Tegenaria barrientosi, Bolzern, Angelo, Crespo, Luís & Cardoso, Pedro, 2009

Bolzern, Angelo, Crespo, Luís & Cardoso, Pedro, 2009, Two new Te g e n a r i a species (Araneae: Agelenidae) from Portugal, Zootaxa 2068, pp. 47-58 : 48-52

Tegenaria barrientosi sp. n.

( Figs. 1–5 , 11–14 )

Type material. Holotype 3 (deposited at NMB, Nr.: 2804a, Figs 11–14 , collected by “modified Schoenly trap”); Portugal, Coimbra, Coimbra, “Jardim Botânico da Universidade de Coimbra” (40°12’, 8°25’W); leg. C. Prado e Castro, 2004. 4 3, 1 Ƥ, paratypes (deposited at NMB, Nr.: 2804b and 2804c, Figs 3–5 , collected by “modified Schoenly trap”); same locality and collecting details as holotype. 2 3, paratypes (deposited at the NMB, Nr.: 2804d, Figs 1–2 ); Portugal, Santarém, Ourém, Bairro (39°34’N, 8°37’W); leg. P. Cardoso, 18. June 2002. 1 3, paratype ( ZMUC, Nr.: ZMUC 00012596, collected by pitfall trap); Portugal, Santarém, Ourém, Bairro (39°34.'N, 8°37'W); leg. P. Cardoso, 23. April 2002.

Further material examined. 2 Ƥ (deposition at NMB, Nr.: 2804e, collected by pitfall traps. Specimens are in very bad condition; only epigynum of one female determinable), same locality; leg. L. Crespo, 6. June 2005.

Diagnosis. T. barrientosi sp. n. is closely related to T. incognita sp. n., T. bucculenta (L. Koch, 1868) , T. feminea (Simon, 1870) and T. montigena ( Simon, 1937) . It can be separated from the later three species by the absence of a patellar apophysis (present in T. feminea, Barrientos 1980 : fig. 1A), the broad and flat lateral tibial apophysis (fig. 2; absent in T. feminea ; much smaller in T. bucculenta, Barrientos 1991 : fig. 3; protruding ventrodistally in T. montigena, Simon 1937 : fig. 1541) and the presence of small diverticulae at the copulatory ducts (fig. 4). T. barrientosi sp. n. can be separated from T. incognita sp. n. by the pyramidal shaped ventral branch of the conductor (fig. 1; absent in T. incognita sp. n., fig. 6), the terminal end of the conductor pointing orthogonally away from the cymbium (fig. 2; pointing towards dorsal in T. incognita sp. n., fig. 7), the small diverticulae originating laterally and the irregularly sclerotized egg-shaped spermathecae which are touching each other anteriorly (fig. 3–4; diverticulae originating dorsally and smoothly sclerotized globular spermathecae which are clearly separated in T. incognita sp. n., figs 9–10).

range (in mm) of all examined specimens (n=7). The measurements of the holotype are given in parenthesis.

femur patella tibia metatarsus tarsus total

Pedipalp 0.95–1.38 0.46–0.61 0.47–0.64 - 1.00–1.42 2.88–3.94 (1.27) (0.61) (0.64) (1.42) (3.94)

femur patella tibia metatarsus tarsus total

Pedipalp 1.19–1.73 0.48–0.67 0.77–1.07 - 1.19–1.65 3.63–5.12 Leg I 2.49–3.65 1.02–1.42 2.15–3.15 2.02–3.04 1.34–1.77 9.02–13.03 Leg II 2.22 0.98 1.73 1.90 1.20 8.03

Leg III 2.07–3.00 0.88–1.23 1.54–2.27 2.12–3.03 1.17–1.67 7.78–11.20 Leg IV 2.76–4.00 1.00–1.42 2.42–3.42 3.06–4.38 1.24–1.98 10.48–15.20 Etymology. The species is named in honour of Dr. José Antonio Barrientos, who has been working on Agelenids of the Iberian Peninsula for the last 30 years; genitive case.

