Neopestalotiopsis longiappendiculata

Kwon, Sun Lul, Cho, Minseo & Kim, Changmu Kim and Jae-Jin, 2024, First report of seven unrecorded bambusicolous fungi in Korea, Journal of Species Research 13 (2), pp. 111-126 : 122

Neopestalotiopsis longiappendiculata

E. Diogo, M.H. Bragança & A.J.L. Phillips ,

Mycol. Progr. 20(11): 1450(2021) ( Figs. 4 , 11 )

MycoBank. MB 839460

On PDA, colonies thick, circular form, margin entire, aerial mycelia abundant, mycelia white. Yellow (10YR 7/6) pigment diffused in media. Conidiomata pycnidia, black, rare. Conidiophores reduced to conidiogenous cells. Conidiogenous cells ampulliform to subcylindrical, hyaline, thin-walled, smooth, (8.0-)10-12.5 (-16) × (3.5-) 4.5-6.0(-7.0) μm. Conidia fusiform to ellipsoidal, straight or slightly curved, 4-septate, (16.0-)19-23(-25) × (6.0-) 6.5-7.5(-8.0) μm; basal cell obconic to subcylindrical, (2.5-)3.5-5.0 μm long, with a single filiform appendage, hyaline, thin-walled, 3.0-7.0(-9.0) μm long; apical cell conical, (3.0-)3.5-4.5(-5.0) μm long, with 2-4 filiform apical appendage, hyaline, flexuous, thin-walled, (19-) 20-27(-30) μm long; three median cells doliiform, (12.5-)13-14.5(-15.5) μm long, third septa from the base darker than rest cell (second cell from base pale brown, (3.5-)4.0-5.0 μm long; third cell from brown to dark brown, (3.0-)4.0-5.5(-6.5) μm long; fourth cell from base brown, 4.0-6.0(-6.5) μm long).

Distribution. Portugal and Republic of Korea.

Habitat. On leaves and stems of Eucalyptus globulus and E. nitens , and dead leaf of Phyllostachys bambusoides .

Specimen examined. KOREA, Jeju-do, Pyoseon-myeon, Maeoreum , 33.3179N, 126.8132E, isolated from a dead leaf of Phyllostachys bambusoides , 15 Apr 2021, Jae-Jin Kim, KUC21859(= NIBRFGC000510475) .

Notes. The morphological features of KUC21859 are well-matched with the original description ( Diogo et al., 2021). Therefore, the bambusicolous fungi KUC21859 was successfully identified as N. longiappendiculata by integrating both the morphology and phylogenetic results. This is the first report of N. longiappendiculata species living on the dead bamboo leaf. This species was reported as a plant pathogen causing grey blight disease in tea plants and Camellia species ( Wang et al., 2022b).

Figures

Fig. 4. Maximum Likelihood tree for genus Neopestalotiopsis with ITS+TEF1-α+TUB dataset. Node numbers indicate bootstrap value above 70%. Blue colored names indicate the strains isolated in this study. Type strains are indicated by “T”.

Fig. 11. Morphology of Neopestalotiopsis longiappendiculata (KUC21859). (A, B) colony on PDA(above and reverse); (C, D) conidia; (E) conidiogenous cells. Scale bars: 10μm (C–E).