Stilobezzia coquilletti Kieffer 1917

Ronderos, María M., Cazorla, Carla G., Spinelli, Gustavo R. & Carrasco, Daiane Silveira, 2008, Description of immature stages and adult diagnosis of Stilobezzia coquilletti Kieffer 1917 (Diptera: Ceratopogonidae), Zootaxa 1958, pp. 31-40 : 32-36

Stilobezzia coquilletti Kieffer 1917

Figs. 1–35

Ceratopogon pictus Coquillett, 1905: 60 (female; USA, Virginia). Preoccupied by C. pictus Meigen.

Hartomyia picta (Coquillett) : Malloch, 1915: 341 (male; USA, Illinois; comb.).

Stilobezzia coquilletti Kieffer, 1917: 308 (new name for C. pictus Coquillett ); Johannsen, 1943:781 (distrib.); Johannsen, 1952: 162 (in key); Wirth, 1953: 75 (male descr., female redesc.; distrib.); Woke, 1954: 69 ( Panama; notes); Lane & Forattini, 1956: 213 (adult redesc.; Neotropical distrib.); Lane & Forattini 1961: 85 (pupa descr.; distrib.); Wirth & Grogan, 1981: 73 (adult, pupa redescr.; biology; distrib.); Borkent & Wirth, 1997: 110 (World catalog); Borkent & Spinelli, 2000: 54 (south USA catalog); Borkent & Spinelli, 2007: 30 (Neotropical catalog).

Diagnosis of Adult ( Figs. 1–5, 34, 35). A yellowish species of the subgenus Stilobezzia s. str. Female flagellomeres pale except apices brown; palpus dark brown; scutum yellowish, anterior mesal margin darker, with two anterolateral and two posterolateral brown spots; wing hyaline with small brown spots over crossvein r-m, apex of R 3 and large brown spot in r 3 below apex of second radial cell; one ovoid spermatheca with short neck; gonocoxites connected by a strong bar dorsal to aedeagus; parameres stout, connected by transversal bar, each one with basal portion highly sclerotized, main portion stout with subapical, pointed projection, tip spinelike; aedeagal sclerites slightly sinuous, with blunt, hairy tip.

Description of fourth instar larva ( Figs. 8–19). Head capsule ( Figs. 8, 13–15) pale brown, elongate, tapering to rounded apex; chaetotaxy as in Figs.13–14; HL 0.23 mm (SEM), 0.25 mm (BCM); HW 0.15 mm (SEM), 0.13 (BCM); HR 1.35 (SEM), 1.92 (BCM); SGW 0.10 mm (SEM), 0.09 mm (BCM); SGR 1.50 (BCM). Labrum ( Figs. 8, 13, 15) as long as greatest breadth; palatum ( Figs. 15, 18) with three pairs of sensilla styloconica (one large, two short) on its anterior edge, pair of sensilla trichoidea immediately underneath ( Figs. 18, 19); messors ( Figs. 16, 18, 19) small, thin, curved, well sclerotized; well developed palatal bar ( Figs. 18, 19). Maxilla ( Figs. 15, 17) sclerotized, with short, slender papilla; galeolacinia with bundle of four setae; maxillary palpus cylindrical, with 3–4 papillae of different length, flaplike lobe lying alongside. Mandible ( Figs. 8, 11, 15) bidentate, thin, curved, strongly sclerotized; well defined, long, apical tooth, proximal tooth small; evident fossa mandibularis on ectal surface, MDL 0.07 mm. Hypostoma ( Figs. 8, 15–18) with smooth mesal elevation flanked by serrate margins, with 8–9 teeth. Epipharynx ( Figs. 8–10) massive with three combs; comb 4 massive, its posterior margin irregular, with 9–10 lanceolate, subequal, mesal teeth, lateral teeth strong, pointed, largest than mesal ones; other two combs superimposed, reduced, not in position to be observed; dorsal comb sclerites with irregular posterior margin with 9–10 lanceolate teeth; each lateral arm membranous, without curtain or fringe; LAW 0.11 mm, DCW 0.07 mm. Hypopharynx ( Fig. 8) robust, thick, posterior end of each arm irregular, hipopharingeal fringe no visible. Thoracic pigmentation not visible. Caudal segment wide, chaetotaxy as in Fig. 12; one pair of subequal, medium-sized, thin setae “o”; CSL 0.30 mm, CSW 0.18 mm, OD 0.08 mm.