Description. Prosoma: carapace: 2.32–3.32 (3.06) mm long, 1.64–2.41 (2.41) mm wide in males (n=7); 2.82–3.85 mm long, 2.0– 2.68 mm wide in females (n=2). Fovea length to carapace length: 0.146–0.20 (0.177). Yellowish brown coloured without longitudinal darkened bands, border not darkened ( Fig. 11 ). Plumose hairs present. Head region: 0.96–1.39 (1.39) mm wide in males, 1.27–1.83 mm wide in females. Somewhat darker and separated from the thoracic region by a distinct sclerotized line. PER 0.49–0.66 (0.64) mm wide in males, 0.64–0.88 mm wide in females. Diameter of PME: 0.06–0.10 (0.09) mm; PLE: 0.08–0.13 (0.13) mm; AME: 0.0 6–0.0 8 (0.0 8) mm; ALE: 0.0 9–0.13 (0.13) mm. Eye formula ( Fig. 13 ): ALE=PLE>PME>AME. PME separated by 1.5 times their diameter. PME and AME separated by the diameter of PME or slightly more. PME and PLE separated by the diameter of PME or slightly more. PME and ALE separated by the diameter of PME or slightly more. AME separated by their diameter or slightly more. AME and ALE separated by 0.5–1 diameter of AME. Clypeus height (measured under AME): about 2.5–3.5 times the diameter of AME; clypeus height (measured under ALE): about 1–1.5 times the diameter of ALE. Both eye rows straight, or AER slightly recurved in dorsal view ( Fig. 11 ). AER slightly procurved or straight, PER strongly procurved in frontal view ( Fig. 13 ). Chelicerae: 1.05–1.66 (1.66) mm long, 0.44–0.75 (0.71) mm wide in males; 1.38–2.12 mm long, 0.75–1.08 mm wide in females. 3 teeth at the pro-margin (on one chelicerae of male paratype 4 teeth present), median tooth biggest. 7–12 teeth at the retro-margin, the proximal ones smaller. Gnathocoxa width to length: 0.456–0.524 (0.471). Labium width to length: 0.86–1.10 (0.945). Distal margin of labium concave. Sternum ( Fig. 12 ): 1.18–1.65 (1.63) mm long, 1.07–1.50 (1.48) mm wide in males; 1.40–2.15 mm long, 1.25–1.67 mm wide in females. Sternum with no colour pattern.

number of spines in the following order: Dorsal - prolateral - retrolateral - ventral. A “p” indicates that at this position a

pair of spines is present (1p = 2 spines at almost the same length). More than one spine formula per taxon and leg

segment indicates variation. A “*” indicates the occurrence only in males, “**” only in females.

species femur patella tibia metatarsus tarsus

Palp T. barrientosi sp. n. 2-0-0-0 2-0-0-0 2-2p-0-0 - many 1+1-0-2p-0*

T. incognita sp. n. 2-0-0-0 2-0-0-0 2-2p-0-0 - many

Leg I T. barrientosi sp. n. 2-2-0-0 2-0-0-0 0-0-0-3p** 0-0-0-4p+1 0 2-3-0-0 0-0-0-4p

2-0-0-4p

T. incognita sp. n. 2-1-0-0 2-0-0-0 0-0-0-1p+1* 0-0-0-1p+1+2p+1 0 2-2-0-0 0-0-0-1+1p* 0-0-0-4p+1* 0-0-0-1p+1+1p

0-0-0-1p+1+1p+1

0-0-0-2p+1

2-0-0-1p+1+1p

2-0-0-2p+1

Legs and palps: plumose hairs present. Yellowish-brown without pattern. Trochanter straight or slightly curved, not notched ( Fig. 12 ). Number of dorsal trichobothria at tarsus I and IV: 7–8; at tarsus II and III: 6–7.

Leg measurements are listed in tables 1–2. For spine formulae see table 3.

