Strongylognathus afer

Sanetra, M. & Guesten, R., 2001, The socially parasitic ant genus Strongylognathus Mayr in North Africa (Insecta: Hymenoptera: Formicidae)., Zootaxa 20, pp. 1-20: 4-18

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9683

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scientific name

Strongylognathus afer
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[[ Strongylognathus afer  HNS  ]]

The holotype of Strongylognathus afer  HNS  from western Algeria (Dhaya), a dealate female deposited in MCSN, was compared with the other North African Strongylognathus  HNS  females available, all collected in Tunisia (Makthar, J. Gora’a, Nefza, Le Kef; see listing of material below). All these were morphologically virtually identical (see redescription below). The female sexuals however, were unknown for var. foreli  HNS  when Emery (1909) first described it. A comparison of the 8 worker samples now known from eastern Algeria and Tunisia provided convincing evidence for the conspecifity of all these populations, thus allowing the assignment of the Tunisian females to S. foreli  HNS  . In addition to the virtual identity of females from that region with the S. afer  HNS  holotype, investigation of workers from central Algeria and Morocco also revealed no differences of taxonomic significance from the Tunisian material. Consequently S. foreli Emery  HNS  , 1922 sinks into synonymy with S. afer Emery  HNS  , 1884.

Forel's material from his 1889 North Africa excursion (reported in detail in Forel 1890b) originally included Strongylognathus  HNS  workers from one Tunisian and two Algerian localities. Nevertheless, when Emery (1909) described var. foreli  HNS  based on material received from Forel, he mentioned only ,,Algeria“ as the type locality. Indeed, all 21 specimens in Emery's collection at MCSN bear the locality indication ”Duvivier”. Thus, the restricted type locality of S. foreli Emery  HNS  , 1922 is Bouchegouf (formerly Duvivier) in the Seybousse Valley, Wil. Annaba, Algeria.

Strongylognathus afer Emery  HNS  , 1884

Strongylognathus huberi huberi var. foreli Emery  HNS  , 1909 (name unavailable)

Strongylognathus huberi ssp. foreli Emery  HNS  , 1922: syn. nov.

Strongylognathus foreli Emery  HNS  : Bolton 1976

Collecting data: Tunisia - Gouv. Siliana, road Makthar-Tebessa, 6 rkm W Makthar, ca. 900m , X.1995, leg. A. Schulz & K. Vock: dealated [[queen]] crawling on the ground  ; Gouv. Siliana, Foret de Kesra, ca. 7 rkm N Kesra, ca. 1000m , 05.IV.1997, leg. M. Sanetra: numerous [[workers]] in a nest of Tetramorium semilaeve  HNS    ; Gouv. Béja, Mts de Teboursouk , Jebel Gora’a, ca. 800m , 04.IV.1997, leg. M. Sanetra: numerous [[workers]] and queen in a nest of T. semilaeve  HNS    ; Gouv. Béja, ca. 6 rkm S Nefza, Jebel Sidi Ahmed, ca. 300m , 11.IV. 1997, leg. M. Sanetra: numerous [[workers]] and queen in a nest of T. semilaeve  HNS    .

Other investigated material: Tunisia - 8 [[workers]], 4 [[queens]], 10 [[males]]: Le Kef [650m] , 1909/10, leg. Dr. Normand ( NHMB: 8 [[workers]], 4 [[queens]], 9 [[males]], MCSN: 1[[male]]; unpubl.)  ; 1 [[queen]], 1 [[male]]: Le Kef , „Dr. Santschi“[leg.?] ( NHMB; unpubl.)  ; 27 [[workers]]: Dir el Kef, 900m , 22.V.1913, leg. F. Santschi[?] ( NHMB: 26 [[workers]], IEGG: 1 [[worker]]; unpubl.)  ; 22 [[workers]]: Beja [250m] , IV.1889, leg. A. Forel ( MHNG: 11 [[workers]], NHMB: 4 [[workers]], IEGG: 1 [[worker]], ZMHB: 6 [[workers]]; Forel 1890a, 1890b)  ; Algeria - 12 [[workers]]: mt. near Souk-Ahras, close to summit, ca. 1500m[probably erroneous] , IV.1889, leg. A. Forel ( MHNG; Forel 1890a, 1890b)  ; 29 [[workers]]: Duvivier [now Bouchegouf, 300m] , IV1889, leg. A. Forel ( MHNG: 6 [[workers]], MCSN: 21 [[workers]] syntypes of Strongylognathus huberi ssp. foreli Emery  HNS  , IEGG: 2 [[workers]]; Forel 1890a, 1890b)  ; 13 [[workers]]: Massif de l´Ouarsenis, Teniet-el-Haad, 1500m , 10.V.1968, leg. H. Cagniant ( CXE; Cagniant 1970)  ; 1 [[queen]] holotype of Strongylognathus afer Emery  HNS  : Dhaya [1350m] , leg. Bedel ( MCSN; Emery 1884)  ; 1 [[worker]]: Saharian Atlas, Ain Aissa ravine, 1350m , 07.VI.1968, leg. H. Cagniant ( CHC; Cagniant 1970)  ; Morocco - 8 [[workers]]: High Atlas, Tizgui near Amezmiz, 1300m , 09.V 1983, leg. H. Cagniant & X. Espadaler ( CXE: 7 [[workers]], CHC: 1 [[worker]]; Cagniant 1997)  ; 2 [[workers]]: Rif mts., around Ras el Ma (near Chefchaouen) , 16.IV1984, leg. A. Tinaut ( CAT; unpubl.)  ; no locality label - 3 [[workers]]: probably Beja , IV. 1889, leg. A. Forel ( NHMB)  ; 1 [[worker]]: probably Duvivier , IV1889, leg. A. Forel ( IEGG) 

