Pleurotheciella sympodia H. Yang & H. Zhang, 2021

Shi, Lin, Yang, Hao, Hyde, Kevin D., Wijayawardene, Nalin N., Wang, Gen-Nuo, Yu, Xian- Dong & Zhang, Huang, 2021, Freshwater Sordariomycetes: new species and new records in Pleurotheciaceae, Pleurotheciales, Phytotaxa 518 (2), pp. 143-166 : 151

Pleurotheciella sympodia H. Yang & H. Zhang , sp. nov.

Index Fungorum number: IF557776, Facesoffungi number: FoF 08701

Etymology: Referring to the sympodial conidiogenous cells.

Holotype: MFLU 19-0552 , ex-type strain KUMCC 19-0213

FIGURE 3

Description: Saprobic on submerged decaying wood. Sexual morph Undetermined. Asexual morph Colonies on natural substrata effuse, scattered, hairy, solitary or in groups, with white masses of conidia on the upper part of dark brown conidiophores. Mycelium mostly immersed, partly superficial. Conidiophores macronematous, synnematous, septate, erect, mid brown to dark brown, smooth, 135–355 μm (x = 243 μm, n = 30) long, 1.5–3.5 μm (x = 2.5 μm, n = 30) wide. Synnemata erect, rigid, brown, velvety, smooth, conidiophores splaying out or divergent at the apical part, 7–22 μm (x = 14 μm, n = 30) wide. Conidiogenous cells polyblastic, terminal, integrated, smooth, cylindrical or subulate, subhyaline to pale brown, denticulate, with several tiny sympodial denticles. Conidia clavate, straight or slightly curved, rounded at apex, tapering towards base, with a single median septum, hyaline, guttulate, smoothwalled, 22.5–29 × 4.5–6.5 μm (x = 25 × 5.5 μm, n = 20).

Cultural characteristics: Conidia germinated on PDA within 24 hours. Germ tubes produced from the apex. Colonies on PDA slow-growing, reaching 9–10 diameters in 30 days at 25°C, with dense, humid, dark brown mycelium on the surface, in reverse dark brown to black, with undulate margin.

Material examined: THAILAND. Nakhon Phanom Province, a small river in That Phanom, on dead submerged decaying wood of unidentified plants, 12 December 2018, Hao Yang , t46 ( MFLU 19-0552 , holotype), ex-type living culture KUMCC 19-0213 ; THAILAND. Chiang Mai Province, a small river in Mae Ai , on dead submerged decaying wood of unidentified plants, 15 February 2018, Xian-Dong Yu, Y 17 ( MFLU 18-0995 , paratype), ex-paratype living culture MFLUCC 18-0983 ; THAILAND. Phayao Province, a small stream in Pong , on dead submerged decaying wood of unidentified plants, 23 February 2018, Gen-Nuo Wang, QR 3 ( MFLU 18-0965 , paratype), ex-paratype living culture MFLUCC 18-0658 .

Notes: In the phylogenetic tree, Pleurotheciella sympodia clustered within Pleurotheciella and was close to Pl. tropica and Pl. krabiensis .However, Pl.sympodia differs in having long (up to 355μm long), synnematous conidiophores and longer conidia (22.5–29 μm in Pl. sympodia vs. 16–21 μm in Pl. tropica vs. 19–25 μm in Pl. krabiensis ), while Pl. tropica has solitary conidiophores, and Pl. krabiensis has caespitose to loosely fascicled conidiophores ( Hyde et al. 2018). Thus, a new species, Pl. sympodia , is introduced herein. The strains Pl. sympodia ( MFLUCC 18-0983) and Pl. sympodia ( MFLUCC 18-0658) have identical ITS sequence data, which have four nucleotide differences from the ex-type strain Pl. sympodia ( KUMCC 19-0213).

Figures

FIGURE 3. Pleurotheciella sympodia (MFLU19-0552, holotype) a, b Colonies on natural substrate. c, d Conidiophores with conidia. e, f Conidiogenous cells with conidia. g–j Conidia. k Germinating conidium on PDA. m, n Culture on PDA from above and reverse. Scale bars: a, b = 100 μm, c, d = 50 μm, e, f, k = 20 μm, g–j = 10 μm.