Ecclisomyia conspersa Banks 1907

Givens, Donald R., 2018, The Nearctic Ecclisomyia species (Trichoptera: Limnephilidae), Zootaxa 4413 (2), pp. 201-259 : 215-227

Ecclisomyia conspersa Banks 1907

Figs. 10a–10c ; 11a–11c ; 12; 13a, 13b; 14; 15a, 15b; 16a, 16b; 17a, 17b; 18a, 18b; 19a, 19b; 20a–20d; 21a–21c; 38; 41; 42; 43b.

Ecclisomyia conspersa Banks 1907 , 123, pl. IX, fig. 14, Holotype male: Olympia, WA. Ross (1950, 425, figs. 16a, b, male genitalia, distribution); Anderson (1976, distribution, bionomics); Denning (1951, 162, distribution); Schmid (1955, 59, figs. 33 and 34, descriptions of male genitalia, female genitalia); Flint (1960, 24, figs. 5a–d, description of larva); Nimmo (1971, 62, figs. 194–199, 599, descriptions of male genitalia, female genitalia, bionomics, distribution); Baumann & Unzicker (1981, 27, distribution); Herrmann et al. (1986, 440, distribution); Irons (1988, 1260, biology, distribution); Schmid (1998, 88, 89, figs. 301–307, descriptions of male and female genitalia); Wiggins (1996, 304, fig. 20.14, description of larva); Givens (2014, 160, 170, distribution).

Male diagnosis. The single black stout basal spine ( Fig. 10a ) arising from each inferior appendage separates E. conspersa from the other three Nearctic species. The inferior appendages of E. bilera lack spines; those of E. maculosa and E. simulata each have 2 long black stout basal spines arising from the mesobasal area ( Figs. 22b , 23b , 30b , 31b ). The parameres of E. conspersa are narrow, rounded, and nearly uniform in diameter ( Fig. 11a ); those of E. bilera are narrow caudally with distal ends angled dorsad ( Figs. 1a , 2a ); parameres of E. maculosa and E. simulata are fused and with the distal ends incised ( Figs. 22b , 30b ).

Male description. See Banks (1907); Schmid (1955); Schmid (1998); and Nimmo (1971).

Female diagnosis. The distal margin of tergum X ( Figs. 12 , 13a ) has a shallow mesal notch, unlike the U to V incisions in terga X of E. bilera , E. maculosa , and E. simulata . The external part of gonopod VIII is narrower than in E. maculosa and E. simulata . The external part of gonopod IX is more recessed than in E. maculosa and E. simulata . The spermathecal sclerite is narrow and elongate ( Figs. 12 , 13a ), not short and quadrate as in E. maculosa and E. simulata ( Figs. 24c , 25b, 25c , 32a, 32b ).

Female description. See Schmid (1955, 1998); Nimmo (1971). A supplementary description follows: Length 15–19 mm (N = 12). Viewed from ventral aspect external part of gonopod VIII short, narrow, wrinkled mesally, with lateral margins sinuate, caudal margin convex. External gonopod of segment IX short, nearly as wide as long; caudal margin with wide V-shaped indentation mesally. Spermathecal sclerite narrow and elongate ( Figs. 12 , 13a ). Tergum IX fused with tergum X; tergum X with shallow apicomesal notch ( Figs. 12 , 13a ).

Pupal diagnosis. Ecclisomyia conspersa pupae may be separated from the other three Nearctic Ecclisomyia species by the presence of lateral line gills on abdominal segments IIp–IVa ( Fig. 42 ), lacking in E. bilera , E. maculosa , and E. simulata ( Fig. 42 ). Each apical process of E. conspersa has 3 long, black stout setae at the apex ( Figs. 15a, 15b , 17a, 17b ), whereas those of E. bilera , E. maculosa , and E. simulata each have 2 long black stout setae at the apex ( Figs. 5a , 6a , 7a, 26a, 26b , 27a , 28a, 33a, 33b , 34a , 35a). The apical process lobes of E. conspersa pupae are straight ( Fig. 15a ), not mesally convergent as in the other Ecclisomyia species, however the apical processes of males of E. simulata may sometimes be directed straight caudad, not mesally convergent. The pupal antennae of the E. conspersa female extend to or slightly posterior to the anterior margin of abdominal segment VI, and may extend to the anterior margin of abdominal segment VII. In the other three species the female pupal antennae extend only to the posterior margin of abdominal segment V.

Pupal description. Length 11–15 mm (N = 10). Head: Black to dark brown. Male antennae extending to posterior margin of abdominal segment VIII; may extend caudad of anterior margins of apical processes. Antennae of female extending to anterior margin of abdominal segment VII; may extend to posterior margin of segment VII. Labrum light brown; posterior margin convex, rounded anteriorly, with 10 long black setae on dorsal surface, apices of setae hooked ( Fig 20a ); anterior margin with 2 pairs of short, translucent setae, each pair lateral of meson. Mandibles reddish brown each with broad base, narrowing apically, forming thin flattened tooth, mesal margin minutely serrated (serrations difficult to discern with light microscope); ventral base with 2 long black setae ( Fig. 20b ).

Thorax: Pro-, meso-, and metanota dark brown. Pro-, meso-, and metathoracic legs brown to yellow-brown. In mature pupae pro-, meso-, and metatibiae each with both single and paired short black spines; tarsal segments of all legs with both single and paired short black spines. Immature pupae without spines. Procoxae of both mature and immature pupae each with 1 long black setae near lateral margin and 4–10 shorter black setae on ventral surface. Mesocoxae each with 1–3 black seta on ventral surface. Profemora each with single short black spine on ventral surface near distal margin. Mesotarsal segments 1–4 each with pair of lateral fringes of long reddish to white hairs directed posterad.

