Neopestalotiopsis iberica

Kwon, Sun Lul, Cho, Minseo & Kim, Changmu Kim and Jae-Jin, 2024, First report of seven unrecorded bambusicolous fungi in Korea, Journal of Species Research 13 (2), pp. 111-126 : 119-122

Neopestalotiopsis iberica E. Diogo, M.H. Bragança & A.J.L. Phillips ,

Mycol. Progr. 20(11): 1449(2021) ( Figs. 4 , 10 )

MycoBank. MB 839459

On PDA, colonies thick, circular form, margin curled to undulate, aerial mycelia fluffy, abundant, mycelia white. Yellow (10YR 8/6) pigment diffused in media. Conidiomata abundant. Conidiomata pycnidial, black, abundant. Conidiophores reduced to conidiogenous cells. Conidiogenous cells ampulliform to lageniform, hyaline, thin-walled, smooth, (7.0-)9.0-11.5(-14.5) × (3.0-)4.0-5.0 (-6.5) μm. Conidia ellipsoidal, straight, 4-septate, (18.5-) 20-24.5(-27.5) × (5.5-)6.0-6.5(-7.0) μm; basal cell obconic to subcylindrical, (4.0-)4.5-6.0(-7.0) μm long, with a single filiform appendage, hyaline, thin-walled, (2.5-)6.5-10(-15) μm long; apical cell subcylindrical to conical, (2.5-)3.5-5.5(-6.0) μm long, with 2-3 filiform apical appendage, hyaline, flexuous, thin-walled, (8.5-) 9.5-17(-19.5) μm long; three median cells doliiform, (11-)12.5-15(-16) μm long, third septa from the base darker than rest cell (second cell from base pale brown, 4.0-5.5(-5.8) μm long; third cell from dark brown, (3.0-) 4.0-5.0 μm long; fourth cell from base brown, (3.5-)4.0- 5.5(-6.0) μm long).

Distribution. Portugal, Spain, and Republic of Korea.

Habitat. On leaves and stems of Eucalyptus globulus , leaves of symptomatic Camellia oleifera , and culm of Phyllostachys bambusoides .

Specimen examined. KOREA, Jeju-do, Pyoseon-myeon, Maeoreum , 33.3179N, 126.8132E, isolated from a culm of Phyllostachys bambusoides , 15 Apr 2021, Jae-Jin Kim, KUC21856 (= NIBRFGC000510474) .

Notes. The morphological features of KUC21856 were slightly different by narrow conidia, long basal cells, long basal cell attached appendages, and short apical attached appendages from the original description ( Diogo et al., 2021). However, other features (e.g., culture characteristics and conidiogenous cells) and phylogeny indicated that the strain KUC21856 is N. iberica . This is the first report of N. iberica species living on the culm of P. bambusoides .

Figures

Fig. 4. Maximum Likelihood tree for genus Neopestalotiopsis with ITS+TEF1-α+TUB dataset. Node numbers indicate bootstrap value above 70%. Blue colored names indicate the strains isolated in this study. Type strains are indicated by “T”.

Fig. 10. Morphology of Neopestalotiopsis iberica (KUC21856). (A, B) colony on PDA (above and reverse); (C, D) conidia; (E) conidiogenous cells. Scale bars: 20μm (C, E); 10 μm (D).