Charinus guto, Giupponi & Miranda, 2016

Giupponi, Alessandro Ponce de Leão & Miranda, Gustavo Silva de, 2016, Eight New Species of Charinus Simon, 1892 (Arαchnidα: Amblypygi: Chαrinidαe) Endemic for the Brαziliαn Amαzon, with Notes on Their Conservαtionαl Stαtus, PLoS ONE (e 0148277) 1892 (2), pp. 1-33 : 13-15

Charinus guto new species. urn:lsid:zoobank.org:act:8E381891-5040-4040-BA37-2BB0A067A10F

( Figs 7A–7E , 2D, 3D and 4D)

Etymology. This species is named after the arachnologist José Augusto Pereira Barreiros, nicknamed Guto (in memoriam), who collected some of the specimens of the type series.

Type material. Holotype: BRAZIL: Pará: Belém, Bosque Rodrigues Alves , 27.x.2002, R. Pinto-da-Rocha leg. (female, MZSP 48146 ) . Paratypes: BRAZIL: Pará: Belém, Bosque Rodrigues Alves , 27.x.2002, R. Pinto-da-Rocha leg. (3 females, MZSP 48146 ) ; 27.x.2002, R. Pinto-da-Rocha leg. (1 female, MNRJ 09188 ) ; 08-XI-2001, J.A.P Barreirros (1 female juvenile, MPEG 059 ) ; 08.xi.2001, J.A.P Barreirros (1 female, MNRJ 09202 ) ; 08.iv.2001, A.B. Bonaldo et al. leg. (1 female, MPEG 037 ) ; 08.iv.2001, A.B. Bonaldo et al. leg. (1 female, MPEG 038 ) .

Diagnosis. Absent median eyes and tubercle; well-developed lateral eyes, but pale; small and rounded meta and mesosternum; small basal spine of the distitarsus of the pedipalp, ¼ the length of the distal; tibia I with 21 articles in the and tarsus with 37; basitibia of leg IV divided in three pseudo articles; trichobothria of the basitibia IV (bt) at the proximal third of the article; distitibia IV with 14 trichobothria; equidistant basal trichobothriae of distitibia IV (bf, bc and sbf); light brown body color; cushion-like gonopods with lateral projections directed backwards covering the aperture of the internal seminal receptacles.

Description. Carapace ( Fig 7A ): flattened, wider than long, with an anterior depression in place of the absent median eye tubercle, from which starts a thin median furrow that reaches around the posterior area of the pair of lateral hump, situated behind the lateral eye spots. Anterior margin with 6 small setae. Many tiny punctuations, more abundant in the frontal area. Punctuations arranged in lines and spots, irradiating from the fovea and interspersed with glabrous areas. Three pairs of deep furrows, and a very rectangular deep fovea. First pair of furrows just behind the lateral boss not reaching the middle line. 4 lateral pairs of depressions (first one placed over the 1 st pair of furrows). Lateral eyes well developed. Frontal process well developed, much longer than larger, with blunt, reborded apex.

Sternum ( Fig 7B ). Same as C. brescoviti sp. n. Abdomen ( Fig 7A ): same as C. brescoviti sp. n.

Chelicera ( Fig 2D ). Cheliceral furrow with 4 internal teeth, the distal one bifid, the first cusp bigger than the second one. Fourth twice as long as the others and much stouter. Teeth length: IV>Ia>Ib = II>III. Claw with 5 denticles, decreasing in size.

Pedipalp. Trochanter ( Fig 7D and 7E ): ventral apophysis large, at the posterior border of the article, spiniform, bearing many strong setae, with a blunt tip pointed forwards, and 2 subequal spines, one at the median third and the other at the distal tip of the prolateral face. Femur ( Fig 7D and 7E ): 3 dorsal spines decreasing in size from proximal to distal; two small setiferous tubercle before spine I; 3 ventral spines (some specimens with 4), same relation of size as the dorsal, slightly larger than the dorsal. Tibia ( Fig 7D and 7E ): 3 dorsal spines (III>II>I). Spine II 1/3 spine I; spine III 2/3 II. 1 small setiferous tubercle closes to spine I, and one after spine III. 2 ventral spines, basal 1/3 the distal. Basitarsus ( Fig 7C, 7D and 7E ): 2 dorsal spines, basal ½ the distal. 1 ventral spine at the distal half, slightly bigger than the basal

doi:10.1371/journal.pone.0148277.g007

dorsal. Distitarsus ( Fig 7C ): long, with 2 curved spines, basal ½ distal. Cleaning organ about ½ the article length. Claw ( Fig 7C ): long, with an acute, curved tip.