Abdomen: Lateral fringe on each side consisting of long, fine, hair-like black setae, directed dorsad; line of fringe arching ventrad to segment VIII. Abdominal gills present; dorsal, lateral, and ventral gills each singlefilament; present dorsoposteriorly and ventroposteriorly on segments II–VII; present dorsoanteriorly and ventroanteriorly on segments III–VII; lateral line gills present anteriorly on segments III and IV, posteriorly on segments II and III ( Fig. 42 ). In more mature pupae dorsal and ventral gills on segments IIp–Va subdorsal and subventral, respectively; gills on segments Vp–VIIIp dorsal and ventral. Two lateral-line gills on each side of the abdomen positioned such that one gill is dorsal of lateral line and one ventral of lateral line ( Fig. 42 ). Dorsal process of segment I rugulose, with 2 black sa 2 setae each positioned lateral of meson; posterior margin sinuate, lightly sclerotized, brown, forming pair of denticulate submesal lobes, each lobe with 6–8 small, short spicules ( Figs. 20d ); shape of dorsal process varies with maturity of pupa. Hook plates on abdominal segments III–VII ( Figs. 20c , 38 , 41 ). Pairs of hook plates present anteriorly on segments III–VII, posteriorly on segment V. Hook plates IIIa each with 3 to 4 hooks; IVa with 2–5; Va with 3–5; Vp with 10–22; VIa with 3–5; VIIa with 2–5. Hook plates rugose, hooks reddish-brown, directed posterad on hook plates IIIa–VIIa; directed anterad on elliptical hook plate Vp. Dorsal abdominal segments each with 1 pair of long black sa 2 setae on each of segments I–V; segment V may have 2 pairs of short black setae; segment VI with 1 pair of sa 1 setae, short and difficult to discern and with 1 pair of short black sa 2 setae, and an additional 2–4 pairs of long black setae lateral of meson, sa 2 setae longer than lateral setae. Segment VII with 3–4 pairs of black setae lateral of meson near posterior margin of segment, sa 2 setae longer than lateral setae. Abdominal segment VIII with 3–7 setae lateral of meson. Venter of abdominal segments II–VIII each with pair of short black sa 2 setae, and segments III–VII each with pair of short black sa 3 setae. Segment VIII with additional 2–3 short black to translucent setae lateral of meson, near posterior margin of segment ( Fig. 20c ). Posterior area of segments VIII and IX with secondary reddish hl setae. Distal area formed into 2 caudal lobes (anal processes), caudal area of each lobe appearing punctate due to numerous reddish spicules, punctation difficult to discern with light microscope. ( Figs. 15a , 17a ). Caudal lobes each with 3 long, black, stout setae on distal margin of each lobe; each lobe with 2 shorter black, stout setae along mesal surface ( Figs. 15a , 16a, 17a, 18a). Dorsal surface of apical processes each with transverse row of 6–10 fine black setae ( Figs. 15a , 17a ). Lobes of apical processes extending caudad, not curving mesad; lateral margins rugose; ventral surfaces with numerous long black setae ( Figs. 15b , 16b, 17b, 18b).

Pupal case. Length 14–16 mm (N = 5), width at anterior end 2.5–4.0 mm; width at posterior end 2.0–3.5 mm (N = 9). Anterior end of pupal case 0.5 mm wider than posterior end ( Fig. 43b ). Case compact, constructed of large pebbles, interstitial spaces between larger pebbles filled with smaller grains of sand. Ends of case less compact, with small open areas to permit flow of water; an inner silk lining enclosing pupae.

Larval diagnosis. The E. conspersa larva may be separated from the other three Nearctic species by the presence of lateral line gills on abdominal segments II–IV ( Fig. 42 ), lacking in E. bilera , E. maculosa , and E. simulata ( Fig. 42 ). The E. conspersa larva has anterodorsal and anteroventral gills on abdominal segment VII, lacking on the E. maculosa larva. Ecclisomyia conspersa is without dorsoanterior gills on abdominal segment II, but E. simulata larva has them ( Fig. 42 ). The larva of E. conspersa is larger (9–16 mm) than larvae of the other three species of Nearctic Ecclisomyia (9–12 mm) long.

Larval description. See Flint (1960, 240); Wiggins (1996, 304, Fig. 20.14). A supplementary description follows: Length (mature instar) 12–16 mm (N = 8). Mature E. conspersa larva from Colorado 9–10.5 mm (N = 2). Head: Dark brown to reddish brown; area about coronal suture and lower posterior frontoclypeal suture yellowish; muscle scars on frontoclypeus and lateral of coronal suture indistinct; muscle scars numerous, distinct posterior and ventral of eyes on parietals. Parietals reddish brown, glabrous. Mandibles black, each with 4 small, blunt apical teeth ( Fig. 19a ). In mature larva, mesal tooth of each mandible sometimes so worn that only 3 blunt apical teeth apparent; mesal margins of mandibles each with tuft of translucent feather-like setae ( Fig. 19a ); posterolateral area of each mandible with 1–2 short translucent setae; ventral apodome triangular; 1.23–5.00 X longer than ventral ecdysial line (N = 6) ( Fig. 19b ); ventral apodome length to length of ventral ecdysial line increasing with maturity of instar. Primary setae in positions 7, and 9–17 ( Fig. 19a ); primary setae in position 7 short, reddish; primary setae in position 9 long, black; primary setae in primary position10 short, translucent; primary setae in position 11 fine, short, translucent or black; primary setae in position 12 short; primary setae in position 13 short, translucent, hard to discern; primary setae in positions 14, and 15 long, black; primary setae in position 16 short, translucent, hard to discern; and primary setae in position 17 long and black; primary setae in positions 11, 13, 16, and 18 sometimes absent; venter of head with primary setae in positions 8 and 18 ( Fig. 19b ); setae short, translucent, difficult to discern. Frontoclypeus reddish brown, wide anteriorly, narrowed posteriorly, triangular, glabrous except for primary setae in positons 1–6 ( Fig. 19a ); primary setae in positon 1 short, translucent; primary setae in positions 2 and 3 long, reddish; primary setae in position 4 translucent, appressed, long; primary setae in position 5 long, black; primary setae in position 6 short, translucent. Labrum reddish brown to dark brown; primary setae in positions 1–6 ( Fig. 19a ); primary setae in positions 1 and 3 short, translucent, difficult to discern; primary setae in position 2 and 4–6 short, translucent; light, short translucent setae present at anterolateral corners of labrum, forming sparse setal tuft. Cardo sclerites rectangular. Submental sclerite with 2 short black setae set lateral of meson; setal sockets prominent.

Thorax: Pro- and mesonota yellowish brown, nota with mesal yellow strip; metanota sclerites brown. Posterior margin areas of pro- and mesonota black, sclerotized; black sclerotized area of mesonotum curving halfway anterad along lateral margins. Mesonotal lateral processes triangular, black. Pro- and mesonota with mesal yellow stripe. Muscle scars indistinct on mesonotum lateral of yellow striping. Pronota with 8–12 long, black slender setae set equidistant along margin; short translucent setae set between these setae along anterior margin. Pronotal halves each with 4–7 long, black setae posterior to mid-dorsal transverse groove. Mesonotum with black setae in 3 distinct groups roughly corresponding to sa 1, sa 2, and sa 3 positions. Metanotal sa 1 sclerites triangular, each with 4–6 black ls setae. Metanota sa 2 sclerites each with 9–12 black ls setae. Metanotal sa 3 sclerites parenthesis-shaped, each with 10–15 black ls setae on anterior third of sclerite. Prosternal horn short, lightly sclerotized. Prosternal sclerite present, lightly sclerotized. Legs reddish brown. Foretrochantins dark brown; forming tent-like structures over procoxae. Procoxae with primary setae in positions 1 and 2. Ventral edge of each profemur with 2 long stout, reddish sl setae and short, translucent to reddish sl setae along ventral margin ( Fig. 21a ). Ventral edge of each mesofemur with translucent to reddish short sl setae along ventral margin; these sl setae sometimes also occurring along ventral margin of metafemora, but indistinct if present. Pro-and mesotibiae each with short translucent sl setae along distal ventral margin; these sl setae lacking along ventral margin of each metatibia. Protibiae and protarsi each with primary black seta in position 3 ( Figs. 21a, 21b ). Ventral margins of pro-, meso-, and metatrochanters with distal trochanteral brush, setae feather-like, translucent, extending to proximal ventral surface of each femur ( Figs. 21a–21c ). Trochanters of all legs with primary setae in positions 2, 3, and 5; primary setae in position 5 may be absent ( Figs. 21b, 21c ). Meso- and metacoxae each with primary seta in position 2. Coxal sclerites with 6–17 black ls setae on dorsal margin. Profemora with primary setae in position 2. Mesofemora with primary setae in positions 2 and 3. Metafemora with primary setae in positions 2, 3, and 4 ( Fig. 21c ). Pro- and mesotrochanters each with single short clear sl seta adjacent to primary seta in position 3. Mesofemora each with 2 long black ls setae on ventral surface.