Opisthosoma: 2.18–3.04 (3.04) mm long, 1.41–1.89 (1.89) mm width in males; 3.85–4.04 mm long, 2.27–2.5 mm width in females. Colour is light yellowish brown-gray, no distinct pattern expressed ( Fig. 11 ). Plumose hairs present. Spinnerets ( Fig. 14 ): colulus rectangular shaped plate, pale. PLS longer than all others. PMS as long as ALS. ALS not darkened. Both segments of PLS pale. Distal segment of PLS as long as basal. Distance between PLS 1–2 times their diameter. Distance between ALS smaller than 0.5 their diameter.

Male palp ( Figs 1–2 ): femoral and patellar apophyses absent. RTA with 2 branches: lateral branch simple, robust and broad; lateroventral branch a long drawn-out lobe. Dorsally at palpal-tibia a short but robust spine present. Bulb length to cymbium length: 0.47–0.57. Embolus filiform, increasingly slender distally, less than ¾ of the cymbium width, curved through 90°. Embolus origin (free apex) on the left palp at 10½ o'clock position, terminal end at 3 o'clock position. Tegulum anterio-laterally not constantly curved, angular curve expressed close to embolus origin. Median apophysis pocket-like, consisting of a membranous basal part and terminally of a thin and broad sclerotized plate. Origin of median apophysis proximal at tegulum (between 5 and 7 o’clock position). Connection of median apophysis to tegulum basally sclerotized and fixed, more distally membranous. Conductor rectangular shaped, almost parallel to cymbium margin and shorter than the alveolus. Conductor folded only at the terminal end which is strongly sclerotized and pointing away from cymbium orthogonally. Additional pyramidal apophysis present (ventral branch of conductor) at the connection of tegulum and conductor. Connection of conductor to tegulum is membranous.

Epigynum and vulva ( Figs 3–5 ): whole epigynal plate 0.40–0.44 mm long, 0.71–0.72 mm wide, sclerotized. Atrium 0.16–0.22 mm long, 0.25–0.30 mm wide. Atrium only an indistinct, strongly sclerotized trapezoidal depression. Anterior margin of atrium visible as a continuous, little pronounced ridge. Posterior margin of the atrium reaches the epigastric furrow. Pair of posterior teeth present. Ducts and spermathecae barely visible through the plate. Copulatory openings visible as gaps, located at the anterior lateral margin of the atrium. Openings posterior and inwards directed. Copulatory duct short. Small laterally originating diverticula at the copulatory duct. Spermathecae strongly and irregularly sclerotized (some parts are stronger and bolder sclerotized than others) egg-shaped, touching each other anteriorly ( Fig. 5 ). Fertilisation ducts small.

Natural history. The species seems to prefer closed canopy forests, with a damp microclimate. Several adult specimens were captured between April and September, when the species seems to be most active.

Distribution. Only known from the two mentioned sites in the provinces of Santarém and Coimbra, Portugal ( Fig. 19 ).

Figures

FIGURE 19. Map of the collection localities of Tegenaria barrientosi sp. n. (black dots) and Tegenaria incognita sp. n. (black square) in Portugal.

FIGURES 1 – 5. Drawings of the reproductive organs of Tegenaria barrientosi sp. n.. 1 — left male palp in ventral view; 2 — left male palp in retrolateral view; 3 — epigynum in ventral view; 4 — vulva in ventral view; 5 — vulva in dorsal view. C = conductor; CD = copulatory duct; CO = copulatory opening; DV = DV = small diverticulum on the CD; E = embolus; ET = epigynal teeth; FD = fertilisation duct; MA = median apophysis; RTA = retrolateral tibia apophysis; S = spermathecae; T = tegulum; VC = ventral branch of conductor.

FIGURES 11 – 18. Photographs of a male Tegenaria barrientosi (11 – 14) and a female Tegenaria incognita (15 – 18). 11, 15 — habitus in dorsal view; 12, 16 — prosoma in ventral view; 13, 17 — eyes in frontal view; 14, 18 — spinnerets in ventral view. Scale bars are 1.0 mm in 11 – 12, 15 – 16, and 0.5 mm in 13 – 14, 17 – 18.