Further record (no material seen): Algeria - Massif de l´Ouarsenis, Jebel Berrouaghia, 850m , 27.III.1967, leg. H. Cagniant (Cagniant 1970) 

Female

Measurements and indices (n=9): ML 1.105±0.035(1.070-1.180)mm, HW 0.630 ±0.015(0.620-0.660)mm, HL 0.740±0.015(0.720-0.760)mm, SL 0.485±0.020(0.470- 0.530)mm, PW 0.250±0.015(0.230-0.280)mm, PPW 0.380±0.020(0.350-0.430)mm, CI 0.855±0.010(0.840-0.870), SI 0.660±0.020(0.625-0.695), PI 0.660±0.030(0.615-0.711)

Notably smaller than other species of the S. huberi  HNS  group execpt S. caeciliae Forel  HNS  , 1897 and S. minutus Radchenko  HNS  , 1991. Head considerably smaller than in worker, mesosoma only slightly longer; female/worker thorax volume ratio (see Stille 1996) 2.1-2.2 as measured for samples from Nefza, J. Gora’a and Le Kef. Head nearly as wide as long, widest at eye level or somewhat posteriorly (eyes excluded) but lateral margin only very slightly convex (Fig. 1). Occipital corners rather rounded and posterior margin weakly concave. Scape when directed backwards fails to reach occipital margin by about 1½ times its greatest width. Eyes situated medially on head sides, somewhat bulging (greatest diameter0.180-0.190mm). Head surface generally smooth and shining medially, almost without any sculpture in some specimens but shallow longitudinally striate over most of the surface in others. Lateral parts always longitudinally rugose with some anastomosing, posteriorly converging archedly to lateral ocelli. Pronotal corners rather angular, well visible from above. Propodeal teeth developed as short acute denticles, sometimes nearly absent. Mesoscutum with distinct longitudinal rugosity and weak microsculpture except for anterior central and posterior lateral portions, which are smooth and shiny, as are scutellum and metanotum except on their outermost parts (Fig. 2). Propodeum and often also lateral parts of mesosoma with well developed punctate-reticulate microsculpture and some rugae, especially around the pronotal corners. Petiolar nodes as in Fig. 3, surface largely covered with distinct microsculpture as on propodeum, weak to absent only around center of petiolus; rugosity scarce and indistinct if present at all. Body color dark blackish brown in fresh specimens, first gastral tergite and especially appendages lighter.

Variability: The nine specimens differ to some extent in sculpturing of the central head and lateral mesosoma surfaces, but are in general very similar to each other. The queen from Nefza, the only known female sexual from a lowland site, is considerably larger than the other specimens.

Worker

Measurements and indices (n=109): ML 0.950±0.050(0.830-1.050)mm, HW 0.695±0.035(0.590-0.770)mm, HL 0.795±0.035(0.670-0.860)mm, SL 0.530±0.025(0.460- 0.580)mm, PW 0.225±0.015(0.190-0.260)mm, PPW 0.295±0.020(0.260-0.330)mm, CI 0.875±0.015(0.840-0.940), SI 0.665±0.015(0.635-0.700), PI 0.765±0.025(0.705-0.815). Data apportioned to the separate samples are itemized in Table 1 (range not given).

