Phaeoacremonium croatiense Essakhi, Mugnai, Surico & Crous, Persoonia

Shang, Qiu-Ju, Hyde, Kevin D. & Liu, Jian-Kui, 2021, Phaeoacremonium fusiformostromum sp. nov. and a new record of P. croatiense from China, Phytotaxa 516 (1), pp. 59-72 : 68

Phaeoacremonium croatiense Essakhi, Mugnai, Surico & Crous, Persoonia 21: 127 (2008) FIG. 3

MycoBank number: MB 506947; Facesoffungi number: FoF 09707

Sexual morph: Ascomata 120–209 μm high, 195−309 μm diam., perithecial, solitary or scattered, immersed in decaying wood, becoming raised to erumpent by perithecial neck, dark brown to black, glabrous, subglobose, ostiolate. Perithecial necks 29–60 μm high, 45–70 μm diam., cylindrical, ostiolar canals sulcate, periphysate. Peridium 20–41 μm wide, composed of two section layers, outer section comprising 5–7 layers, of dark brown to black, thick-walled cells, arranged in a textura angularis, inner part comprising 1–2 layers of subhyaline to hyaline flattened cells of textura angularis. Hamathecium composed of cylindrical, septate, hyaline, paraphyses, 2.9–4 μm wide near the base, slightly inflated between the septa near their base, longer than the asci. Asci (12–)15.5–19(–22) × (3.5–)4–5(–5.5) μm (x = 17.3 × 4.4 μm, n = 100), arising in acropetal succession, 8-spored, unitunicate, cylindrical to clavate, short stalked, apically rounded to truncate. Ascogenous hyphae septate, hyaline, branched. Ascospores (3.3)3.8–4.8(–6) × (1–)1.3–1.7(–2) μm (x = 4.3 × 1.5 μm, n = 100), overlapping 2–3-seriate, hyaline, oblong to allantoid, aseptate, smooth-walled, with small guttules at the ends. Asexual morph: Undetermined

Material examined:— CHINA, Guizhou Province, Zunyi, Kuankuoshui natural reserve, on undetermined dead wood, 16 September 2018, Q. J . Shang, KKS 19 ( HKAS 111964 ; MFLU 19-1894 ) .

GenBank numbers: LSU = MZ 227125 , ITS = MZ 227131 , SSU = MZ 227128 , RPB2 = MZ 231119 .

Notes:—Our collection is identical to Phaeoacremonium croatiense based on morphology and phylogenetic evidence ( FIGS. 1 , 3 ). To date, only the asexual morph has been reported for the species and our study provides the first sexual morph. In addition, Phaeoacremonium croatiense is only known in Croatia ( Essakhi et al. 2008) and this is the first record for China.

Figures

FIGURE 1. Phylogram generated from MP analyses based on a combined dataset of ACT and TUB2 sequence data. Bootstrap support values for ML (back) and MP (red) equal to or greater than 75% are shown above the nodes. The BI values greater than 0.95 are indicated by blue triangle. The new isolates are in blue and ex-type strains are in bold. The tree was rooted with Pleurostoma richardsiae (CBS 270.33).

FIGURE 3. Phaeoacremonium croatiense (HKAS 111964). a, b Appearance of ascomata on the substrate.c Cross section through ascoma. d, e Vertical section through ascomata (e with ostiolar canal). f Peridium. g Paraphyses and ascogenous hyphae with asci attached. h, i, k Ascogenous hyphae with asci attached. l, m Asci. j Paraphyses. n Germinating ascospore. o Ascospores. p, q Culture characteristic on PDA after 10 days (p = colony from above, q = colony from below). Scale bars: d, e = 50 µm, f = 20 µm, g–j, n = 10 µm, l, m, o = 5 µm.