Phaeoisaria laianensis Y. Liu, G.P. Xu, X.Y. Yan, D.M. Hu & Z.J. Zhai, 2022

Liu, Yu, Xu, Gui-Ping, Yan, Xin-Yi, Chen, Min-Hui, Gao, Yang, Hu, Hai-Jing, Song, Hai-Yan, Hu, Dian-Ming & Zhai, Zhi-Jun, 2022, Phaeoisaria laianensis (Pleurotheciales, Pleurotheciaceae), a new species from freshwater habitats in China, Biodiversity Data Journal 10, pp. 94088-94088 : 94088

Phaeoisaria laianensis Y. Liu, G.P. Xu, X.Y. Yan, D.M. Hu & Z.J. Zhai sp. nov.

Materials

Type status: Holotype. Occurrence: recordedBy: Yu Liu; occurrenceID: 99B9C819-CA87-5634-AB08-7E7A79E1ADE0; Taxon: scientificName: Phaeoisaria laianensis; acceptedNameUsage: Phaeoisaria laianensis Y. Liu, D.M. Hu & Z.J. Zhai; kingdom: Fungi; phylum: Ascomycota; class: Sordariomycetes; order: Pleurotheciales; family: Pleurotheciaceae; genus: Phaeoisaria; specificEpithet: laianensis; taxonRank: species; verbatimTaxonRank: species; scientificNameAuthorship: Y. Liu, D.M. Hu & Z.J. Zhai; Location: continent: Asia; country: China; stateProvince: Anhui; county: Laian; locality: Wawuzhuang ; verbatimElevation: 35; locationRemarks: Label transliteration; verbatimCoordinates: 32.66 N, 118.65 E; verbatimLatitude: 32.66; verbatimLongitude: 118.65; Identification: identifiedBy: Yu Liu and Zhi-jun Zhai; dateIdentified: 2021; Event: samplingProtocol: collecting; eventDate: 06-05-2021; year: 2021; month: 5; day: 6; habitat: Freshwater; Record Level: type: PhysicalObject ; language: en; rightsHolder: Dian-Ming Hu and Zhi-jun Zhai ; institutionID: HFJAU10040; collectionID: LKJ17; institutionCode: the Herbarium of Fungi , Jiangxi Agricultural University (HFJAU); collectionCode: Fungi ; ownerInstitutionCode: HFJAU

Description

Saprobic on decaying wood submerged in freshwater habitats. Sexual morph: Undetermined. Asexual morph: Colonies effuse, solitary, scattered, dark brown to black, hairy, covered by white conidial mass. Mycelium partly superficial, partly immersed. Synnemata 290-848 × 9.3-30.7 µm (x̅ = 532 × 18.6, SD = 159 × 5, n = 20), erect, rigid, dark brown to black, velvety, smooth, composed of compactly and parallel adpressed conidiophores. Conidiophores 116.2-491.1 × 2-3.2 µm (x̅ = 276.1 × 2.4, SD = 96.7 × 0.5, n = 10), macronematous, synnematous, septate, branched, brown to dark brown, smooth. Conidiogenous cells 8.3-27.5 × 2.3-3.8 µm (x̅ = 17.1 × 2.7, n = 10), polyblastic, integrated, terminal, hyaline to pale brown, smooth, denticulate, sympodial, each with several denticulate conidiogenous loci, 0.8-1.6 × 0.4-0.8 µm (x̅ = 1.3 × 0.7, n = 10). Conidia 5-7.2 × 1.7-2.9 µm (x̅ = 5.9 × 1.7, SD = 0.5 × 0.3, n = 50), ellipsoidal to obovoid, straight, rounded at the apex, obtuse and tapering towards base, hyaline, aseptate, guttulate, smooth-walled. (Fig. 1 ).

Culture characteristics: Conidia germinated within 24 h in which germ tubes were produced from both ends or sides at 28℃ on PDA. The colony on PDA grows up slowly and reaches 24.5 mm in 26 days, periphery grey, surface folded, middle grey-green to black, raised with mycelium in the centre, covered with lots of white conidia, powdery, reverse grey to black.

Material examined: China, Anhui Province, alt. 35 m, near 32.66°N, 118.65°E, on decaying wood submerged in a freshwater stream, 6 May 2021, Y. Liu, G.P. Xu and Z.J. Zhai, LKJ17 (HFJAU 10040, holotype), ex-type living culture, CCTCC AF 2022069 = CCTCC AF 2022073.

Etymology

The name reflects the district where this fungus was found.

Notes

Phylogenetic analysis shows that Phaeoisaria laianensis is a phylogenetically-distinct species, most closely related to P. dalbergiae and then to P. clematidis (Fig. 2 ). However, P. laianensis is easily distinguished from P. dalbergiae by its ellipsoidal to obovoid, rounded at the apex and tapering towards base conidia ( Crous et al. 2021). In addition, P. laianensis has synnemata, which is absent in P. dalbergiae ( Crous et al. 2021), also in P. curvata , P. glauca ( de Hoog and Papendorf 1976), P. loranthacearum ( Crous et al. 2015), P. annesophieae and P. fasciculata ( Réblová et al. 2016) (Table 2 ). The new species is similar to P. clematidis in having resembling synnemata or conidia ( Hughes 1958, Luo et al. 2018), while the former has shorter synnemata (290-848 µm vs. 1000-1500 µm) and smaller conidia (5-7.2 µm wide vs. 4-10 µm wide) than P. clematidis (Table 2 ). Likewise, P. laianensis has longer synnemata than P. siameneis (290-848 µm vs. 330-380 µm), smaller conidiophores than P. guttulata ( Hyde et al. 2018) and P. aquatica (116.2-491.1 × 2-3 µm vs. 480-700 × 2-5 µm and 1028-1262 × 3.5-4.5 µm) ( Luo et al. 2018) and smaller conidia (5-7.2 × 1.7-2.9 µm) than P. annesophiae (4.5-9 × 2-3.5 µm) ( Crous et al. 2017), P. synnematica (4-11 µm long) ( Boonmee et al. 2021) and P. siamensis (3-4 µm wide) (Table 2 ). In addition, it can be differentiated from P. filiforms by the indeterminate asexual morph of the latter species ( Luo et al. 2019).

Figures

Figure 1. Phaeoisaria laianensis (HFJAU 10040, Holotype) a, b Colonies on wood; c, d Conidiophores; e, f Conidiogenous cells with conidia; g Germinating conidium; h Conidia; i, j Colony on PDA for 26 days from above and reverse. Scale bars: a, b = 100 µm, c, d = 50 µm, e-h = 10 µm.

Figure 2. Phylogenetic tree of Bayesian analysis, based on a concatenated alignment of ITS, LSU and SSU sequences. Branch support is shown at the nodes, Maximum Likelihood bootstrap support (BS, black) ≥ 60 % and Bayesian posterior probability (PP, red) ≥ 0.95. Leotialubrica (AFTOLID 1) and Microglossum rufum (OSC 100641) are selected as the outgroup taxa. The new species is marked in red and ex-type strains are in bold.