Paramphibambusa bambusicola L.S. Han & D.Q. Dai, 2024

Han, Li-Su, Wijayawardene, Nalin N., Liu, Chao, Han, Li-Hong, Promputtha, Itthayakorn, Li, Qiang, Elgorban, Abdallah M., Al-Rejaie, Salim, Tanaka, Kazuaki & Dai, Dong-Qin, 2024, Paramphibambusa bambusicola gen. et. sp. nov., Arecophila xishuangbannaensis and A. zhaotongensis spp. nov. in Cainiaceae from Yunnan, China, MycoKeys 104, pp. 113-132 : 113

Paramphibambusa bambusicola L.S. Han & D.Q. Dai sp. nov.

Fig. 2

Etymology.

With reference to its occurrence on host bamboo.

Holotype.

GMB-W1350.

Description.

Saprobic on dead culms of bamboo. Sexual morph: Ascomata 430-580 × 500-550 µm (x- = 474 × 519 µm, n = 20), deeply immersed beneath blackened poorly developed clypeus, solitary, scattered, black, globose to subglobose, ostiolate, with a long neck, 50-125 µm diam., 240-260 µm long. Peridium 15-25 µm thick, composed of several layers, thick-walled, hyaline to pale brown cells of textura angularis. Paraphyses 2-5.5 µm wide, hyaline, numerous, filiform to cylindrical, guttulate, branched, septate, tapering towards the apex. Asci 200-240 × 10-13.5 µm (x- = 215 × 11.5 µm, n = 20), 8-spored, rarely 6-spored, unitunicate, cylindrical, short pedicellate, straight or slightly curved, rounded at the apex, with a 3-4 µm wide, 1.5-2 µm high (x- = 3.6 × 1.7 µm, n = 20), elliptical to trapezoidal, J+, sub-apical ring. Ascospores 24-35 × 6-7.5 µm (x- = 27 × 6.6 µm, n = 20), uniseriate or overlapping uniseriate, hyaline to golden brown, ellipsoidal, 2-3-celled, tapering at the ends, slightly constricted at the septum, smooth-walled, surrounded by a 9-12 µm mucilaginous sheath. Asexual morph: Undetermined.

Culture characters.

Ascospores germinating within 24 h. Colonies reaching 45 mm diam. in 20 days under dark and at 28 °C conditions, circular, flocculent, yellowish from above and below.

Materials examined.

China, Yunnan Province, Zhaotong, Zhenxiong town, 27°36′8"N, 104°56′34"E, 1673.07 m, on dead culms of bamboo, 29 July 2021, Dong-Qin Dai, Li-Su Han, DDQ02077, (GMB-W1350, holotype), GMBCC1142, ex-type; ibid. (ZHKU 23-0256, isotype), GZCC 23-0629, ex-isotype; Zhaotong, Zhenxiong town , Shanzhai, 27°62′52"N, 104°81′98"E, 1666.10 m, on dead culms of bamboo, 4 August 2023, Dong-Qin Dai, Li-Su Han, HLS0114 (ZHKU 23-0257), living culture Z HKUCC 23-0976 .

Notes.

In the phylogenetic tree, Paramphibambusa bambusicola formed a stable clade basal to the other species of Cainiaceae with 90% ML, and 1.00 PP statistical supports (Fig. 1 ). In morphology, Paramphibambusa bambusicola has Cainiaceae species typical characteristics that are cylindrical asci, with a J+, apical ring, and ellipsoidal ascospores surrounded by a mucilaginous sheath. However, the spores of Cainiaceae species have the ornamented walls with longitudinal striations or germ slits or germ pores. Paramphibambusa bambusicola differs from the current Cainiaceae species by having smooth-walled ascospores. Therefore, based on morphological and phylogenetic studies, P. bambusicola is introduced hereby as a new species occurring on bamboo in Yunnan, China.

Figures

Figure 1. The RAxML tree was generated based on the combined ITS, LSU, rpb 2, and tub sequence data. Bootstrap support values for ML equal to or greater than 60 %, and Bayesian posterior probabilities (BYPP) equal to or higher than 0.90 are indicated above the nodes as ML / PP. Type materials are indicated by superscript " T ", while the newly generated sequences are shown in red.

Figure 2. Paramphibambusa bambusicola (GMB-W 1350, holotype) a bamboo specimen b black ostioles at the host surface c transverse section of ascomata d, e vertical section of ascomata with long necks and black clypeus f cells of peridium g paraphyses h-k asci l asci with J +, elliptical to trapezoidal, subapical ring (stained in Melzer's reagent) m-s ascospores (s ascospore stained in Indian ink showing mucilaginous sheath) t a germinating ascospore u, v cultures on PDA after 20 days (u upper, v reverse). Scale bars: 300 µm (d, e); 15 µm (f, l-t); 30 µm (g); 50 µm (h-k).