Abdomen: Abdominal segment I with 11–17 black ls and ss setae on and lateral of dorsal hump. Dorsal hump generally without sclerites. Lateral humps without sclerites, each with 6 or 7 short black setae dorsally, sometimes with sclerotized bases. Ventral base of each lateral hump with 1 or 2 black ls setae. Area ventral of each lateral hump with 5 short black setae. Venter of abdominal segment I with 17–27 black short setae with prominent setal sockets; posterior setae with sclerotized bases. Venter of abdominal segment I sometimes with pair of sclerites or small sclerotized areas lateral of longitudinal axis, each with 2–4 black ls setae, bases of setae sclerotized. Abdominal gills present; dorsal and ventral abdominal gills each single-filament; present dorsoposteriorly and ventroposteriorly on segments II–VII; present dorsoanteriorly and ventroanteriorly on abdominal segments III–VII; 2 lateral-line single filament gills on each side of abdomen present anteriorly on segments III and IV and posteriorly on abdominal segments II and III, positioned such that one gill dorsal of lateral line and one gill ventral of lateral line ( Fig. 42 ). Early instar larvae 2.5 mm long sometimes lacking gills; instar larva 4.5–5.0 mm in length with only dorsoposterior and ventroposterior gills on abdominal segments II–VII and with no anterior gills; some larva 5.0 mm long with lateral-line gills and full complement of dorsal and ventral gills. Dorsum of abdomen with short, black sa 1 setae on abdominal segment I; dorsum of abdominal segments II–VII with short, black sa 2 setae; dorsal abdominal segments VI and VII sometimes with sa 2 setae long and fine, not short and fine, and sometimes each adjacent to l short, black setae. Segment VIII sometimes with 2 to 3 black to reddish setae adjacent to each sa 2 seta. Venter of abdominal segment II with pair of short black sa 1 setae lateral of meson; venter of abdominal segment IX with 2 black ls setae and 2 ss black setae, mesal pair shorter. Abdominal tergum IX with elliptical sclerite bearing 4–6 long, black, stout setae spaced equidistant from each other along posterior margin; surface of sclerite with 5–7 shorter, black to translucent setae. Lateral fringe of hl setae on each side extending from anterior of (sometimes posterior of) abdominal segment III to anterior of segment VIII. Chloride epithelia present on venters of abdominal segments III–VII; chloride epithelia sometimes visible only on segments IV–VII. Anal prolegs brown; lateral sclerites each with basal tuft of 2 long, black to reddish-black stout setae and 2 fine, short, black to reddish-black setae; dorsoposterior margin with 3–4 black ls setae; 7–11 short, black setae on dorsal surface. Sole plates each with single black seta near distal-ventral margin. Caudal margin of each anal hook with 3–4 short black setae and with small dorsal denticule. Caudal lobes glabrous, except for minute sl tan to reddish spicules on either side of anal opening.

Distribution. Of the Nearctic Ecclisomyia species, E. conspersa has the widest geographical distribution ( Denning 1951, Ross 1950). Material examined during this study included Alaska, Alberta, British Columbia, California, Colorado, Idaho, Montana, Oregon, Washington, and Yukon Territories. This species has also been reported from New Mexico ( Nimmo 1971) and Wyoming ( Swegman & Ferrington 1980). Ecclisomyia conspersa has been recorded from British Columbia ( Ross & Spencer 1952; Nimmo 1971) and the Yukon Territories ( Nimmo 1971). Ross (1950) listed E. conspersa from Alberta. Rasmussen and Morse (2014) listed E. conspersa from Utah and the Yukon Territories. Givens (2014) recorded E. conspersa as occurring in Lassen Volcanic National Park, California.

Bionomics. See Nimmo (1971), Irons (1988), and Wiggins (1996). I have collected Ecclisomyia conspersa from an altitudinal range of 318–1966 m. Nimmo (1971) indicated an altitude range of 792–2225 m. Emergence begins in mid-April and extends into mid-August. Ecclisomyia conspersa occupies a wider range of lotic habitats than the other three Nearctic Ecclisomyia species. My collections indicate streams with a width from 0.61–9.10 m, and a depth from 0.13–0.91 m. Streams usually were characterized by a substrate of cobble, gravel, and sand, frequently with boulders, and mixed with woody debris. Ecclisomyia conspersa has been reported to be sympatric with E. bilera and E. simulata ( Givens 2014) .