Description of pupa ( Figs. 20–33). Exuviae pale brown. Operculum ( Figs. 20, 26), hexagonal, disk smooth; anteromarginal tubercle (am) ( Fig. 26) strong, elongate, with stout spur-like, basal sensillum; OL 0.08 mm; OW 0.13 mm; OW/OL 1.68. Thorax surface smooth, with medial crest extending between bases of respiratory organs. Thoracic tubercles as follows: anterodorsal tubercle (ad) ( Figs. 21, 31) with one mediumsized stout seta, one basal pore, wide base; dorsolateral tubercle (dl) ( Figs. 24, 31) with two setae, one long, thin, hyaline, other stout, short, flattened base; 5 dorsal sensilla (d) ( Fig. 21): i pore, ii with stout mediumsized seta, tringular base, iii without seta, raised base, iv small flattened base, v pore; two dorsomedial hyaline setae (dm) ( Figs. 22, 31), one long, thin, triangular base, other medium-sized, flattened base. Respiratory organ ( Figs. 20, 23, 30) pale brown, dark brown at tip, long, slender, nearly parallel sides, nearly straight, rounded apex; 12–14 apical pores, 4–5 basal pores opening at tubercles, length 0.38 (0.36–0.39, n=3)mm; pedicel dark brown, smooth, stout, wide base; length 0.16 (0.14–0.19, n=3) mm, P/RH 0.44 (0.41–0.48, n=3). Two ventromedian setae (vm), only insertions visible, 2 ventrolateral setae (vl), one stout, medium-sized, other pore ( Figs. 20, 28). Abdominal segments integument with narrow band of sharp spinules on anterior margin, tubercles strong, spiniform, with well sclerotized apex; fourth abdominal segment tubercles ( Figs. 20, 27, 32, 33) as follows: 5 dorsoposteromarginal tubercles (dpm), i–ii with minute seta, rounded base, iii–iv with thin, minute setae, conical base, v with short strong seta, conical base; 2 dorsoanterosubmarginal tubercles (dasm), i small triangular base, ii strong triangular base, i–ii with short, thin seta; 2 lateral anteromarginal tubercles (lasm), i–ii pore; 3 lateral posteromarginal tubercles (lpm), i–iii with short, strong seta, conical base; 3 ventroposteromarginal tubercle (vpm), i with strong, short seta, conical, strong, wide base, ii with thin, medium-sized seta, doble conical base, iii with short, thin seta, minute rounded base. Female anal segment ( Figs. 20, 29) dorsal surface densely covered at base with posteriorly directed, pointed spicules, apicolateral process triangular, smooth, long, pointed, wide base, directed laterally, extreme tips darkish; length 0.21 (0.21–0.22, n=3) mm, width 0.16 (0.14– 0.18 n =3) mm. Male anal segment as in female, length 0.24 (0.20–0.28, n=3) mm, width 0.15 (0.14–016, n=3) mm.

Distribution. USA (Virginia, Illinois, Maryland), Mexico (Chiapas, Yucatán) to Panama, Trinidad, Brazil (Manaus, Sao Paulo)

Specimens examined. Brazil, Manaus , San Jose, 03°03´48.1”S, 59°57´46.1”W), 13-X-2005, D. Silveira Carrasco, 5 females (3 with pupal exuviae), 5 males (3 with pupal exuviae), 1 larva exuviae .

Specimens examined with SEM. Brazil, Manaus, San Jose, 13-X-2005, D. Silveira Carrasco, 2 larvae .