Sphingobacterium prati, Besaury & Floret & Rémond, 2021

Besaury, Ludovic, Floret, Juliette & Rémond, Caroline, 2021, Sphingobacterium prati sp. nov., isolated from agricultural soil and involved in lignocellulose deconstruction, International Journal of Systematic and Evolutionary Microbiology (004963) 71 (8), pp. 1-8 : 7

DESCRIPTION OF SPHINGOBACTERIUM PRATI SP. NOV.

Sphingobacterium prati (pra'ti L. gen. n. prati of a meadow).

Cells are Gram-stain-negative, non-motile, non-sporeforming, strictly aerobic rods, 0.8–1.7 µm long and 0.7–0.9 µm wide. After 2 days incubation on LB agar, colonies are 1.0– 2.5 mm in diameter, slightly yellowish, convex, circular and smooth. Growth occurs at 15–37 °C (optimum, 30 °C), pH 6.0–9.0 (optimum, pH 7.0) and with 0–4% NaCl (optimum, 0 %). Growth occurs on nutrient agar and LB agar, but not on MacConkey agar. Positive for catalase and oxidase. Acid is produced from sodium lactate, acetoacetic acid, cellobiose, dextrin, D-fructose,D-galactose, D-galacturonic acid,D-gluconic acid, maltose, D-mannose, melibiose, D-salicin, turanose, gelatin, gentiobiose, glycyl-L-proline, guanidine HCl, L-fucose, L-rhamnose, N -acetyl-D-galactosamine, N -acetyl-β-D-mannosamine, nalidixic acid, pectin, sodium butyrate, sucrose, D-glucose and lactose. Acid is not produced from 3-methyl-glucose, bromo-succinic acid, citric acid, D-arabitol,D-aspartic acid, D-fructose-6- PO 4 , D-fucose,D-glucose-6- PO 4 , D-gluronicacid, D-lactic acid, methyl ester, D-malic acid,D-mannitol, raffinose, D-saccharic acid,D-serine,D-sorbitol, trehalose, formic acid, fusidic acid, glucuronamide, glycerol, inosine, L-alanine, L-arginine, L-aspartic acid, L-galactonic acid, lactone, L-glutamic acid,L-histidine,L-lactic acid,L-malic acid, L-pyroglutamic acid,L-serine, methyl pyruvate, mucic acid, myo-anositol, N -acetyl neuraminic acid, N -acetyl-Dglucosamine, p -hydroxy-phenylacetic acid, propionic acid, quinic acid, stachyose, Tween 40, α-hydroxy-butyric acid, α-keto-butyric acid, α-keto-glutaric acid, β-hydroxy-D acid, L-butyric acid, methyl β-D-glucoside and γ-amino-butyric acid. Resistant to aztreonam, rifamycin and sensitive to lincomycin, minocycline, troleandomycin and vancomycin. Strain arapr2 T harbours β-galactosidase but not lysine decarboxylase, ornithine decarboxylase, urease and tryptophane desaminase ( API 10 S). Strain arapr2 T can oxidize glucose and produce NO 2 but cannot oxidize arabinose or produce H 2 S ( API 10 S). The predominant fatty acids are iso-C 15:0, C 16:0 and C 17:0 iso 3-OH. Menaquinone-7 is the major respiratory quinone. The major polar lipids are glycolipid, aminolipid, phospholipid, phosphatidylethanolamine, phosphoaminolipid and phosphoaminoglycolipid.

The type strain, arapr2 T (CIP 111872 T =LMG 31848 T), was isolated from meadow soil collected near Reims , France. The DNA G+C content of the type strain is 40.4 mol%.