Material Examined. CANADA; Alberta, Maligne Rd. at Jct. of creek from Mt. Tehara, Jasper Nat’l. Park, 28-vii-1965 1 M (APN) (SMEC); Rapids Creek, Three Sisters Camp nr. Camore, 17-v-1966, 1 M (APN) (SMEC); Rapids Creek, 3 km NE of Rapids Gap, (T. C. Hwy.), 6 M, 1 F (APN) (SMEC); Rapids Creek, S of T. C. Hwy., 16- vii-1967, 1 M (APN) (SMEC); Miette River, 4.8 km W of Jasper (Hwy. 16), Jasper Nat’l. Park, 05-vii-1966, 6 M (APN) (SMEC); Miette River, 1.6 km S of Jasper, at bridge, at Jct. with Athabasca River, (nr. Hwy. 93), Jasper Nat’l. Park, 06-vii-1966, 1 F (APN) (SMEC); creek, 25.7 km S of Jasper and 1.6 km S of Hardesty Creek, Jasper Nat’l. Park, 06-vii-1966, 1 M (APN) (SMEC); Red Earth Creek, (T. C. Hwy.), Banff Nat’l. Park, 20-vii-1966, 2 M (APN) (SMEC); 16-vii-1967, 6 M, 8 F (APN) (SMEC); 21-vii-1968, 5 M (APN) (SMEC); creek, ca. 4.8 km down Rd. from Edith Cavrel Hostel, Jasper Nat’l. Park, 25-vii-1966, 1 M (APN) (SMEC); Wolverine Creek, ca. 12.8 km W of Banff, (T. C. Hwy.), (Trans-Canada Bridge), 26-vii-1966, 1 M (APN) (SMEC); Whitehorse Creek, at Mountain Park Rd., S of Cadomin, 21-v-1967, 25 M, 15 F, 7 M P, 4 F P (APN) (SMEC); Whirlpool River at bridge (Hwy. 93a), Jasper Nat’l. Park, 22-v-1967, 3 M (APN) (SMEC); Bath Creek, K. H. Pass (T. C. Hwy.), Banff Nat’l. Park, 23-v-1967, 1 M (APN) (SMEC); Whitehorse Creek, Cadomin, N52.983 W119.35, 07-viii-2007, 2 M, 3 F (M. Djevnes) (SMEC); Portal Creek, (Hwy. 93), Jasper Nat’l. Park, 2 M (APN) (SMEC); Fiddle River, (Hwy. 16), Jasper Nat’l. Park, 22-v-1967, 2 M (APN) (SMEC); 16-vi-1967, 1 M (APN) (SMEC); 09-viii-1967, 1 F (APN) (SMEC); 15-vii-1968, 1M (APN) (SMEC); 22-vii-1968, 2 M (APN) (SMEC); Snaring River, N of Jasper (Hwy. 16) Jasper Nat’l. Park, 24-vi-1966, 1 M (APN) (SMEC); 22-v-1967, 2 M (APN) (SMEC); 09-viii-1967, 1 F (APN) (SMEC); creek, 3.2–4.8 km N of Athabasca Falls, (Hwy. 93), Jasper Nat’l. Park, 26-v-1967, 1 M (APN) (SMEC); 15-vi-1967, 1 M (APN) (SMEC); Pass Creek, (Hwy. 5), Waterton Nat’l. Park, 14-vi-1967, 2 F (APN) (SMEC); Ranger Creek, (Hwy. 93), Jasper Nat’l. Park, 15-vi-1967, 1 F (APN) (SMEC); Mosquito Creek, (Hwy. 93), Banff Nat’l. Park, 15-vi-1967, 1 M (APN) (SMEC); Rampart Creek, (Hwy. 93), Banff Nat’l. Park, 22-v-1967, 2 M (APN) (SMEC); 15-vi-1967, 1 F (APN) (SMEC); Bauerman Creek, 1.6 km W Twin Lakes Cabin, Waterton Nat’l. Park, 05-vii-1967, 1 M (D.J. and M.A. Larson) (SMEC); Red Earth Creek, (T. C. Hwy.), Banff Nat’l. Park, 16-vii-1967, 6 M, 8 F (APN) (SMEC); East face of Tonquin valley, Jasper Nat’l. Park, 05-viii-1967, 1 M (APN) (SMEC); South Creek, Forest Trail Rd., S of N Sark River, 12-viii-1968, 2 M, 1 F (APN) (SMEC). British Columbia, Kootenay River, (T. C. Hwy.), Campfield, 23-v-1967 1 M (APN) (SMEC); River, Kootenay Hwy., Yoho Nat’l. Park, (T. C. Hwy.), V-23-1967, 3 M (APN) (SMEC); Kootenay Hwy., Yoho Nat’l. Park, 15-vi-1967, 1 F (APN) (SMEC); Kootenay R., K. crossing at bridge, Kootenay Nat’l. Park, 16-vi-1967, 1 M (APN) (SMEC); tributary to Summit Creek, (Hwy. 3), V-18-1968, 1 F (APN) (SMEC); Albreda (Hwy. 5), first crossing S of Valemount, 26-vi-1981, 1 F (APN) (SMEC); Lake, R-14, Mt. Revelstoke Nat’l. Park, 08-viii-1982, 2 M, 1 F (D.B.D.) (SMEC); Silverhope Creek, 15 km S of Hope, (Silverhope Creek Rd.), N49.266 W121.383, 17-vi-1987, 3 M, 3 F (GWC) (RWWPC). Yukon Territory, N. Fork Pass, Demster Hwy., km 78.5, Ogilive Mountains, 09-vi-1981, 1 F (D.C. Currie) (SMEC); creek, Demster Hwy., km 66.6, 15-vi-1981, 1 M (D.C. Currie) (SMEC); 01-vii-1981, 1 F (D.C. Currie) (SMEC); Kluante Lake, 06-vi-1982, 1 M (SMEC); Alaska: Fairbanks Borough, Auke Lake, Fairbanks, 18-vi- 2011, 2 M P, 3 F P (M. Fipli) (RWWPC); Haines Borough, 13.7 km N Haines, N59.271 W135.646, 19-vii-2001, 4 M, 3 F (C. Ferris) (CSUC). Matanuska-Susitna Borough, Moose Creek, Moose Creek Cmpgd. (Hwy. 1, Glenn Highway), 27-iv-2016, 7 M, 8 F (CJV) (CSUC). Sika Borough, Sawmill Creek, nr. Sitka, 20-iv-2004, 1 M, (J. Ptacek) (CSUC). Municipality of Anchorage, small stream ca. 4.8 km W of Girdwood, (Hwy. 1, Stewart Highway), ca. mile marker 93 (km 149.6), 04-vi-2006, 2 M P, 1 F P, 6 P, 1 PP (DER) (DERPC). E. Fork Steward Creek, Steward Hwy. N60.73174 W149.34, 24-iv-2016, 2 M (CJV) (CSUC); Ship Cr., Arctic Valley Rd., N61.16646 W149.65, 27-iv-2016, 6 M, 4 F, (CJV) (CSUC); Ship Creek, pedestrian bridge nr. golf course, N61.1235 W149.819, 1 M (CJV) (CSUC); N. Fork Campbell Creek, Kalmia Dr., N61.16646 W149.686, 1 M (CJV) (CSUC). CALIFORNIA: Butte Co., Colby Creek, (Humboldt Rd.) N40.11138 W121.49526, 25-v-2014, 1 M (BCK and CJV) (CSUC). Humboldt Co., Jacoby Creek, 26-x-1979, 1 L, (D. Lacuck) (CASC); Strong’s Creek, 10-x-1996, 1 L (JJL) (JLLPC); Freshwater Creek, 06-xi-2001, 1 L, (JJL) (JLLPC); Upper Mill Creek, Hoopa Valley, 15-iii-2015, 2 L (JJL) (JLLPC). Plumas Co., Upper Rice Creek, 23.2 km NW Chester (Stump Ranch Rd.), Upper Creek, Cmpgd., Lassen Nat’l. Forest, 29-vii-2012, 1 F P (DRG) (DRGPC). Shasta Co., Hat Creek, at Old Station, 07-xi-1981, 7 L, (D. Lacuck) (CASC); West Fork Hat Creek, 15 km S of NW entrance to Lassen Volcanic Nat'l. Park (Lassen Park Rd.), 19-vii-2011, 6 M, 8 F, 3 M P, 4 F P, 2 P, 5 PP (DRG) (CSUC); 20-vii-2011, 1 F (DRG) (CSUC); 22-vii-2011, 1 M (DRG) (CSUC); 26-vii-2011, 4 M, 3 F (DRG) (CSUC); Manzanita Creek, 2.25 km S of N entrance to Lassen Volcanic Nat'l. Park (Lassen Park Rd.), 24-vii-2011, 1 F (DRG) (CSUC); Lost Creek, 11.9 km S of NW entrance to Lassen Volcanic Nat'l. Park (Lassen Park Rd.), 20-vii-2011, 