Ophiomyia versera Guglya

Guglya, Yuliia, 2021, Rearing mining flies (Diptera: Agromyzidae) from host plants as an instrument for associating females with males, with the description of seven new species, Zootaxa 5014 (1), pp. 1-158 : 19

Ophiomyia versera Guglya

( Figs. 41–44 , 341–348 )

Material examined: Ukraine: Kyiv Region: Kyiv , location “Lysa Hora”, 50°23’N, 30°32’E, 8, 9.viii.2019, Yu. Guglya, ex Campanula rapunculoides (1♂ 1♀) .

Host. Campanulaceae : Campanula rapunculoides L. (new record).

Mine. ( Fig. 41 ) The larva feeds solitarily in a elongate blotch, lobate mine on the upper side of the leaf. Pupation takes place at the base of the leaf within the mine.

Puparium. ( Figs. 42–44 , 341 ) Straw-coloured, slightly shining, 3.8 mm long, with weak segmentation; surface quite smooth. Posterior spiracles set on stout conical protuberances that are entirely separate; black, glossy, fanshaped, with 15–16 yellow sessile bulbs arranged in irregular groups. Anal plate not protruding above the surface of the puparium viewed from the side and directed ventro-posteriorly.

Cephalopharyngeal skeleton. ( Fig. 342 ) Right mouthhook smaller than the left, both with ventral portion strongly and sharply abducted. Each mouthhook bears two accessory teeth with the upper much narrower than the lower. Intermediate sclerite long, narrow and straight, well sclerotized, 1.62× as long as height of the left mouthhook. The dorsal cornu bears a wide and long “open” window and narrow and short “closed” window located ventrally. The ventral cornu is more weakly sclerotized than the dorsal cornu. Indentation index 63.

Female head. ( Fig. 343 ) Black, with orbit slightly projecting above eye in profile, 2 orb s, 2 fr s and low, largerly semicircular lunule, reaching the level of the anterior fr s; pped of medium size, rounded. Gena is wider posteriorly, 0.18× as high as maximum height of eye. Vibrissa of medium size, directed dorso-anteriorly.

Male genitalia: ( Figs. 344–346 ) The original description ( Guglya 2013) of this species included deficient figures of male genitalia; more complete illustrations are provided here. The ejaculatory apodeme is large, oval, with paralleled margins, 0.6× as long as phallus; sperm sac relatively small, well sclerotized. The hypandrium is typical for the genus, strongly curved viewed from the side, with hypandrial apodeme wide and blunt viewed from below and hook-like viewed from the side.

Female genitalia. ( Figs. 347, 348 ) Blade of egg guide rather wide, 4× as long as maximum width, narrowed and rounded apically, with medial membrane bearing groups of scales along the inner lobe: small scales in basal half and larger scales in apical half. Outer marginal denticles acute, small but distinctly visible, directed apically. Proctiger 5× as long as maximum width. Blade of egg guide scarcely longer than proctiger. Spermathecae equal in size, brown, slightly wider than high and flattened basally. Internal duct invagination 0.8× as deep as height of spermatheca, widening apically. Basal collar consists of several long, narrow lobes, swirling around spermathecal duct. Spermathecal duct is very weakly sclerotized.

Distribution. Ukraine (Kharkiv and Kyiv Regions) ( Guglya 2013).

Figures

FIGURES 37–44. Figures 37–40: Ophiomyia maura (Meigen); 37: mine in Solidago virgaurea leaf; 38–39: posterior segments of puparium; 38: lateral view; 39: posterior view. 40: posterior spiracles (posterior view). Figures 41–44: O. versera Guglya; 41: mine with puparium (indicated by arrow) in Campanula rapunculoides leaf; 42: puparium viewed from the side; 43–44: posterior segments of puparium; 43: lateral view; 44: posterior view.

FIGURES 341–348. Ophiomyia versera Guglya; 341: posterior spiracle; 342: cephalopharyngeal skeleton; 343: female head viewed from the side; 344: hypandrium and postgonites viewed from below; 345: male genitalia proportionally; 346: hypandrium viewed from the side; 347 a–c: female genitalia proportionally; a: egg guide; b: proctiger; c: ventral receptacle. 348: spermatheca.