Thalassaphorura problematica, Sun, Xin, Deharveng, Louis & Wu, Donghui, 2013

Sun, Xin, Deharveng, Louis & Wu, Donghui, 2013, Broadening the definition of the genus Thalassaphorura Bagnall, 1949 (Collembola, Onychiuridae) with a new aberrant species from China, ZooKeys 364, pp. 1-9 : 2-4

Thalassaphorura problematica sp. n.

Type material.

Holotype female; paratypes 9 females and 3 males on slides-China, Heilongjiang: Wulindong town, 46°33'N, 133°40'E, 16 Aug 2010, forest soil and humus, Wu Donghui, Liu Dong, Yuan Xiaoqiang and Yuan Yabin leg.

Diagnosis.

Pso formula as 32/133/33343 dorsally, 11/000/00010 ventrally; psx formula as 0/000/112001+1m ventrally; Ant. III sensory organ with two granulated clubs (inner one bigger than outer); labium with 5 proximal chaetae; labial type AB; tibiotarsi of legs I–III with 14 (1, 7, 6) chaetae each; male ventral organ present on ventral tube as modified distal chaetae; anal spines 1.1-1.2 times as long as inner edge of hind unguis.

Description.

Body white in alcohol. Size 1000-1300 µm in females, 800-1100 µm in male; holotype: 1050 µm. Body subcylindrical, body sides parallel.

Pseudocellar formula: 32/133/33343 dorsally, 11/000/00010 ventrally, subcoxa 1 of legs I-III with 2, 2 and 2 pso respectively (Fig. 1A, B). Parapseudocellar formula: 0/000/112001+1m (each of anal valve with one psx) ventrally, absent dorsally (Figs 1A, B, 2G). Pseudopore formula: 0/011/11110 dorsally, 00/111/0001m0 ventrally (Fig. 1A, B).

Head. Antennae short and distinctly segmented, as long as head. Length ratio of Ant. I: II: III: IV as about 1: 1.5: 1.5: 1.5. Subapical organite of Ant.IV with globular apex; basolateral ms at about 1/3 length from base, above the second proximal row of chaetae (Fig. 1F). Ant. III sensory organ composed of 5 papillae, 5 guard chaetae, 2 sensory rods and 2 granulated clubs, the inner bigger than the outer, and a lateral ms (Figs 1D, F). Ant. II with 13 chaetae. Ant. I with 8 chaetae. Antennal base well marked. PAO composed of 20-24 simple vesicles (Fig. 1C). Dorsal cephalic chaeta d0 absent (Figs 1A, 2A). 3+3 p-chaetae present between two inner posterior pso, p1 anterior to others. Mandible with strong molar plate and 4 apical teeth. Maxilla bearing 3 teeth and 6 lamellae. Maxillary palp simple with 1 basal chaeta and 2 sublobal hairs. Labral formula 4/1,4,2;. Labium with 5 proximal, 4 basomedian (E, F, G, f) and 5 basolateral (b, c, d, e, e’) chaetae (Fig. 2B); labial type AB, papillae A–E respectively with 1, 4, 0, 3 and 2 guard chaetae (Fig. 1E). Head ventrally with 4+4 postlabial chaetae along ventral groove (Fig. 2B).

Body chaetotaxy. S-chaetae subcylindrical, apically rounded, 11/011/222121 dorsally, 11/000/000110 ventrally (Figs 1A, B); subcoxae 2 of legs I, II and III with 0, 0, 1 S-chaeta respectively. Tiny and blunt ms, present on Th. II–III. Ordinary chaetae differentiated into meso- and macrochaetae, ratio Sp: m1: p1 on Abd. V tergum = 1: 2-2.3: 0.8. Th. I tergum with 7 –8+7– 8 dorsal chaetae. Th. II–III terga with 4+4 chae tae and Abd. I–III terga with 3+3 chaetae along axis respectively (Fig. 1A). Abd. IV–V terga with one axial chaeta (p0) each, sometimes with asymmetric chaetae along axis. Abd. VI tergum with two axial chaetae (a0 and p0) (Figs 1A, 2C). Sterna of Th. I, II, and III with 0+0, 1+1, 1+1 chaetae respectively.