1 F (DRG) (CSUC); King's Creek, King's Creek Picnic Site 28 km S of NW entrance to Lassen Volcanic Nat'l. Park (Lassen Park Rd.), 13-viii-2012, 4 L (DRG) (CSUC). Siskiyou Co., Sugar Creek, tributary to Scott River (Siskiyou Resource Conservation District), 16-x-2006, 7 L (RWW) (RWWPC). Tehama Co., small creek, 0.3 km N Mill Creek (Hwy. 172) Lassen Nat’l. Forest, 01-vii-2011, 1 F (DRG) (CSUC). COLORADO: Alamosa Co., stream, Zapada Falls, 14-vii-1989, 1 F (J. Welch) (CSUC). Clear Creek Co., Dry Gulch (N I-70, exit 216), N39.7034 W105.9031, 12- vii-2014, 1 F (BDH) (CSUC); Hoop Creek, 8.8 km W of Empire (off Hwy. 40), N39.7789 W105.78353, 28-i-2008, 2 L (BDH) (CSUC). Hinsdale Co., El Paso Creek, base of Uncompahgre Park, 12-viii-1992, 3 M (S. Fitzgerald) (CSUC). Larimer Co., Alpine brook, 2.4 km NW of Chasm Lake, Rocky Mt. Nat'l. Park, 19-vii-2009, 1 M (BDH) (CSUC). Park Co., springs along South Mosquito Creek, upstream from London Mine, 28-V-1980, 4 M P, 1 F P, 11 P (DER) (DERPC); pool on N side road between Mosquito Creek and Louden Butte mine, 10-vi-1980, 2 L (DER) (DERPC). Saguache Co., Jones Creek, 1 km NE Little Sand Creek, and Sand Creek confluence, Great Sand Dunes Preserve, N37.89362 W105.49791, 08-ix-2004, 1 L (REZ) (CSUC); Little Sand Creek, ca. 2.6 km upstream from Sand Creek, Great Sand Dunes Preserve, 23-vi-2004 1 F (REZ) (CSUC); Lower Sand Creek, ca. 0.2 km downstream from Lower Sand Creek Lake, Great Sand Dunes Preserve, N37.93015 W105.52879, 21-vi-2004, 26 M (REZ and J.F. Bruce) (CSUC); Sand Creek, ca. 2.8 km SE of Lower Sand Creek Lake, Great Sand Dunes Preserve, N37.92606'N W105.51265, 21-vi-2004, 3 M (REZ) (CSUC); Sand Creek 4.3 km NW of Music Pass trailhead, Great Sand Dunes Preserve, N37.93598 W105.52446, 21-vi-2005, 3 M (REZ and J.F. Bruce) (CSUC); Sand Creek, ca. 0.4 km below Upper Sand Creek Lake, Great Sand Dunes Preserve, N37.94366 105.53512, 08-vii- 2005, 2 M (REZ) (CSUC); Medano Creek, at Trail Crossing ca 2.8 km downstream from Lake Medano, Great Sand Dunes Preserve, N37.86144 W105.45815, 23-vi-2005, 2 M, 1 F (REZ and J.F. Bruce) (CSUC); Medano Creek, up from confluence with tributary that drains lower Medano Lake, Great Sand Dunes Preserve, N37.86438 W105.47540, 23-vi-2005, 1 M (REZ and J.F. Bruce) (CSUC). Summit Co., Ten Mile Range Creek, avalanche chute, west side of peak 5, 17-vii-1998, 1 F (S. Fitzgerald) (CSUC). IDAHO: Beneway Co., Indian Cr., T44N R3W S30, 12-ix-1994, 1 L (M.D. Walters) (WFBM). Boise Co., small tributary to Mores Creek, Voqualin Gulch 28 km fm. Idaho City, 14-iv-1964, 4 M (SDS) (WFBM). Latah Co., White Pine Gulch, 14-iv-1962, 3 M, 1 F (SDS) (WFBM); Palouse River, White Pine Gulch, 14-v-1962, 2 M (SDS) (WFBM). Lemhi Co., Pine Creek, 3.2 km W Shoup, 01-vi-1966, 3 M, 1 F (SDS and E.R. Logan) (DRGPC). Shoshone Co., Jct. of Boulder & Marble Creeks, 19.3 km E Colder, 04-vii-1964, 1 M (SDS) (WFBM). Valley Co., Ditch Creek, 22.4 km N Landmark, 22- vii-1965, 1 L (SDS and E.R. Logan) (WFBM); Tindall Meadows 12.9 km S Landmark, 22-vii-1965, 2 M (SDS and E.R. Logan) (WFBM); S. Fork Salmon River, 12-vii-1978, 1 F (R.C. Biggams) (WFBM); Silver Creek, nr. Guard Station, 09-vi-1982, 1 M (L.R. Schoenike) (WFBM). MONTANA: Gallatin Co., Pine Creek, 5.6 km off Trail Creek Rd., (FS Rd. 978), 10-vi-1987, 1 M, 1 F (BCK) (CSUC); Grayling Creek, 14.5 km N of West Yellowstone, 09-vi-1989, 4 M, 3 F (BCK) (CSUC); Saint Regis River, at Hagun, exit 15, 22-iv-2006, 1 M (R. Durfee) (CSUC); Canyon Creek, at USFS trailhead W of Hamilton, 15-v-2008, 1 M (R. Durfee) (CSUC). Lincoln Co., Fairway and Stanley Creeks nr. Troy, ARASCO Mining, 21-x-1998, 5 L (RWW) (RWWPC); 21-x-1998, 1 L (RWW) (RWWPC). Mineral Co., St. Regis River at Hagun, exit 16, 22-iv-2006, 1 M (R. Durfee) (CSUC). NEVADA: Humboldt Co., Singas Creek, Singas Creek Cmpgd. nr. Paradise Valley, 16-vi-2009, 2 M (BCK and R. Baumann) (CSUC). OREGON: Baker Co., Elk Creek, 15.1 km SW Unity (Jct. of USFS Rds. 6005 & 2640 at Elk Creek Cmpgd.), Wallowa-Whitman Nat'l. Forest, 12-vii-2014; 10 M, 11 F (DRG) (DRGPC); Last Chance Creek, 19.3 km W of Unity (Jct. of USFS Rds. 263 & 050) (4.2 km NW Elk Creek Cmpgd.), Wallowa-Whitman Nat'l. Forest, 12- vii-2014, 6 M, 1 F (DRG) (DRGPC); S. Fork Burnt River, 12.8 km W of Unity (USFS Rd. 6005), Wallowa- Whitman Nat'l. Forest, 12-vii-2014, 2 M (DRG) (DRGPC). Clackamas Co., Crater Creek Spring, (Clackamas River tributary), 01-vi-2000, 6 M, 1 F (RWW) (RWWPC); Clackamas River, 10-x-2000, 4 L (RWW) (RWWPC). Douglas Co., French Creek at headwaters of S. Umpqua River, 31-v-1986, 1 M (Stansbury) (RWWPC). Bear Creek, Umpqua Nat’l. Forest, 27-x-2000, 10 L (RWW) (RWWPC); N. Umpqua River, Umpqua Nat’l. Forest, Crystal Springs, N43.521 W122.236, 08-viii-2015, 1 F P, 1 P, 1 PP (RWW) (RWWPC). Hood River Co., Polallie Creek, N45.416 W121.566, 08-vii-1986, 3 M (GWC) (RWWPC); Wyelth Creek, 20.9 km W Hood River (Hwy. 84), 05-v-1987, 2 M (RWW) (RWWPC). Jackson Co., Glade Creek, Rogue River Nat’l. Forest, Ashland Ranger District, 40S 1W Sec. 18, 07-v-2000, 4 M (RWW) (RWWPC); Yale Creek, Rogue River Nat’l. Forest, 40S 2W Sec. 23, 07-v-2000, 5 M (RWW) (RWWPC); Lower Gap Creek, Rogue River Nat’l. Forest, Ashland Ranger District, 40S 5W Sec. 34, 19-vi-2000, 9 F (RWW) (RWWPC); Jack Springs, N44.47664 W122.83754, 12-v-2014, 1 M (CDK) (DERPC); Wagner Creek, 9.5 km S of Talent, (Wagner Creek Rd.), N42.17016 W122.78410, 22-v-2014, 2 M, 3 F (BCK, CJV, J. Sandberg, and B. Stark) (CSUC). Jefferson Co., Dutchoven Creek, Medford District, BLM, 19-ix-2000, 24 L (RWW) (RWWPC); creek, nr. Jack Creek Cmpgd., Deschutes Nat’l. Park, 25-vii-2007, 1 M (F. Domlrosbi) (SMEC); Jack Springs, N44.47664 W121.72327, 12-v-2014, 1 M (CDK) (DERPC). Klamath Co., Crater Lake Nat’l. Park, Park HQ, 06-vii-1960, 2 M, 1 F (D. Huntzinger) (WFBM); Crater Lake Nat’l. Park, 06-vii- 1960, 2 M, 1 F (D. Huntzinger) (WFBM); 17-viii-1960, 1 L (D. Huntzinger) (WFBM); Sun Creek, Crater Lake Nat’l. Park, ix-1994, 3 L (RWW) (RWWPC). Lake Co., Bear Creek Cmpgd., N39. 