Smaller than other species of the S. huberi  HNS  group except S. minutus  HNS  . Head scarcely longer than wide, widest at eye level (eyes excluded), in few specimens somewhat behind the eyes. Head sides variable, generally more convex than in females (Figs 4, 5). Occipital margin weakly concave. Convexity of head sides and concavity of occipital margin slightly more distinct in large examples, resulting in an allometric increase of CI with body size (see Tab. 1). Scape when directed backwards fails to reach occipital margin by about 1½ times its greatest diameter. Eyes situated medially on head sides, smaller than in females (greatest diameter 0.120-0.130mm) and bulging only very little, decidedly less so than e.g. in S. huberi  HNS  workers (compare to Fig. 20 in Sanetra et al. 1999). Head surface sculpture very restricted, lateral parts showing shallow longitudinal striation to a variable extent, posteriorly curving slightly towards center but then disappearing. Some individuals with few very weak short striae on center of head capsule. Pronotal corners can be rather angular, but smoothly rounded in other specimens. Propodeal teeth extremely variable from virtually absent to (rarely) rather distinct upright denticles. Lateral surfaces of mesosoma with variably developed irregular longitudinal rugosity, dorsal surface except propodeum completely smooth and shiny in most specimens but with punctate-reticulate microsculpture throughout and hints of longitudinal rugae in some. Petiolar nodes as in Fig. 6, surface appearing shiny but completely covered with shallow but dense microsculpture, with a pair of weak rugae laterally on postpetiolus or none at all. Body color varying from uniformly light yellowish-brown to yellowish-brown with darker centres of heads, sometimes body ochrish-brown and heads wholly dark brown.

Variability: The worker caste generally exhibits much more intra- and intercolonial variation in body size (see Tab. 1), head shape, surface sculpture and color than the investigated sexuals. At the lower altitude sites, specimens have been found to be comparatively large (Nefza, Béja) to medium-sized (Bouchegouf), lightly and uniformly colored, with the head sides relatively distinctly convex much as in S. destefanii  HNS  (Fig. 5, compare to Fig. 21 in Sanetra et al. 1999). Two samples from around Le Kef are much like the one from Béja. Workers taken on J. Gora’a are darker and distinctly smaller on average, but show extreme size variation. Their heads usually display an entirely dark brown coloration contrasting with other body parts, and - mainly in small individuals - less distinctly convex sides (Fig. 4), thus superficially resembling S. alpinus Wheeler  HNS  , 1909. Those from higher up in the Forêt de Kesra (which those from Souk-Ahras most strongly resemble) show heads not quite as dark and lighter on the sides than in the center, but color variability is very marked, some individuals being wholly yellowish-brown as at Nefza. However, Kesra workers are uniformly small in size. Unlike the other mountainous sites, the Algerian Teniet-el-Haad harbored the most lightly colored workers. Also, they are the smallest and very uniform. Some of the largest workers originate from the Moroccan High Atlas, having a slender appearance faintly recalling S. huberi  HNS  because of the narrowish mesosoma and in some instances a narrow head, but head shape as well as overall size are highly variable in this sample. Specimens from the Rif mountains show no noteworthy features. The single worker from the Saharian Atlas has the occipital corners more rounded than any other individual and shows some additional rugae on the postpetiolus but is otherwise much like specimens from northern Tunisia. As regards variability in surface sculpture, intranidal variation appears to be more distinct than that among colonies or populations. Variation in propodeal spine development can even be intraindividual, as the left and right side differ considerably in some specimens.

Male

Mesosoma length (n=10): ML 1.570±0.055(1.500-1.660)mm

Known only from two probably separate samples from Le Kef, Tunisia. Similar to female in size. Head slightly elongate with strong punctate-reticulate sculpture throughout. Mesosoma with fine longitudinal rugosity above a microsculpture mainly along sutures, otherwise smooth and shining. Propodeum and petiolar nodes bearing strong punctatereticulate sculpture, propodeal teeth similarly variable as in females. Male genitalia have not been examined as yet, owing to the very limited number of specimens and the undetermined taxonomic value of this character in the tribe Tetramoriini  HNS  .

