Botryosphaeria fabicerciana (S.F. Chen, Pavlic, M.J. Wingf. & X.D. Zhou) A.J.L. Phillips & A. Alves

Rathnayaka, Achala R., Chethana, K. W. Thilini, Phillips, Alan J. L. & Jones, E. B. Gareth, 2022, Two new species of Botryosphaeriaceae (Botryosphaeriales) and new host / geographical records, Phytotaxa 564 (1), pp. 8-38 : 31-33

Botryosphaeria fabicerciana (S.F. Chen, Pavlic, M.J. Wingf. & X.D. Zhou) A.J.L. Phillips & A. Alves , Stud. Mycol. 76: 77 (2013)

Index Fungorum number: IF 805457; Facesoffungi number: FoF 09152, Fig. 14

Saprobic on dead branches of Prunus serrulata . Sexual morph: Ascomata 200–270 μm high, 200–255 μm diam. (x̄ = 230 × 235 μm, n = 10), dark brown to black, solitary to aggregated, semi-immersed, erumpent at maturity, uniloculate, globose to subglobose, ostiolate. Peridium 20–56 μm wide, two-layered, outer layer composed of brown to dark brown, thick-walled cells of textura angularis, inner layer composed of hyaline, thin-walled cells of textura angularis. Hamathecium composed of 2–6 μm wide, dense, hyphae-like, branched, septate, hyaline pseudoparaphyses. Asci 105–132 × 15–26 μm (x̄ = 120 × 23 μm, n = 10), bitunicate, fissitunicate, 8-spored, clavate to cylindro-clavate, short pedicellate, apically rounded with a well-developed ocular chamber. Ascospores 18–24 × 8–12 μm (x̄ = 20 × 9 μm, n = 20), 1−2-seriate, hyaline, aseptate, ellipsoidal to fusiform, usually wider in the centre, thick and rough-walled, guttulate. Asexual morph: see Rathnayaka et al. (2021) for description.

Culture characteristics:—Ascospores germinating on PDA within 6 hours and germ tubes produced from one side or both sides of the ascospore. Colonies on PDA reaching 5–7 cm diam. after 4 days at 25 °C, circular, flattened, fluffy, fairly dense, aerial, white at first, becoming grey to grey-black in the front view and black in reverse.

Material examined: — TAIWAN. Alishan Mountain, Chiayi: on a dead twig of Prunus serrulata (Rosaceae) , 16 August 2019, Achala Rathnayaka (MFLU 22-0097, new host record), living culture NCYUCC 19-0405.

Known hosts and distribution:— Avicennia marina in South Africa ( Osorio et al. 2017), Camellia sinensis in Taiwan ( Rathnayaka et al. 2021), Carya cathayensis in China ( Wang et al. 2014), Caryota sp. and Entada sp. in Thailand ( Liu et al. 2012), Eucalyptus sp. in China ( Chen et al. 2011), Mangifera indica in Brazil ( Marques et al. 2013, Nogueira et al. 2016), South Africa ( Mehl et al. 2017), Taiwan ( Burgess et al. 2019) and USA ( Zhang et al. 2021), Morus nigra in Brazil ( Silva et al. 2021), Syzygium cordatum in South Africa ( Zhang et al. 2021), Prunus serrulata in Taiwan (this study).

Notes:—Our strain (NCYUCC19-0405) clustered with the ex-type and other strains of Botryosphaeria fabicerciana (CMW27094, CMW 27108, MFLU 20-0526 and MFLUCC 10-0098) with high support (82% ML/ 0.99 PP) based on the multi-gene phylogenic analyses (ITS, tef 1-α and β-tub) ( Fig. 4 ). Morphologically, our strain is similar to the B. fabicerciana holotype (CMW27094) collected from twigs of an unknown Eucalyptus sp. in China ( Chen et al. 2011), in having dark brown to black, erumpent ascomata, and hyaline, aseptate, ellipsoidal to fusiform ascospores ( Liu et al. 2012). However, the holotype has aseptate pseudoparaphyses, while our strain has septate pseudoparaphyses ( Liu et al. 2012). Based on morpho-molecular data analysis, we conclude that our new collection is a new host record of B. fabicerciana on Prunus serrulata in Taiwan.

Figures

FIGURE 4. Phylogram generated from ML analysis basedon combined datasetof ITS, tef1-α and β-tub. The tree is rooted toMacrophomina phaseolina (CBS 227.33 and CBS 162.25). Tree topology is similar to previous study conducted by Rathnayaka et al. (2021). Bootstrap support values for ML ≥ 75% and Bayesian posterior probabilities (PP) ≥ 0.90 are noted at the nodes. Strain numbers are noted after the species names. The strain isolated in this study is presented in red and type strains are in bold.

FIGURE 14. Botryosphaeria fabicerciana on dead twig of Prunus serrulate (NCYUCC 19-0405). a, b. Appearance of ascostromata on host surface. c. Section through ascoma. d. Peridium. e. Pseudoparaphyses. f–h. Asci. i–l. Ascospores. m. Germinating ascospore. n, o. Colony on PDA (n upper, o lower). Scale bars: a = 500 μm, b, f–h = 50 μm, c = 100 μm, d, e, m = 20 μm, i–l = 10 μm.