32203 W122.83754, 14-iv- 2014, 12 M, 16 F (CDK) (DERPC); Marion Co., Clackamas River (USFS Rd. 46 crossing) N44.90 W121.883, 23- v-1987, 3 M, 1 F (GWC) (RWWPC). Wallowa Co., Gumboat Creek, 37.8 km SW of Jct. of County Rd. 39 & Hwy. 350 (which is 12.8 km. NE Joseph), Wallowa-Whitman Nat'l. Forest (Hells Canyon Rec. Area), 09-vii-2014, 4 M (DRG) (DRGPC); Lick Creek, 25.3 km SE of Jct. of County Rd. 39 & Hwy. 350 (which is 12.8 km NE Joseph), Wallowa-Whitman Nat'l. Forest, 09-vii-2014, 4 M, 1 F (DRG) (DRGPC); S. Fork Big Sheep Creek, 20 km SE of Jct. of County Rd. 39 & Hwy. 350 (which is 12.8 km NE Joseph), Wallowa-Whitman Nat'l. Forest, 09-vii-2014, 11 M (DRG) (DRGPC); small creek, 35 km SW of Jct. of County Rd. 39 & Hwy. 350 (which is 12.8 km NE Joseph), Wallowa-Whitman Nat'l. Forest (Hells Canyon Rec. Area), 09-vii-2014, 4 M (DRG) (DRGPC); Wallowa River, 8.8 km SE Joseph, Wallowa State Park, 07-vii-2014, 3 M (DRG) (DRGPC); Wallowa River, 10 km SE Joseph, Wallowa State Park, nr. Eagle Cap Trailhead, 07-vii-2014, 8 M (DRG) (DRGPC). WASHINGTON: Columbia Co., Little Tucannon River 48 km SE on Tucannon Rd. (F. S. Rd. 3127) off Hwy. 12., (Jct. of Tucannon Rd. & Hwy. 12 is 20 km E of Dayton), N46.2285 W117.7240, 27-v-2015, 2 M, 1 F (DRG) (DRGPC); Punjab Creek, 51.5 km S of Hwy. 12, USFS Rd. 4173 off Tucannon Rd., Umatilla Nat'l. Forest, N46.228 W117.724, 28-v-2015, 3 M, 1 F (DRG) (DRGPC); small creek, 51.5 km S of Hwy. 12, USFS Rd. 4173 off Tucannon Rd., Umatilla Nat'l. Forest, N46.2285 W117.7252, 28-v-2015, 5 M, 3 F, 1 L (DRG) (DRGPC); Tucannon River 51.1 km S of Hwy. 12 on Tucannon Rd., (USFS Rd. 4173) (Jct. of Tucannon Rd. & Hwy. 12 is 20 km E of Dayton, mile marker 31, km 49.8), Blue Mts., Umatilla Nat'l Forest, N46.193 W117.716, 28-v-2015, 11 M, 6 F (DRG) (DRGPC); N. Fork Touchet River 29 km SW Dayton, (Touchet Rd.) (Jct. of State Forest Service Rd. 9115 & USFS Rd. 64), Umatilla Nat'l. Forest, N46.118 W117.823, 29-v-2015, 1 M (DRG) (DRGPC); N. Fork Touchet River 28.8 km SW Dayton, (USFS Rd. 64), Umatilla Nat'l. Forest, N46.119 W117.821, 29-v-2015, 1 M (DRG) (DRGPC); N. Fork Touchet River, 30.5 km SW Dayton (Touchet Rd. 9115-USFS Rd. 64) (at Jct. of USFS Rd. 64 & Touchet Corral Trail), Umatilla Nat'l. Forest, N46.093 W117.847, 29-v-2015, 4 M (DRG) (DRGPC). Jefferson Co., HoH River, HoH River Ranger Station, Olympic Nat'l. Park, 17-vi-1974, 1 M (SDS) (DRGPC); HoH River, HoH River Ranger Station, Olympic Nat'l. Park, 14-v-1974, 1 M (SDS) (DRGPC). Kittitas Co., Reeser Creek, 25.7 km N of Ellensburg (Reeser Creek Rd.), 14-iv-1974, 1 P, 2 PP (DRG) (DRGPC); 12-vi-2012, 1 M P (DRG) (DRGPC). Lewis Co., Middle Fork Johnson Creek, N46.50 W121.55, 18-vi-1986, 1 M (GWC) (RWWPC); Ohanapecosh River, N Silver Falls (Hwy. 123), Mt. Rainier Nat'l. Park, 23-v-2003, 4 M, 1 F (BCK and J. Schmidt) (CSUC); White River, (Hwy. 410), Mt. Rainier Nat'l. Park, 23-v-2003, 1 L (BCK and J. Schmidt) (CSUC); Clear Fork Cowlitz River 26.5 km E Packwood (2.4 km W of White Pass), Gifford-Pinchot Nat'l. Forest, N46.627 W121.425, 20-v-2015, 6 M, 1 F, 1 L (DRG) (DRGPC); Coal Creek, 4.5 km E Packwood (Hwy. 12), N46.650 W121.610, 20-v- 2014, 3 M, 7 F (DRG) (DRGPC); Lake Creek, 3.05 km E Packwood, N46.634 W121.642, 20-v-2015, 5 F (DRG) (DRGPC); Smith Creek, 5.3 km W of Packwood, (Hwy. 12) (Baker Rd. off Smith Creek Rd.), N46.5696 W121.7097, 20-v-2015, 6 M (DRG) (DRGPC); creek, 7.5 km E Randle (Hwy. 12, W of mile marker 122, km 196), N46.535 W121.817, 23-v-2015, 4 M, 1 F (DRG) (DRGPC); Yellowjacket River, just below confluence with Pinto Creek, 10-vi-1986, 3 M, 2 F (GWC) (RWWPC); Yellowjacket River, (Cispus Rd. off County Rd. 23) 15 km S of Randle, Gifford-Pinchot Nat'l. Forest, N46.432 W121.839, 23-v-2015, 2 M (DRG) (DRGPC). Pierce Co., Longmire, Mt. Rainier Nat’l. Park, 23-v-1986, 6 M, 2 F (Hawkins) (RWWPC); 21-vi-1986, 1 M, 2 F (Hawkins) (RWWPC); 31-vii-1986, 14 F (Hawkins) (RWWPC); 09- viii-1986, 7 M (Hawkins) (RWWPC); White River at Cmpgd., Mt. Rainier Nat’l. Park, 14-viii-1986, 9 M, 4 F (Furnish) (RWWPC); Frying Pan Creek, Mt. Rainier Nat'l. Park, 24-v-2003, 9 PP (BCK, J. Schmidt, and J. Evans) (CSUC); Panther Creek, (Hwy. 123), Mt. Rainier Nat'l. Park, 24-v-2003, 2 L (BCK, J. Evans and J. Schmidt) (CSUC). Skagit Co., Stetatlle River at mouth of River, North Cascades Nat’l. Park, 20-xi-2013, 7 L (RWW) (RWWPC). Snohomish Co., Whitechuck River, N48.183 W121.183, 31-vii-1986, 1 M, 5 F (GWC) (RWWPC). Whatcom Co., Gallop Creek, ca. km 53.9 (mile marker 33.5) on Hwy. 542, Mt. Baker, N48.890 W121.943, 03-v-2010, 1 M (D.W. Blinn) (DERPC); Canyon Creek, on Mosquito Creek Rd., N48.834 W121.137, 18-vi-2010, 3 M (D.W. Blinn) (DERPC). WYOMING: Big Horn Co., E. Fork Little Goose Creek, Cloud Peak Wilderness, Big Horn Nat’l. Forest, 02-ix-2007, 52 L, (RWW) (RWWPC); Fremont Co., Stough Creek, 03-ix-2003, 20 L (REZ) (CSUC). Teton Co., Blackrock Creek, E of Blackrock, 03- vi-1988, 1 M (BCK) (CSUC).