Distribution and habitat specifications

The presented maps (Figs 7, 8) show the hitherto known distribution of Strongylognathus afer  HNS  in northern Africa. Although the information available is still rather scanty, the nine recorded localities (six of them newly published here) from eastern Algeria and Tunisia (Fig. 7) indicate that the species inhabits a wide variety of climatically and ecologically different sites. Only one locality in each country is situated on the northern slope of the coastal mountain range, where a true mediterranean-humid climate with a mean annual precipitation (m.a.p.) of at least 1000mm prevails. On the leeward side of the coastal mountain chain, Béja still exhibits a moderately humid climate (m.a.p. 630mm). Along the coastal ranges, natural climax vegetation would be olive-pistachio forest (Olea-Lentiscetum) on the north slope and mediterranean oak forest on the southern side, but today only remnants have survived in limited areas. Due to the intense exploitation as agricultural and grazing land, the search for Strongylognathus  HNS  in the coastal range proved challenging, and certainly the distribution of the species in that region is much more fragmented today than it was in former times. The colony discovered near Nefza had established its nest site at the margin of a small olive orchard, and in the surroundings, shrub vegetation mainly composed of Genista  was predominant.

In contrast to the above-mentioned sites, regions farther inland in Tunisia, such as the Medjerda valley and the so-called High Tell are to be included in the mediterranean-semiarid bioclimate zone roughly delimited by the 600mm isohyet ( Gießner 1984). The S. afer  HNS  records from the latter area (see Fig. 7) stem from heights of 800-1000m in prominent mountain ranges with somewhat higher humidity as well as lower winter temperatures. The single discovery in the corresponding part of Algeria (Forel 1890b) was made even higher up (though Forel's specification of 1500m seems incorrect as peaks around SoukAhras do not exceed 1250m). Mean annual precipitation near the mountainous collecting sites ranges from 510mm (Le Kef) to 730mm (Souk-Ahras). Aleppo pine associations combined with maquis containing holm oak and juniper would be naturally occurring there, rarely also true oak forests in the most humid situations ( Gießner 1984). However, the natural plant cover has been strongly degraded in many places by human influence. This applies in particular to the locality near Makthar, situated amidst grassy hillsides with limestone rocks heavily grazed by sheep and goats. In the nearby Forêt de Kesra, a colony of S. afer  HNS  was found on a southwest facing slope in a deforested area used by livestock. In the Mts de Téboursouk near the radio tower on J. Gora’a some grazing occurred also.

Two of the colonies reported by Cagniant (1970) were found in the central Algerian Massif de l'Ouarsenis at elevations above 800m. This part of the Atlas mountains is separated from the coastal range by the Chélif valley and immediately borders to the arid steppe zone in the south. Climatic conditions may be expected to resemble those in the Tunisian High Tell at the two localities close to Makthar (m.a.p. at Teniet-el-Haad 630mm). Cagniant (1970) reported a holm oak maquis with juniper at 850m and a shrubby clearing in cedar-dominated wood at 1500m. In the westernmost Algerian mountains, with the type locality of S. afer (Dhaya)  HNS  , generally drier conditions prevail and only the highest peaks may be comparable ecologically to the Massif de l'Ouarsenis. Surprisingly, another record has been obtained at this longitude even much farther to the south in the Saharian Atlas, beyond the very arid Hauts Plateaux. Particular environmental conditions evidently supported here, at least at the time of collecting, an open holm oak stand with juniper and olive trees in a steep-sided valley with permanent running water (Cagniant in litt.), even though at only slightly lower elevation at Aïn Sefra semi-desert conditions prevail (m.a.p. below 200mm).

According to Cagniant (in litt.), S. afer  HNS  was recorded in the Moroccan High Atlas at 1300m on a meadow with Asphodilus  sp. and Cistus  sp. along a creek, bordering an oak wood. No habitat information was availble for the locality in the Rif mountains.

Allozyme variation

In view of the largely unresolved systematics of Palaearctic taxa in the genus Tetramorium  HNS  and the difficulties of species delimitation through worker morphology, a preliminary survey of the Tunisian Tetramorium  HNS  fauna by allozyme electrophoresis was undertaken. Contrasting electromorphs at the Gpi, Idh and Pk loci yielded the recognition of two clearly distinct species or species groups (Tab. 2). Comparison with collection material identified these as T. semilaeve Andre  HNS  , 1883 and T. biskrense Forel  HNS  , 1904, corroborated also by electrophoretic data from elsewhere (Sanetra et al. 1999, Sanetra & Buschinger 2000, note that terminology of electromorphs is different in the latter paper). Interestingly, 18 of 21 colonies of T. semilaeve  HNS  were monomorphic at all loci studied ( T. semilaeve  HNS  (s.l.) from other parts of the Mediterranean region are more variable), while enzyme polymorphism was high in T. biskrense  HNS  . The two entities are also distinguishable by the morphology of sexuals and, though only subtly and often not convincingly, by different sculpture of the workers.