Appendages. Subcoxa 1 of legs I–III with 4, 5 and 5 chaetae, subcoxa 2 with 0, 4 and 4 chaetae respectively. Tibiotarsi of legs I, II and III with 14 (1, 7, 6) chaetae each (Fig. 2E). Unguis without teeth. Unguiculus short, about 0.3 times as long as inner edge of unguis, with inner basal lamella (Fig. 2E). Ventral tube with 1+1 basal and 8 –11+8– 11 distal chaetae (8 –10+8– 10 in female, 11+11 of which 9+9 modified in males) (Fig. 2D). Furca reduced to a field of fine granulation with 4 small dental chaetae arranged in 2 rows posteriorly; only one manubrial row of chaetae present posteriorly to dental chaetae (Fig. 2F).

Genital plate with 14-15 chaetae in females, 33-36 chaetae in male. Anal valves with numerous acuminate chaetae; each lateral valve with a0 and 2a1; upper valves with chaetae a0, 2b1, 2b2, c0, 2c1, 2c2 (Fig. 2G). Anal spines set on distinct papillae, 1.1-1.2 times as long as inner edge of hind unguis.

Derivatio nominis.

Named for its unusual characters among Thalassaphorura .

Discussion.

The new species is closest to the genus Thalassaphorura by its simple vesicles in PAO and the furcal rudiment. However, it does not match the definition of this genus proposed by Sun et al. (2010), nor those given previously by Weiner (1996), Fjellberg (1999) or Pomorski (1998) for three characters: absence of chaeta d0 on head, 6 chaetae in the distal whorl of tibiotarsi of all legs, and labium type AB. In order not to erect a new genus in a tribe in need of revision ( Sun et al. 2011) and for a species otherwise very similar to existing Thalassaphorura , we placed our new species in the genus Thalassaphorura and broadened its diagnosis.

The new species belongs to the species-group of Thalassaphorura which has modified ventral chaetae in the adult male ("male ventral organ"), including the species Thalassaphorura petiti Sun & Wu, 2013, Thalassaphorura bisetosa Sun & Wu, 2013, Thalassaphorura qinlingensis Sun & Wu, 2013, Thalassaphorura macrospinata Sun & Wu, 2012 and Thalassaphorura qixiaensis Yan, Shi & Chen, 2006, all described from China. These species can be distinguished easily by the position or the number of modified chaetae of the male ventral organ, dorsal and ventral pso formula, and ventral psx formula.

Assigning the new species to this genus led us to re-examine three important taxonomic characters that separate the new species from most other Thalassaphorura .

The distal tibiotarsal chaetae have been recently checked in the genera Allonychiurus , Onychiurus and Thalassaphorura ( Sun et al. 2010; Sun et al. 2011; Sun and Zhang 2012), showing that this character has a limited taxonomical value to discriminate these genera. In addition, paratypes of Thalassaphorura petaloides (Rusek, 1981) from Iraq and specimens of the same species from southern China were found to actually have 15 (1, 7, 7), 14 (1, 7, 6) and 14 (1, 7, 6)) chaetae on tibiotarsi I, II and III. Together with reduced tibiotarsal chaetotaxy of the new species described here, this leads us to extend the diagnosis of Thalassaphorura to species with 6, 7 or 9 chaetae in the distal row of tibiotarsus.

Chaeta d0 on head is considered as a stable character at the generic level. It is present in all species of Thalassaphorura ( Sun et al. 2011) except Thalassaphorura jailolonis (Yoshii & Suhardjono, 1992) from Malukku (Indonesia) and the new species Thalassaphorura problematica sp. n. The species Thalassaphorura jailolonis was described in Jailolaphorura Yoshii & Suhardjono, 1992 (a subgenus of Onychiurus , upgraded to genus level by Weiner in 1996), but was subsequently transferred to Thalassaphorura by Bellinger et al. (1996-2013) according to a personal communication of Pomorski in 2002. This assignation is however uncertain because the chaetotaxy of the furcal rest is unknown in Thalassaphorura jailolonis . At this point, we consider that the diagnosis of the genus Thalassaphorura should provisionally state that d0 is present or absent on head, waiting for a re-examination of Thalassaphorura jailolonis on fresh material.

The third character, labium type, is not stable in several genera of Thalassaphorurini , being AC or ABC in Allonychiurus and Sensillonychiurus ( Babenko et al. 2011), and even A, AC or ABC in Thalassaphorura ( Sun et al. 2010). In our new species, labium is still of another type - AB. Moreover, labial type is undescribed in many species. This high intra-generic variability implies that this character should not be considered diagnostic at a generic level among Thalassaphorurini .

An amended diagnosis of the genus Thalassaphorura and an updated key of the genera of Thalassaphorurini integrating these changes are given below.