Diaporthe zaofenghuang X.H. Wang & G.P. Wang, 2021

Wang, Xianhong, Guo, Yashuang, Du, Yamin, Yang, Ziling, Huang, Xinzhong, Hong, Ni, Xu, Wenxing & Wang, Guoping, 2021, Characterization of Diaporthe species associated with peach constriction canker, with two novel species from China, MycoKeys 80, pp. 77-90 : 77

Diaporthe zaofenghuang X.H. Wang & G.P. Wang sp. nov. Fig. 4

Etymology.

Named after the host species ( Prunus persica cv. Zaofenghuang) from which the species was isolated.

Description.

Sexual morph not observed. Asexual morph on alfalfa stems. Pycnidial conidiomata conical, yellowish translucent conidial drops exuded from ostioles, 650-1430 μm diam. Conidiophores fasciculate, hyaline, long cylindrical, straight or slightly curved, apex pointed, 13.7-20.9 × 1.8-2.7 μm. Conidiogenous cells phialidic, cylindrical. Alpha conidia hyaline, aseptate, ellipsoidal, biguttulate, rounded at one end, slightly apex at another end, 5.3-7.5 × 2.9-3.7 µm (mean = 6.0 ± 0.6 × 3.1 ± 0.3 μm, n = 50). Beta and gamma conidia not observed.

Culture characteristics.

Cultures incubated on PDA at 25 °C in cycle of light/darkness, growth rate 8.5 mm per day. Colonies initially white on surface, producing black pigment from center of medium and expanding outwardly after 5-7 d. On PDA, edge of colony petal-like, irregular; on OA, edge relatively flat.

Materials examined.

China, Fujian Province, Sanming City, on buds of Prunus persica cv. Zaofenghuang , 23 March 2017, Y. S. Guo (holotype HMAS 249835, culture ex-holotype CGMCC3.20271 = TZFH1) ; ibid., culture TZFH3 .

Notes.

Two isolates representing D. zaofenghuang form a well-supported clade (1/100/100) and appear to be most closely related to D. penetriteum . Diaporthe zaofenghuang can be distinguished from D. penetriteum based on ITS, HIS, and TUB loci (10/578 in ITS, 45/520 in HIS, and 7/525 in TUB). Morphologically, D. zaofenghuang differs from D. penetriteum in having larger conidiomata ( D. zaofenghuang = 653-1433 μm vs D. penetriteum = 180-490 μm) and alpha conidia ( D. zaofenghuang = 6.0 ± 0.6 × 3.1 ± 0.3 μm vs D. penetriteum = 5.0 ± 0.3 × 2.2 ± 0.2 μm). Additionally, D. penetriteum produces two types of conidia, but D. zaofenghuang produces only alpha conidia ( Gao et al. 2016).

Figures

Figure 4. Diaporthe zaofenghuang. Front and back views of colonies on PDA (A, B) and OA (C, D), respectively E conidiomata on alfalfa stems F conidioma G conidiophores H alpha conidia I-J section view of conidioma. Scale bars: 2 mm (E); 200 μm (F); 20 μm (G, I); 10 μm (H, J).