Figures

FIGURES 1a–1c. Ecclisomyia bilera Denning, male genitalia. 1a, left lateral; 1b, ventral; 1c, dorsal; Abbreviations: ifa = inferior appendage (paired); ima = intermediate appendage; IX = segment IX; par = paramere (paired); phcr = phallocrypt; pl = pleuron (paired); sa = superior appendage (paired); VIIIs = sternum VIII; VIIIt = tergum VIII; IXt = tergum IX; Xt = tergum X.

FIGURES 2a–2c. Ecclisomyia bileraDenning, male genitalia.2a, left lateral; 2b, left caudodorsal; 2c, ventral; Abbreviations: ifa = inferior appendage (paired); ima = intermediate appendage; IX = segment IX; par = paramere (paired); ph = phallus; phcr = phallocrypt; pl = pleuron (paired); sa = superior appendage (paired), VIIIs = sternum VIII; VIIIt = tergum VIII; Xt = tergum X.

FIGURES 5a, 5b. Ecclisomyia bilera Denning, male pupa, apical processes.5a, dorsal; 5b, ventral. FIGURES 6a, 6b. Ecclisomyia bilera Denning, male pupa, apical processes.6a, dorsal; 6b, ventral.

FIGURES 6a, 6b. Ecclisomyia bilera Denning, male pupa, apical processes.6a, dorsal; 6b, ventral.

FIGURES 10a–10c. Ecclisomyia conspersa Banks, male genitalia. 10a, left lateral; 10b, ventral; 10c, dorsal. Abbreviations: ifa= inferior appendage (paired); ima = intermediate appendage; IX= segment IX; par = parameres (paired); ph = phallus; phcr = phallocrypt; pl = pleuron (paired); sa = superior appendage (paired); VIIIs = sternum VIII; VIIIt = tergum VIII; Xt = tergum X.

FIGURES 12. Ecclisomyia conspersa Banks, female genitalia, ventral. Abbreviations: e.gon.VIII = external part of gonopod VIII; e.gon.IX = external part of gonopod IX; spt.sc = spermathecal sclerite; ssc.o = spermathecal sclerite opening; VIIIs = sternum VIII; IXs = sternum IX; Xt = tergum X.

FIGURES 15a, 15b. Ecclisomyia conspersa Banks, male pupa,apical processes.15a, dorsal; 15b, ventral. FIGURES 16a, 16b. Ecclisomyia conspersa Banks, male pupa,apical processes.16a, dorsal; 16b, ventral.

