Wongia fusiformis D.F. Bao, H.Y. Su, K.D. Hyde & Z.L. Luo.

Zhang, Liang, Shen, Hong-Wei, Bao, Dan-Feng, Li, Long-Li, Su, Xi-Jun & Luo, Zong-Long, 2023, Wongia suae sp. nov., a lignicolous freshwater fungus from Yuanjiang (Red River) Basin, China, Phytotaxa 616 (3), pp. 258-268 : 262-263

Wongia fusiformis D.F. Bao, H.Y. Su, K.D. Hyde & Z.L. Luo. ,

Journal of Fungi 7: 669. (2021), FIGURE 2

Index Fungorum number: IF558595; Facesoffungi number: FoF 09917.

Saprobic on submerged decayed wood in freshwater habitats. Sexual morph: Undetermined. Asexual morph: Colonies on superficial substratum, hairy, scattered, granulate and black. Mycelium partly superficial and partly immersed, composed of branched, septate, smooth and with brown to pale brown hyphae. Conidiophores 97–154 × 3–4 µm, (x = 125 × 4 µm, n = 10), macronematous, mononematous, simple, solitary, septate, erect, straight or slightly flexuous, dark brown, paler towards apex and smooth-walled. Conidiogenous cells polyblastic, denticulate, terminal, sympodial, subhyaline to pale brown. Conidia 14–18 × 4–5 µm (x= 16 × 5 µm, n = 30), acropleurogenous, solitary, fusiform, clavate, tapering and pointed at both ends, (1)–3-septate, pale brown to brown, slightly darker at middle cell and paler at end cells, smooth-walled.

Culture characteristics: Conidia germinating on PDA within 24 h and germ tubes produced from the basal and apical cell of conidia. Colonies grew on PDA, reaching 31–33 mm in three weeks at 25°C, rough surface, with dense mycelia, velvety, dry, umbonate in the middle from the side view, edge undulate, brown to dark brown from front, dark brown from reverse and not producing pigmentation in culture.

Material examined:— CHINA, Yunnan Province, Yuxi City, on submerged decaying wood in freshwater stream in Yuanjiang basin, February 2022, Hong-Wei Shen, S-3682 (KUN-HKAS 102464), living cultures, CGMCC 3.24254 = KUNCC 22-2676 ; ibid. on submerged decaying wood in freshwater stream in Yuanjiang basin, February 2022, Hong-Wei Shen, S-3374 (KUN-HKAS 102463), living culture, KUNCC 22-12675 .

Notes: Wongia fusiformis was introduced by Bao et al. (2021) based on morphological and phylogenetic analyses. In our phylogenetic analysis, the two new collections (KUNCC 22-12675 and KUNCC 22-12676) clustered with W. fusiformis with 100 ML/1.00 PP support ( FIGURE 1 ). The morphology of our new isolates fits W. fusiformis well. We, therefore, identified them as W. fusiformis . However, conidia of our new isolates have 1–3-septate, while the holotype has conidia with 1–2-septate. This may due to the insufficient photography, which could not provide enough morphological information for the fungus ( Bao et al. 2021).

Figures

FIGURE 1. RAxML tree based on analysis of combined ITS, LSU, rpb2, SSU and tef1-α dataset. RAxML bootstrap support values equal to or greater than 75% are given before the forward slash. Branches with bayesian posterior probabilities equal to or higher than 0.95 are given after the forward slash. The tree is rooted to Barbatosphaeria barbirostris (AFTOL-ID 967). The ex-type strains are in bold. The newly obtained sequences are indicated in red.

FIGURE 2. Wongia fusiformis (KUN-HKAS 102464). (a) Colonies on submerged decaying wood; (b–e) Conidiophores and conidia; (f–h) Conidiogenous cells; (i–n) Conidia; (o) Germinating conidium; (p, q) Culture on PDA from front and reverse. Scale bars: (b–d) 40 µm; (e) 30µm; (f–o) 10 µm.