As elaborated in Sanetra et al. (1994, 1999), allozyme electrophoresis is not a valuable tool to distinguish species in the genus Strongylognathus  HNS  . The three investigated colonies of S. afer  HNS  did not differ from the usual pattern previously observed in other species of the S. huberi  HNS  group at the studied loci. At the Gpi locus, only one allele common to all investigated species was found (Tab. 2), whereas a second one was also present among six colonies of S. destefanii  HNS  from southern Italy studied in Sanetra et al. (1999).

Host species

The Tetramorium  HNS  colonies found infested with S. afer  HNS  contained no sexuals of the host species, as is to be expected for a dulotic social parasite (Buschinger et al. 1980). Electrophoretic investigation, however, identified Tetramorium semilaeve  HNS  (for latest attempts to define the species see Sanetra et al. 1999, and Cagniant 1997 for North Africa) as the sole host species in the three S. afer  HNS  colonies studied. In non-parasitized host colonies, single queens of T. semilaeve  HNS  were regularly discovered at the time of collection in early spring, hence monogyny can be assumed. A polygynous colony of T. maurum Santschi  HNS  , 1918 was found at Jebel Gora’a in proximity to a Strongylognathus  HNS  colony. This Tetramorium  HNS  species, described from Le Kef, exhibits somewhat enlarged petiolar nodes in females, whilst workers are at present morphologically indistinguishable from T. semilaeve  HNS  . T. maurum  HNS  is also electrophoretically identical to T. semilaeve  HNS  at the loci studied and may constitute a polygynous form of the latter species.

Tetramorium biskrense  HNS  apparently does not occur in the more northerly part of the range of S. afer  HNS  in Tunisia. However, near Makthar and in the Forêt de Kesra, it was found at the same sites where the slave-maker was detected. Nests of S. afer  HNS  and T. biskrense  HNS  were once found very close (1-2 metres) to one another. However, the suitability of the latter as host species has yet to be seen. The colony structure of T. biskrense  HNS  is at least facultatively polygynous, since five to ten queens (status determined by dissection) were sometimes observed in a nest.

The treatment of the Moroccan Tetramorium  HNS  fauna by Cagniant (1997) as well as results of an excursion to that country by us and others in 1995 revealed a greater species richness than in Tunisia, with taxa delimitations even more difficult to understand. Based on one or a few workers each investigated morphologically, hosts of the colonies of S. afer  HNS  found in Morocco, as well as those from Algeria (as already cited by Cagniant 1970), can apparently be assigned to a broadly conceived T. semilaeve  HNS  .

Slave raiding behavior

Three successful slave raids were observed in the laboratory, one by each of the three colonies of S. afer  HNS  collected in 1997, of which two were queen-right. These raids were directed towards queen-right host colonies of Tetramorium semilaeve  HNS  from Tunisia. On the other hand, in one experiment with the target colony consisting of T. caespitum (Linnaeus  HNS  , 1758) from Germany, also with its resident queen, the Strongylognathus  HNS  were not able to conquer, though scouting and initial recruitment were observed. Raids lasted for one or two days, usually starting at dusk. Two of the raids began when the ants managed to surmount the partitioning wall during the night despite measures against this, and entered the part of the arena containing the target colony. At the next morning the raid was already in progress. Nevertheless, all constituent elements of typical raiding-behavior (see Buschinger et al. 1980) could be documented for S. afer  HNS  .

During the characteristic recruitment on pheromone trails (Sanetra & Buschinger 1996), both Strongylognathus  HNS  and their host workers were engaged in trail running, but the parasites alone seemed responsible for chemical signalling. Tetramorium  HNS  host workers were much involved in combat activities, the intensity of which strongly differed between individual raids. In some cases, Strongylognathus  HNS  workers successfully pierced the head capsules of defending Tetramorium  HNS  with their saber-shaped mandibles. Often, though, the Strongylognathus  HNS  would behave passively towards foreign Tetramorium  HNS  , but almost always survived even vigorous attacks. After recruitment of more nestmates, the Strongylognathus  HNS  displayed peculiar behavioral patterns including quick running, threat with open mandibles and upright posture, obviously intimidating opponents in that way.