FIGURES 17a, 17b. Ecclisomyia conspersa Banks, female pupa, apical processes.17a, dorsal; 17b, ventral. FIGURES 18a, 18b. Ecclisomyia conspersa Banks, female pupa, apical process.18a, dorsal; 18b, ventral.

FIGURES 19a, 19b. Ecclisomyia conspersa Banks, larval head. 19a, dorsal, with primary setae numbered; 19b, ventral, with primary setae numbered.

FIGURES 20a–20d. Ecclisomyia conspersa Banks, pupa. 20a, labrum, dorsal; 20b, mandibles, ventral; 20c, abdominal setae and hook plates, dorsal;20d, dorsal process abdominal segment I, dorsal.

FIGURES 21a–21c. Ecclisomyia conspersa Banks, larva, right legs. 21a, prothoracic leg; 21b, mesothoracic leg; 21c, metathoracic leg.Abbreviations:ls = long slender setae; sl = spike-like setae; tb = trochanteral brush.

FIGURES 25a–25c. Ecclisomyia maculosa Banks, female terminalia. 25a, left lateral; 25b, ventral; 25c, ventral. Abbreviations: e.gon.VIII = external part of gonopod VIII; e.gon.IX = external part of gonopod IX; spt.sc = spermathecal sclerite; ssc.o = spermathecalsclerite opening; VIIIs = sternum VIII; IXs =sternum IX; Xt = tergum X.

FIGURES 26a, 26b. Ecclisomyia maculosa Banks, male pupa, apical process.26a, dorsal; 26b, ventral.

FIGURES 27a, 27b. Ecclisomyia maculosa Banks, male pupa, apical process.27a, dorsal; 27b, ventral.

FIGURES 30a–30c. Ecclisomyia simulata Banks, male genitalia. 30a, left lateral; 30b, ventral; 30c, dorsal. Abbreviations: ifa = inferior appendage (paired); ima = intermediate appendage;IX = segment IX; par = paramere (fusedpair); ph = phallus; phcr = phallocrypt; pl = pleuron (paired); sa = superior appendage (paired); VIIIs = sternum VIII; VIIIt = tergum VIII; Xt = tergum X.

FIGURES 31a–31c. Ecclisomyia simulata Banks, male genitalia. 31a, left lateral; 31b, ventral; 31c, dorsal. Abbreviations:ifa = inferior appendage (paired); ima = intermediate appendage; IX = segment IX; par = parameres (fused pair); ph = phallus; phcr = phallocrypt; sa = superior appendage (paired); VIIIs = sternum VIII; VIIIt= tergum VIII; Xt = tergum X.

FIGURES 32a–32c. Ecclisomyia simulata, female genitalia. 32a, ventral; 32b, ventral; 32c, left lateral. Abbreviations: e.gon.VIII = external part of gonopod VIII; e.gon.IX = external part of gonopod IX; spt.sc = spermathecal sclerite; ssc.o = spermathecal sclerite opening; VIIIs = sternum VIII; IXs = sternum IX; Xt = tergum X.

FIGURES 33a, 32b. Ecclisomyia simulata Banks, male pupa, apical process. 33a, dorsal; 33b, ventral.

FIGURES 34a, 34b. Ecclisomyia simulata Banks, male pupa, apical process. 34a, dorsal; 34b, ventral.

FIGURE 41. Pupal schematic hook plate diagrams, dorsal, for Ecclisomyia bilera Denning, Ecclisomyia conspersa Banks, Ecclisomyia maculosa Banks, Ecclisomyia simulata Banks. Abbreviations: I–VIII = dorsal abdominal segments, with Arabic numerals representing the average number of hooks per plate.

FIGURE 42. Pupal and larval gill schematic diagrams, left lateral, for Ecclisomyia bilera Denning, Ecclisomyia conspersa Banks, Ecclisomyia maculosa Banks, Ecclisomyia simulata Banks. Abbreviations: I–VIII = dorsal abdominal segments, with Arabic numerals representing the number of gills per segment in anterior and posterior positions at dorsal, lateral or lateral line (dorsolateral and ventrolateral), and ventral levels.

FIGURES 43a–43d. Pupal cases. 43a-1 and 43a-2, Ecclisomyia bilera Denning (8–11 mm); 43b, Ecclisomyia conspersa Banks (16 mm); 43c, Ecclisomyia maculosa Banks (10.5 mm); 43d, Ecclisomyia simulata Banks (11 mm).

FIGURES11a–11c. Ecclisomyia conspersa Banks, male genitalia.11a, left lateral; 11b, ventral; 11c, dorsal. Abbreviations:ifa = inferior appendage (paired); ima = intermediate appendage; IX = segment IX; par = paramere (paired); ph = phallus; phcr = phallocrypt; pl = pleuron (paired); sa = superior appendage (paired); VIIIs = sternum VIII; VIIIt = tergum VIII; Xt= tergum X.

FIGURES22a–22c. Ecclisomyiamaculosa Banks, male genitalia.22a, left lateral; 22b,ventral; 22c,dorsal. Abbreviations:ifa = inferior appendage (paired); ima = intermediate appendage; IX = segment IX; par = parameres (fused pair); phcr = phallocrypt; pl = pleuron (paired); sa = superior appendage (paired); VIIIs = sternum VIII; VIIIt = tergum VIII; Xt= tergum X.

FIGURES23a–23c. Ecclisomyiamaculosa Banks, male genitalia.23a, left lateral; 23b, ventral; 23c, dorsal. Abbreviations:ifa = inferior appendage (paired); ima = intermediate appendage; IX = segment IX; par = parameres (fused pair); ph = phallus; phcr = phallocrypt; pl = pleuron (paired); sa = superior appendage (paired); VIIIs = sternum VIII; VIIIt = tergum VIII; Xt = tergum X.

FIGURES13a, 13b. EcclisomyiaconspersaBanks, femalegenitalia.13a, ventral;13b, left lateral.Abbreviations:e.gon.VIII = external part of gonopod VIII; e.gon.IX = external part of gonopod IX; spt.sc = spermathecal sclerite; ssc.o = spermathecal sclerite opening; IXs = sternum IX; Xt = tergum X.

FIGURES24a–24c.Ecclisomyia maculosa Banks, female terminalia.24a, leftlateral; 24b, dorsal;24c, ventral.Abbreviations: e.gon.VIII = external part of gonopod VIII; e.gon.IX = external part of gonopod IX; IXs = sternum IX; IXt = tergum IX; pl = pleuron (paired); spt.sc = spermathecal sclerite; ssc.o = spermathecal sclerite opening; VIIIs = sternumVIII; IXs = sternum IX; IXt = tergum IX; Xt = tergum X.

FIGURES 37, 38. Ecclisomyia spp., pupal hook plates. 37, Ecclisomyia bilera Denning; 38, Ecclisomyia conspersa Banks. Abbreviations: III–VII = abdominal terga III through VII; a = anterior; p = posterior.