ° C, single cells were ovoid to ellipsoidal, 3–7 × 4–7.5 μm, budding polar. After one month at 20 ° C, sediment was present. In Dalmau plate culture on CMA, no hyphae or pseudohyphae were formed. Sexual structures were not observed on PDA, CMA or V 8 agar. Glucose fermentation was absent. Glucose, inulin, sucrose, raffinose, melibiose, galactose, lactose, trehalose, maltose, melezitose, cellobiose, salicin, L-sorbose (weak), L-rhamnose, D-xylose, L-arabinose, D-arabinose, 5 - keto-D-gluconate, D-ribose, ethanol, glycerol, ribitol, galactitol, D-mannitol, D-glucitol, myo-inositol, DL-lactate, succinate, D-gluconate, 2 - keto-D-gluconate, D-glucuronate, and glucono- 1, 5 - lactone were assimilated as carbon sources; methanol, erythritol, and D-glucosamine were not assimilated. Ethylamine and L-lysine were assimilated as nitrogen sources; nitrate, nitrite, and cadaverine were not assimilated. Maximum growth temperature was 30 ° C. Growth in vitamin-free medium was positive. Growth on 50 % (w / w) glucose-yeast extract agar was negative. Starch-like substances were not produced. Urease activity and Diazonium Blue B reaction were positive.

Note.

In the molecular analysis (Fig. 2), T. qingyuanensis sp. nov. formed a distinct clade together with T. cuniculi . They can be differentiated by the ability of T. qingyuanensis sp. nov. to grow in vitamin-free medium and to assimilate raffinose, melibiose, and DL-Lactate.