After having invaded the target colony, the Strongylognathus  HNS  carried away brood and even adults from the foreign nest. Interestingly, the transportation of adult host workers, a behavior exceptional among slave-making ants, was recorded in high frequency during all slave raids observed in S. afer  HNS  . Group recruitment occurred periodically in waves, alternating with periods of brood and adult transport. The duration of these periods varied from about one to three hours. The two experiments with queen-right Strongylognathus  HNS  colonies resulted in the death of the defeated host colony's queen. Unfortunately, it could not be definitely determined whether the queen was killed by the foreign Tetramorium  HNS  workers or by the slave-makers. In another instance the T. semilaeve  HNS  queen of the raided colony was adopted into the Strongylognathus  HNS  society at the end of a slave raid and survived there for over half a year until culture was discontinued. It should be noted, however, that this Strongylognathus  HNS  colony had been deprived of its resident queen, presumably during collecting.

Discussion

The treatment of Strongylognathus foreli  HNS  as a synonym of S. afer  HNS  is straightforward after the study of almost all existing material of the genus from North Africa. Already Emery (1909) himself had suggested in the first description that var. foreli  HNS  might constitute the worker caste of S. afer  HNS  , then went on to state: “In doubt I consider this variety as new”. Female sexuals, which are the most important morph for species distinction in the genus, from four sites in Tunisia and one in western Algeria (holotype of S. afer  HNS  ) were nearly identical. In addition, we were unable to decisively correlate any aspect of variation among worker samples with geographic, climatic or habitat variables. Thus, very little doubt remains that only one species of the S. huberi  HNS  group occurs in northern Africa.

It is now desirable to work out the taxonomic relationships of S. afer  HNS  to the similar species of neighboring regions, namely S. destefanii Emery  HNS  , 1915 from southern Italy, S. insularis Baroni Urbani  HNS  , 1968 from Malta and S. caeciliae Forel  HNS  , 1897 from the Iberian Peninsula (see distribution map, Fig. 8). Preliminary investigations showed that structural differences between these four species are weak to non-existent. The female holotype of S. caeciliae  HNS  is no more different from that of S. afer  HNS  than are Tunisian females. However, the worker caste of S. caeciliae  HNS  , though reported (de Haro & Collingwood 1977, Acosta & Martínez 1982, Espadaler 1997), remains undescribed. Its putative description by Baroni Urbani (1969) pertains in fact to S. huberi  HNS  as shown by Sanetra et al. (1999). The only obvious difference presently known to us between S. afer  HNS  and S. destefanii  HNS  concerns size, the latter species being somewhat larger on average, with currently no overlap in females. S. insularis  HNS  will doubtlessly prove synonymous to S. afer  HNS  and/or S. destefanii  HNS  . These observations corroborate the general finding that many nominal species of Strongylognathus  HNS  differ no more from each other than different populations or even colonies in most species groups of Tetramorium  HNS  (Bolton 1976). On the other hand, the exuberant degree of morphological variation in workers of the genus Strongylognathus  HNS  , as reported here for S. afer  HNS  , might well be prone to obscure existing differences between species. Hence, further studies on intraspecific variability in Strongylognathus  HNS  will be paramount to establish true species boundaries more convincingly.

One of the most striking features of S. afer  HNS  are the small female sexuals, which barely exceed workers in size. Some eastern Mediterranean and Asian species of the S. huberi  HNS  group have developed similarly minute females. In S. silvestrii Menozzi  HNS  , 1936, known from Greece and Turkey, heads of workers differ in shape from those in S. afer  HNS  , being narrower and more parallel-sided, and the sculpturing is more distinct on average. S. minutus Radchenko  HNS  , 1991 described from Turkmenistan and Uzbekistan is the only huberi-group species even smaller than S. afer  HNS  , and the head sides are reported to be not convex at all (Radchenko 1991). Nevertheless, the Asian fauna certainly needs further investigation to assess the significance of these characters.

According to present knowledge, S. afer  HNS  is the only social parasite of Tetramorium  HNS  in North Africa, in contrast to other countries along the coast of the Mediterranean Sea. The presence in the Maghreb of the inquilines Strongylognathus testaceus (Schenck  HNS  , 1852) and Anergates atratulus (Schenck  HNS  , 1852), widely distributed throughout Eurasia, seems unlikely because their most common host species, T. caespitum  HNS  , appears to be both local and rare. In Morocco, it has obviously been introduced to anthropogenic habitats along the coast and occurs naturally only on mountain tops of the Rif range (Cagniant 1997), while there are no reliable records from Algeria or Tunisia. Other dulotic Strongylognathus  HNS  species are most probably absent from North Africa as well. Thus S. afer  HNS  has been able to penetrate all ecologically suitable habitats without facing competition by other social parasites. Accordingly, the observed distribution pattern of S. afer  HNS  covers a relatively broad altitudinal range, at least from 300 to 1500m, while in other regions of the Mediterranean different slave-making Strongylognathus  HNS  species display preferences to certain types of habitat. For example, in southern Italy, S. destefanii  HNS  has primarily been recorded from coastal areas, while S. huberi  HNS  and S. alpinus  HNS  are confined to more mesic and higher mountainous biotopes, respectively (Sanetra et al. 1999). Only once, two of these species were found syntopically.