Legs. Same as C. brescoviti sp. n. Femur length I>III>IV>II. Tibia I with 21 articles. Tarsus (basitarsus+distitarsus) I with 37 articles. Leg IV: Basitibia: 3 pseudo-articles, one distal trichobothrium on the proximal pseudo-article, and 1 basal trichobothrium on the distal pseudo-article. Distitibia ( Fig 3D ): 3 basal and 13 distal trichobothria; bc is equidistant to bf and sbf ( Fig 3D ). Basitibia-distitibia length BT1>DT>BT3 = BT4>BT2. Basitarsus / distitarsus ratio 7/4, distitarsus tetramerous.

Measurements. Females (n = 3): Cephalothorax: Length: 1.887 mm (1.73–2.13), Width: 2.47 mm (2.35–2.61). Abdomen: 4.06 mm (3.7–4.5). Pedipalp: Femur 1.13 mm (1.09–1.17), Tibia 1.09 mm (1.04–1.13), Basitarsus 0.68 mm (0.64–0.72), Distitarsus 0.45 mm (0.43–0.48), Tarsal claw 0.37 mm (0.36–0.38).

Color Pattern (in alcohol). Chelicerae, pedipalps and carapace yellowish. Legs lighter colored. Abdomen pale yellow. Live animals with color pattern similar to the preserved specimens.

Genitalia ( Fig 4D ). Female gonopods cushion-like, with lateral projections directed backwards, covering almost entirely the atrium opening; projections (claws) not sclerotized, wide, and with a blunt apex, as in C. vulgaris (see Miranda and Giupponi [ 7]).

Natural history. Collected in the leaf litter.

Figures

Fig 2. A-H. Mesal view of the right chelicerae of all new species here described. A. Charinus brescoviti sp. n. (female paratype, IBSP 149); B. Charinus ricardoi sp. n. (female paratype, MZSP 22036); C. Charinus bonaldoi sp. n. (female paratype,MPEG 0061); D. Charinus guto sp. n. (female paratype, MZSP 41460); E. Charinus carajas sp. n. (male paratype, MZSP 28291); F. Charinus orientalis sp. n. (female paratype, MZSP 29118); G. Charinus ferreus sp. n. (female paratype, MZSP 29106); H. Charinus bichuetteae sp. n. (male paratype, MNRJ 9173). Scale bars: 1mm. doi:10.1371/journal.pone.0148277.g002

Fig 3. A-H. Trichobothria of left basitibia IV and distitibia IV. A. Charinus brescoviti sp. n. (female holotype, IBSP 149); B. Charinus ricardoi sp. n. (female holotype, MZSP 22036); C. Charinus bonaldoi sp. n. (female paratype, MPEG 0061); D. Charinus guto sp. n. (female holotype, MZSP 48146); E. Charinus carajas sp. n. (female holotype, MZSP 29136); F. Charinus orientalis sp. n. (female paratype, MZSP 29118); G. Charinus ferreus sp. n. (female holotype, MZSP 29104); H. Charinus bichuetteae sp. n. (male paratype, MNRJ 09173). Acronyms: bt, basotibial; bf, basofrontal; bc, basocaudal; sbf, sub-basofrontal; sc, caudal series; sf, frontal series;. Scale bars: 1mm. doi:10.1371/journal.pone.0148277.g003

Fig 4. A-H. Female and male gonopods of the new species described in this paper. A-F. Female gonopods. A. Charinus brescoviti sp. n. (IBSP 149); B. Charinus ricardoi sp. n. (MZSP 22036); C. Charinus bonaldoi sp. n. (MPEG 061); D. Charinus guto sp. n. (MZSP 48146); E. Charinus carajas sp. n. (MZSP 29132); F. Charinus orientalis sp. n. (MNRJ 09249). G-H. Male gonopods.G. Charinus carajas sp. n. (MZSP 29121); H. Charinus ferreus sp. n. (MZSP 29106). Scale bars of figures. A, B, C, E, G, H: 100μm. Scale bars of figures. D, F: 50μm. doi:10.1371/journal. pone.0148277.g004

Fig 7. A-E. Habitus and details of the sternum and pedipalp of Charinus guto sp. n. (female holotype, MZSP 48146) A. Dorsal habitus; B. Sternum; C. Frontal view of the left pedipalp basitiba, distitiba and claw; D. Dorsal view of the right pedipalp; E. Ventral view of the right pedipalp. Scale bars: 1mm.