The area between the Mediterranean Sea and the Sahara desert in Tunisia and northeastern Algeria ranges from a mediterranean-humid to a mediterranean-arid bioclimate. S. afer  HNS  should be expected to occur more frequently along the coast, where climatic conditions are similar to those favored by its southern European relatives. However, a rather low proportion of records actually originates from that area (see Fig. 7), possibly because of the excessive degradation of habitats in coastal northern Africa. Our new discoveries of S. afer  HNS  in the environs of Makthar are probably located at the southern limit of its distribution, as the tectonically most important mountain ridge of the Tunisian Atlas, which stretches from south-west to north-east, forms a significant climatic transition zone. On its southeastern side, in the lee of the rainy winds, annual precipitation sharply decreases to below 300mm, leading to mediterranean-arid steppe vegetation ( Gießner 1964). The present collecting data suggest that these arid environments south of the climatic barrier are not tolerated by S. afer  HNS  . In addition to climatic conditions, the dominance of Tetramorium biskrense  HNS  over the host species T. semilaeve  HNS  in steppe habitats may also be a relevant factor. It is difficult to say if the apparent absence of S. afer  HNS  from less elevated parts of the Medjerda valley and the High Tell is due to the more arid conditions compared with the mountainous parts of that region, or to the excessive use as agricultural land since Punian times. Nonetheless, the change of the natural plant cover alone need not be devastating to S. afer  HNS  and a moderate grazing regime may even have advantageous impacts as the Tetramorium  HNS  hosts are open country inhabitants.

The distribution data hitherto available for S. afer  HNS  show a much lower density of records in Algeria and Morocco than in Tunisia (Fig. 8). Although the Algerian ant fauna has been extensively studied by Cagniant (1968, 1970), the data basis for this large country seems not yet sufficient to assess the abundance of S. afer  HNS  in the extensive central part of its range. Unfortunately, political circumstances in Algeria currently hamper further research here. The southernmost site in the Saharian Atlas, though geographically isolated, seems ecologically not untypical. Up to recent times there has been a complete lack of S. afer  HNS  records from myrmecologically rather well investigated Morocco, although its presence in the Rif mountains seemed highly likely, owing to records both from western Algeria and, of the very similar S. caeciliae  HNS  , from the Iberian Peninsula. This predictable occurrence in the Rif range has just recently been confirmed (Tinaut in litt.). Surprisingly, one additional record of S. afer  HNS  from far south in the High Atlas has become available (Cagniant 1997), but it remains the only colony found by H. Cagniant during his 17 years of collecting in Morocco (Cagniant in litt.). The apparent rarity of S. afer  HNS  in Morocco has also been corroborated by our excursion in 1995, when intensive searching in the High and Middle Atlas mountains failed to turn up Strongylognathus  HNS  . We cannot presently explain why S. afer  HNS  might be less common in Morocco than in Tunisia, since suitable habitats in a reasonable state of conservation, even with dense T. semilaeve  HNS  (s.l.) populations are frequent in many mountainous areas of Morocco. The systematics of the rather rich Moroccan Tetramorium  HNS  fauna is still barely understood (Cagniant 1997). Thus, a lower abundance of ordinary monogynous T. semilaeve  HNS  suitable as hosts might be one possible explanation, as we often observed polygynous colonies.

The host workers reported in this study and by Forel (1890b) belong to Tetramorium semilaeve  HNS  , in a broadly defined sense. Forel (1890b) additionally cited T. punicum (Smith  HNS  , 1861) as the host of S. afer  HNS  , but that name should be treated as a nomen dubium (Sanetra et al. 1999). A dark color form of T. semilaeve  HNS  is the best explanation for Forel's observation. T. maurum  HNS  (doubtfully distinct from T. semilaeve  HNS  , see results) and T. biskrense  HNS  were never positively identified as slave species of S. afer  HNS  , though the latter was frequently found nesting in close vicinity to Strongylognathus  HNS  colonies at the more southern localities. This apparent restriction to a single host species probably results from the common occurrence of multiple queens in T. maurum  HNS  and T. biskrense  HNS  , which might render colony foundation difficult for this slave-maker. However, the exact mode of colony founding remains unknown in Strongylognathus  HNS  . Founding queens in some dulotic ant species are adept in killing or driving off more than one host queen (e.g., Buschinger et al. 1980), but the use of polygynous slave species has not been recorded in Strongylognathus  HNS  so far. It seems nevertheless conceivable that polygynous Tetramorium  HNS  species or populations serve as additional hosts, whose workers are occasionally enslaved during raids but which may be unsuitable for colony foundation.

Most other nominal Tetramorium  HNS  taxa reported from Tunisia are probably based on local populations of T. semilaeve  HNS  ( jugurtha Menozzi  HNS  , 1934, atlante Cagniant  HNS  , 1970) or T. biskrense  HNS  ( kahenae Menozzi  HNS  , 1934, jarbas Cagniant  HNS  , 1970, both synonymized with T. biskrense  HNS  by Cagniant 1997). There is evidence that three more valid Tetramorium  HNS  species inhabit Tunisia, none of which, however, can be regarded as a potential host of S. afer  HNS  . Firstly, T. juba Collingwood  HNS  , 1985, a distinct species described from the Algerian Sahara, appears to occur in arid environments in southern Tunisia (sample from Nefta, Gouv. Tozeur, coll. A. Schulz; tentatively identified). The two other species, which clearly show Afrotropical affinities (see Bolton 1980), are T. sericeiventre Emery  HNS  , 1877, recorded from southern Tunisia by Santschi (1918), and T. exasperatum Emery  HNS  , 1891. The latter was found once during the 1997 excursion (7 rkm N Ghardimaou, ca. 600m, 12.IV.1997, leg. M. Sanetra), close to its type locality at Aïn Draham in the coastal range of northern Tunisia. It is related to the Afrotropical T. simillimum  HNS  (Smith, 1851) species group rather than to other Palaearctic Tetramorium  HNS  .

Observations by Forel (1890b) that the parasites are more frequent in the nests and larger than their hosts let him suggest dulotic life habits for S. afer  HNS  , which, however, had not been confirmed until now. The slave raiding behavior recorded in this study does not apparently differ from the usual pattern observed in other dulotic Strongylognathus  HNS  species of the S. huberi  HNS  group (Kutter 1920, 1923, Sanetra & Buschinger 1996, unpubl.). However, the peculiar trait of enslaving adult host workers, termed eudulosis by Kutter (1957), is markedly developed in S. afer  HNS  , while S. destefanii  HNS  exhibited this behavior only sporadically in laboratory experiments. Raiding adult workers is extremely uncommon in other dulotic ant genera (Buschinger et al. 1980), having but rarely been observed in Polyergus rufescens (Latreille  HNS  , 1798) by Le Moli et al. (1994). It is not yet understood by what means the Strongylognathus  HNS  actually overwhelm foreign host colonies. It seems that mechanisms other than mandible fight, such as ritualized dominance behavior and/or the use of chemical weapons, are valuable elements in finally defeating the raided colonies. Host queen incorporation by orphaned Strongylognathus  HNS  societies, also a surprising observation, has occasionally been recorded in other species in laboratory experiments (Sanetra & Buschinger1996).

The discovery of a sole dealate female in early October near Makthar indicates the season of swarming at that time, at least in the southern mountainous part of the range. It may occur somewhat earlier in the eumediterranean lowlands. In comparison to their Tetramorium  HNS  hosts and also S. testaceus  HNS  , development of sexuals in the Mediterranean species of the S. huberi  HNS  group is delayed, with a presumed swarming period not before the middle of September to the beginning of October (Sanetra unpubl.). Though much remains to be elucidated, with the findings added here to the previously scanty literature information, Strongylognathus afer  HNS  can now be regarded as one of the best investigated species of the genus.

NHMB

Switzerland, Basel, Naturhistorisches Museum

MCSN

Italy, Genova, Museo Civico di Storia Naturale "Giacomo Doria"

IEGG

Italy, Bologna, Universita di Bologna, Istituto di Entomologia "Guido Grandi"

MHNG

Switzerland, Geneva, Museum d'Histoire Naturelle

ZMHB

Germany, Berlin, Museum fuer Naturkunde der Humboldt-Universitaet

CXE

CXE

CHC

CHC

CAT

CAT